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Loredana Chierchia et al.
Zygote (Cambridge, England), 23(5), 669-682 (2014-09-18)
Actin-based cytoskeleton (CSK) and microtubules may bind to RNAs and related molecules implicated in translation. However, many questions remain to be answered regarding the role of cytoskeletal components in supporting the proteins involved in steps in the maturation and translation...
Joyce C Mello et al.
Molecules (Basel, Switzerland), 20(10), 17747-17759 (2015-09-26)
Plantago major L. produces several chemical substances with anti-inflammatory and analgesic activities and its use in the treatment of oral and throat inflammation in popular medicine is well described. In this study, the antioxidant potential of the Plantago major hydroethanolic...
Joanna Urban-Ciecko et al.
Current biology : CB, 25(6), 722-731 (2015-03-03)
Understanding the dynamic range for excitatory transmission is a critical component of building a functional circuit diagram for the mammalian brain. Excitatory synaptic transmission is typically studied under optimized conditions, when background activity in the network is low. The range...
Shiri Li et al.
Cell transplantation, 24(11), 2273-2283 (2015-01-13)
Oxidative stress is a major cause of islet damage and loss during the islet isolation process. The Nrf2 pathway plays a critical role in protecting the cells against oxidative stress. The aim of this study was to investigate the effect...
Sara Gosso et al.
The Journal of physiology, 592(15), 3215-3230 (2014-06-01)
Here we describe the ability of a high-density diamond microelectrode array targeted to resolve multi-site detection of fast exocytotic events from single cells. The array consists of nine boron-doped nanocrystalline diamond ultra-microelectrodes (9-Ch NCD-UMEA) radially distributed within a circular area...
Andrew S Torres et al.
The journal of trauma and acute care surgery, 77(3 Suppl 2), S94-S100 (2014-08-28)
Autologous platelet gel therapy using platelet-rich plasma has emerged as a promising alternative for chronic wound healing, hemostasis, and wound infection control. A critical step for this therapeutic approach is platelet activation, typically performed using bovine thrombin (BT) and calcium...
Gareth W Fearnley et al.
Biology open, 4(6), 731-742 (2015-04-26)
Vascular endothelial growth factor A (VEGF-A) regulates many aspects of vascular physiology such as cell migration, proliferation, tubulogenesis and cell-cell interactions. Numerous isoforms of VEGF-A exist but their physiological significance is unclear. Here we evaluated two different VEGF-A isoforms and...
Robin M Skory et al.
Human reproduction (Oxford, England), 30(6), 1386-1395 (2015-03-19)
Can cultured follicles model the ovarian cycle, including follicular- and luteal-phase hormone synthesis patterns and ovulation? Under gonadotrophin stimulation, murine follicles grown in an encapsulated three-dimensional system ovulate in vitro and murine and human follicle hormone synthesis mimics follicular and...
E Bezati et al.
Lupus, 24(1), 32-41 (2014-08-21)
Quinine, a quinoline derivative, is an ancient antipyretic drug with antimalarial properties that has been phased out by more effective synthetic candidates. In previous studies we discovered that hydroxychloroquine (HCQ), a synthetic antimalarial with structural similarities to quinine, reduced the...
Priscila Tomie Leme Ike et al.
SpringerPlus, 4, 654-654 (2015-11-07)
In this work we have identified, using mass spectrometry, two laccases produced by Leucoagaricus gongylophorus. One of them, Lac1Lg, was isolated, purified and characterized. Lac1Lg, a monomeric enzyme, was studied using ABTS and syringaldazine substrates. Lac1Lg presented kcat/Km almost threefold...
Tauseef M Asmat et al.
PloS one, 9(12), e114474-e114474 (2014-12-03)
The pili and outer membrane proteins of Neisseria meningitidis (meningococci) facilitate bacterial adhesion and invasion into host cells. In this context expression of meningococcal PilC1 protein has been reported to play a crucial role. Intracellular calcium mobilization has been implicated...
Rama D Yammani et al.
Journal of immunology (Baltimore, Md. : 1950), 193(11), 5434-5443 (2014-10-24)
Reductions in C4 levels may predispose individuals to infection with encapsulated bacteria as well as autoimmunity. In this study, we examined the role C4 has in protection against Streptococcus pneumoniae-induced autoimmunity. Mild respiratory infection with serotype 19F pneumococci selectively induced...
Åsa M E Winther et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(44), 14756-14770 (2015-11-06)
Many endocytic proteins accumulate in the reserve pool of synaptic vesicles (SVs) in synapses and relocalize to the endocytic periactive zone during neurotransmitter release. Currently little is known about their functions outside the periactive zone. Here we show that in...
Gui-lai Zuo et al.
PloS one, 10(3), e0121266-e0121266 (2015-03-26)
The hypoxia-inducible factors (HIFs), HIF-1α and HIF-2α, are the central mediators of the homeostatic response that enables cells to survive and differentiate in low-oxygen conditions. Previous studies indicated that disruption of the von Hippel-Lindau gene (Vhl) coincides with the activation...
Guolin Ma et al.
Nature communications, 6, 7826-7826 (2015-07-18)
Store-operated Ca(2+) entry mediated by STIM1 and ORAI1 constitutes one of the major Ca(2+) entry routes in mammalian cells. The molecular choreography of STIM1-ORAI1 coupling is initiated by endoplasmic reticulum (ER) Ca(2+) store depletion with subsequent oligomerization of the STIM1...
Hua Tian et al.
Journal of lipid research, 55(9), 1970-1980 (2014-05-24)
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with subcellular lateral resolution (∼4 μm). The method...
Lakhan Kma et al.
Asian Pacific journal of cancer prevention : APJCP, 15(15), 6429-6436 (2014-08-16)
Poly-ADP-ribosylation (PAR) is a post-translational modification of mainly chromosomal proteins. It is known to be strongly involved in several molecular events, including nucleosome-remodelling and carcinogenesis. In this investigation, it was attempted to evaluate PAR level as a reliable biomarker for...
Kenji Fukui et al.
The Journal of biological chemistry, 290(44), 26383-26392 (2015-09-16)
Diabetes mellitus is associated with a variety of complications, including alterations in the central nervous system (CNS). We have recently shown that diabetes results in a reduction of cholesterol synthesis in the brain due to decreased insulin stimulation of SREBP2-mediated...
Siddhartha Sankar Boxi et al.
Dalton transactions (Cambridge, England : 2003), 44(47), 20464-20474 (2015-11-07)
Arsenic poisoning from drinking water has been an important global issue in recent years. Because of the high level toxicity of arsenic to human health, an easy, inexpensive, low level and highly selective detection technique is of great importance to...
Dave J Koss et al.
Cellular and molecular life sciences : CMLS, 72(23), 4613-4632 (2015-06-14)
Patients suffering from tauopathies including frontotemporal dementia (FTD) and Alzheimer's disease (AD) present with intra-neuronal aggregation of microtubule-associated protein Tau. During the disease process, Tau undergoes excessive phosphorylation, dissociates from microtubules and aggregates into insoluble neurofibrillary tangles (NFTs), accumulating in...
Hannah H Lee et al.
Molecular cancer therapeutics, 14(4), 994-1003 (2015-02-20)
The antimicrotubular agent docetaxel is a widely used chemotherapeutic drug for the treatment of multiple solid tumors and is predominantly dependent on hepatic disposition. In this study, we evaluated drug uptake transporters capable of transporting radiolabeled docetaxel. By screening an...
A Iglesias-Ara et al.
Cell death and differentiation, 22(10), 1577-1589 (2015-02-07)
Tissue homeostasis requires tight regulation of cellular proliferation, differentiation and apoptosis. E2F1 and E2F2 transcription factors share a critical role in tissue homeostasis, since their combined inactivation results in overall organ involution, specially affecting the pancreatic gland, which subsequently triggers...
Davi A G Mázala et al.
American journal of physiology. Cell physiology, 308(9), C699-C709 (2015-02-06)
Duchenne muscular dystrophy (DMD) is characterized by progressive muscle wasting secondary to repeated muscle damage and inadequate repair. Elevations in intracellular free Ca²⁺ have been implicated in disease progression, and sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase 1 (SERCA1) overexpression has been shown to...
Tasha M Santiago-Rodriguez et al.
Microbiome, 3, 58-58 (2015-11-10)
Most human microbiota studies focus on bacteria inhabiting body surfaces, but these surfaces also are home to large populations of viruses. Many are bacteriophages, and their role in driving bacterial diversity is difficult to decipher without the use of in...
Kelly B Clancy et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(9), 3946-3958 (2015-03-06)
Layer (L)2 is a major output of primary sensory cortex that exhibits very sparse spiking, but the structure of sensory representation in L2 is not well understood. We combined two-photon calcium imaging with deflection of many whiskers to map whisker...
Andrew T Lucas et al.
PloS one, 9(11), e112823-e112823 (2014-11-15)
The translationally-controlled tumor protein (TCTP) is a highly conserved, ubiquitously expressed, abundant protein that is broadly distributed among eukaryotes. Its biological function spans numerous cellular processes ranging from regulation of the cell cycle and microtubule stabilization to cell growth, transformation...
Simona Buracco et al.
Journal of cell science, 128(17), 3304-3316 (2015-07-26)
The Nramp (Slc11) protein family is widespread in bacteria and eukaryotes, and mediates transport of divalent metals across cellular membranes. The social amoeba Dictyostelium discoideum has two Nramp proteins. Nramp1, like its mammalian ortholog (SLC11A1), is recruited to phagosomal and...
Morten Frier Gjerstorff et al.
Nucleic acids research, 42(18), 11433-11446 (2014-09-25)
Polycomb group (PcG) complexes regulate cellular identity through epigenetic programming of chromatin. Here, we show that SSX2, a germline-specific protein ectopically expressed in melanoma and other types of human cancers, is a chromatin-associated protein that antagonizes BMI1 and EZH2 PcG...
Ao Li et al.
Journal of virology, 89(15), 7646-7659 (2015-05-15)
Many viruses utilize viral or cellular chromatin machinery for efficient infection. Baculoviruses encode a conserved protamine-like protein, P6.9. This protein plays essential roles in various viral physiological processes during infection. However, the mechanism by which P6.9 regulates transcription remains unknown....
Michiko Nakamura et al.
Progress in neuro-psychopharmacology & biological psychiatry, 64, 35-43 (2015-07-16)
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the treatment of inflammatory pain. It is well established that NSAIDs exert their analgesic effects by inhibiting cyclooxygenase to prevent the production of prostaglandins; however, several NSAIDs including diclofenac also modulate other...