Chemistry (Weinheim an der Bergstrasse, Germany), 16(1), 158-166 (2009-12-02)
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been
Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay.
We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require
Spatial organization of proteins in metastasizing cells.
Ronnlund, D.; et al.
Cytometry, 83(9), 855-865 (2013)
STED microscopy resolves nanoparticle assemblies.
Willig K.I.; et al.
New Journal of Physics, 8(6), 106-106 (2006)
Block Copolymer Nanostructures Mapped by Far-Field Optics.
Ullal, C.K.; et al
Nano Letters, 9(6), 2497-2500 (2009)
Frequency dependent detection in a STED microscope using modulated excitation light.
Fluorescence resonance energy transfer (FRET) involving a semiconductor nanoparticle (NP) acting as a donor, attached to multiple acceptors, is becoming a common tool for sensing, biolabeling, and energy transfer applications. Such nanosystems, with dimensions that are in the range of
Experimental Proof of Concept of Nanoparticle-Assisted STED.
Sonnefraud, Y.; et al
Nano Letters, 14(8), 4449-4453 (2014)
A novel nanoscopic tool by combining AFM with STED microscopy.
Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers.
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Steshenko, O.; et al.
Biophysical Journal, 110(11), 2441-2450 (2016)
Size-Dependent Localization and Quantitative Evaluation of the Intracellular Migration of Silica Nanoparticles in Caco-2 Cells.
Schubbe, S.; et al.
Chemistry of Materials, 24(5), 914-923 (2012)
2PE-STED Microscopy with a Single Ti. Sapphire Laser for Reduced Illumination.
Twisted intercalating nucleic acid (TINA) is a novel intercalator and stabilizer of Hoogsteen type parallel triplex formations (PT). Specific design rules for position of TINA in triplex forming oligonucleotides (TFOs) have not previously been presented. We describe a complete collection
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction
Subunit rotation in a single F0F1-ATP synthase in a living bacterium monitored by FRET.
Seyfert, K.,et al.
Proceedings of SPIE, 7905, 79050K-79050K (2011)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)
Maturation of active zone assembly by Drosophila Bruchpilot.
Fouquet, W.; et al.
The Journal of Cell Biology, 186(1), 129-145 (2009)
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
Heller, I.; et al.
Nature Methods, 10(9), 910-916 (2013)
Two-photon excitation STED microscopy
Moneron, G.; Hell, S. W.
Optics Express, 17(17), 14567-14573 (2009)
Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach, M. A.; et al.
Journal of Biophotonics, 3(7), 417-424 (2010)
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig K.I.; et al.
Nature, 440(7086), 935-939 (2006)
Sub-diffraction imaging of nitrogen-vacancy centers in diamond by stimulated emission depletion and structured illumination
Xusan, Y., et al.
Royal Society of Chemistry Advances, 4(22), 11305-11305 (2014)
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