73-80
(1996).
7. Aigner, T. et al., Histochem. Cell Biol., 107, 435-40
(1997).
8. Girkontaite, I. et al., Matrix Biol., 15, 231-8 (1996).
9. Yamaguchi, N. et al., J. Biol. Chem., 264, 16022-
J. Neuroscience Methods 107: 131-140.
Draberova, E. et al. (1998). Expression of class III beta-tubulin in normal and neoplastic human
tissues. Histochem. Cell Biol. 109: 231-239.
Important
, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
PBS, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
, pH 7.4.
10. 2% paraformaldehyde in PBS.
11. Whole blood lysing solution.
12. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
1. Pellet 2 × 107 cells by centrifugation at
600 g for 10 minutes.
2. Carefully remove and discard the
supernatant.
Tissues
1. Isolate the tissue of interest (~100 mg).
2. Immerse the
PBS, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
Store all reagents at 2 – 8°C
Reagents Supplied
Catalog
Number
Volume Quantity
HepG2 Cell Lysate Control 47-231 lyophilized 1 vial
Set of one 96-Well Plate with 2
sealers
--------
Olson,
B. J., Klenk, D. C. (1985) Anal.
Biochem. 150, 76–85.
2. Wiechelman, K., Braun, R., and
Fitzpatrick, J. (1988) Anal. Biochem.
175, 231–237.
3. Hinson, D. L. and Webber, R. J.
(1988) BioTechniques
132 Caspase 13 (ERICE) C8854 8628 CASP13 P y y y
15 AKR1C3 A6229 8644 AKR1C3 M NP_003730.4 y n/d n/d
231 DR3 D3563 8718 TNFRSF25 P NP_001034753.1 y n/d n/d
644 RIP (Receptor Interacting Protein) R8274 8737
132 Caspase 13 (ERICE) C8854 8628 CASP13 P y y y
15 AKR1C3 A6229 8644 AKR1C3 M NP_003730.4 y n/d n/d
231 DR3 D3563 8718 TNFRSF25 P NP_001034753.1 y n/d n/d
644 RIP (Receptor Interacting Protein) R8274 8737
267 ) F8926 P y y n/d
307 Glycogen Synthase G6414 M y y y
230 DP2 D7438 M y n/d n/d
231 DR3 D3563 P y n/d n/d
232 Negative Control NA
233 DR4 D3813 P y n/d n/d
234