the pET System Manual (Technical Bulletin
055) should be followed. The final pellet from the osmotic shock procedure can then be used in
this protocol.
1. Harvest cells from liquid culture by centrifugation
Trinitrophenyl-lipopolysaccharide from Escherichia coli 055:B5 (T6682) - Product Information Sheet
Trinitrophenyl-lipopolysaccharide
from Escherichia coli 055:B5
Product Number T 6682
Storage Temperature