6.5-66 kDa)
(Product Number M 3913)
SigmaMarker, High Range (36-205 kDa)
(Product Number M 3788)
SigmaMarker, Wide Range (6.5-205 kDa)
(Product Number M 4038)
Procedure
ColorBurst Markers come
TSPAN8 (7103)
Immunogen: Recombinant protein Transmembrane 4
superfamily member 3
Immunogen Sequence: GI # 4759238, sequence 110 - 205
The product is a clear, colorless solution in phosphate
buffered
5999)
Immunogen: Recombinant protein Regulator of G-protein
signaling 4
Immunogen Sequence: GI # 5032039, sequence 127 - 205
The product is a clear, colorless solution in phosphate
buffered saline
SPECIFICITY: LDL Receptor
IMMUNOGEN: Human LDL receptor, amino acids 29-205.
APPLICATIONS: Western blot: 1:500-1:2,000 (colormetric)
It is expected that the antibody will also work for immunocytochemistry
) is developed in
rabbit using as immunogen a synthetic peptide
corresponding to amino acids 186-205 located at
the C-terminus of human HSP27, conjugated to
KLH. This sequence is highly homologous in
concentration with Coomassie Bradford assays or 1:4 for BCA assays.
C. Preparation of lyophilized HeLa Cell Lysate: Unstimulated (Catalog # 47-205)
ASSAY CHARACTERISTICS (continued)
Specificity
2. Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
24152), PTV
injector, FID, and a dedicated data system. Temperature programming goes from 170 to 205 °C at the same
rate. Peaks are identified by the use of pure reference compounds.
Reference
2. Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
2. Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
2. Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
2. Kędzior, M. et al., Med. Microbiol. Immunol.,
205(4), 275-279 (2016).
3. Aoyagi, T. et al., J. Antibiot. (Tokyo), 22(6),
283-286 (1969).
4. Zimmerman, U-J.P. and Schlaepfer, W.W.,
Biochemistry
(1984).
4. Pellet cells by centrifugation at 500 x G, for 10 minutes.
5. Remove supernatant by careful aspiration.
6. Resuspend cells in 2 ml diluent.
7. Repeat washing procedure (steps 4-6) twice.
3
4
5
6
https://www.sigmaaldrich.com
4. Supplementary Information
y Version: 205-Bromo-2’-deoxy-uridine Labeling and Detection Kit II
sigma-aldrich.com 11
4. Supplementary
) on a 4-20% gradient gel
in the Laemmli system. Molecular weights determined
in this manner are available on lot specific Certificates
of Analysis.
Each vial of Color Markers contains 500 µl of
) on a 4-20% gradient gel
in the Laemmli system. Molecular weights determined
in this manner are available on lot specific Certificates
of Analysis.
Each vial of Color Markers contains 500 µl of
Anti-CAD is produced in rabbit using as immunogen a
synthetic peptide corresponding to amino acids
205-222 of mouse CAD (caspase-activated DNase)1.
Anti-CAD recognizes mouse CAD by immunoblotting
40
Preparation of lyophilized MILLIPLEX® MAP Cell Lysate (Catalog # 47-205).
MILLIPLEX® MAP HeLa Cell Lysate: Unstimulated (Catalog # 47-205) is provided
as a lyophilized stock of cell lysate prepared from