stability of productivity, selectivity and sterility.
Inoculation was carried out at 0, 3, 6, 9, 24, 48, 72 and
96 hours after media preparation. Using sterile water
(autoclaved), prepared Readybag®
ladder 1 2
24 nt
17 bp
21 bp
25 bp
Native PAGE, 12%
48 nt ssRNA
48 bp dsRNA
Lane 1: 48 bp dsRNA,
TENPORA™
Lane 2: 48 nt oligonucleotide,
synthesized by TC
Plate size Cells per well Surface area per well (cm2)† Volume per well (mL)
96-well 5.0 x 103 - 1.0 x 104 0.32 0.1
48-well 1.5 x 104 – 3.0 x 104 0.95 0.3
24-well 3.0 x
stock solution in PBS was transferred in a checkerboard pattern
to 48 of 96 wells of a MultiscreenHTS Solubility filter plate. The 48 adjacent wells received an equivalent volume of PBS. The filter plate
a white 96 well
plate according to Table 1.
Table 1.
Preparation of Cardiolipin Standards
Well
250 M
Premix
CL Assay
Buffer
Cardiolipin
(nmol/well)
1 0 L 50
and 5% CO2, for
24-48 hours.
4
Streamlined Method Protocol
Preparation of adherent cells
Using the appropriate antibiotic-free complete
medium, seed a sterile 96 well or alternate size
for configurable
30-plex) or
HCYTB-6060-2
(for 38-plex and
48-plex)
Serum Matrix Lyophilized 1 vial MXHSM-B
Set of one 96-Well Plate
with 2 sealers - 1 set -
Assay Buffer 30 mL
200 0.2 1
1 well of a 48 well plate 0.75 100 0.075 0.5
1 well of a 96 well plate 0.32 50 0.02 0.2–0.25
* ∼1 × 105 viable cells per cm2 of the fibronectin-coated cultureware
Day 0 –
200 0.2 1
1 well of a 48 well plate 0.75 100 0.075 0.5
1 well of a 96 well plate 0.32 50 0.02 0.2–0.25
* ∼1 × 105 viable cells per cm2 of the fibronectin-coated cultureware
Day 0 –
200 0.2 1
1 well of a 48 well plate 0.75 100 0.075 0.5
1 well of a 96 well plate 0.32 50 0.02 0.2–0.25
* ∼1 × 105 viable cells per cm2 of the fibronectin-coated cultureware
Day 0 –
200 0.2 1
1 well of a 48 well plate 0.75 100 0.075 0.5
1 well of a 96 well plate 0.32 50 0.02 0.2–0.25
* ∼1 × 105 viable cells per cm2 of the fibronectin-coated cultureware
Day 0 –
200 0.2 1
1 well of a 48 well plate 0.75 100 0.075 0.5
1 well of a 96 well plate 0.32 50 0.02 0.2–0.25
* ∼1 × 105 viable cells per cm2 of the fibronectin-coated cultureware
Day 0 –