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Zeus Saldaña et al.
PloS one, 9(7), e101200-e101200 (2014-07-19)
Uropathogenic Escherichia coli (UPEC) strains cause urinary tract infections and employ type 1 and P pili in colonization of the bladder and kidney, respectively. Most intestinal and extra-intestinal E. coli strains produce a pilus called E. coli common pilus (ECP)
Dorival Martins et al.
Frontiers in microbiology, 11, 576708-576708 (2020-10-27)
Stressors and environmental cues shape the physiological state of bacteria, and thus how they subsequently respond to antibiotic toxicity. To understand how superoxide stress can modulate survival to bactericidal antibiotics, we examined the effect of intracellular superoxide generators, paraquat and
Cláudia R Serra et al.
Scientific reports, 9(1), 6384-6384 (2019-04-24)
The gastrointestinal microbiota plays a critical role on host health and metabolism. This is particularly important in teleost nutrition, because fish do not possess some of the necessary enzymes to cope with the dietary challenges of aquaculture production. A main
Samira Boudebbouze et al.
Recent patents on DNA & gene sequences, 5(3), 194-201 (2011-08-27)
D-tagatose is a natural monosaccharide with a low caloric value and has an anti-hyperglycemiant effect. This hexose has potential applications both in pharmaceutical and agro-food industries. However, the use of D-tagatose remains limited by its production cost. Many production procedures
Cristina Manatschal et al.
eLife, 8 (2019-12-06)
In humans, the divalent metal ion transporter-1 (DMT1) mediates the transport of ferrous iron across the apical membrane of enterocytes. Hence, its inhibition could be beneficial for the treatment of iron overload disorders. Here we characterize the interaction of aromatic
Aman Kumar et al.
Cell host & microbe, 28(1), 41-53 (2020-06-11)
The gut-brain axis is crucial to microbial-host interactions. The neurotransmitter serotonin is primarily synthesized in the gastrointestinal (GI) tract, where it is secreted into the lumen and subsequently removed by the serotonin transporter, SERT. Here, we show that serotonin decreases
Dipal M Patel et al.
The Journal of cell biology, 206(6), 779-797 (2014-09-17)
Mechanisms by which microtubule plus ends interact with regions of cell-cell contact during tissue development and morphogenesis are not fully understood. We characterize a previously unreported interaction between the microtubule binding protein end-binding 1 (EB1) and the desmosomal protein desmoplakin
Young-Ho Hong et al.
Journal of agricultural and food chemistry, 59(24), 12939-12947 (2011-11-23)
Hyperthermophilic L-arabinose isomerases (AIs) are useful in the commercial production of D-tagatose as a low-calorie bulk sweetener. Their catalysis and thermostability are highly dependent on metals, which is a major drawback in food applications. To study the role of metal
James D Oliver et al.
Biotechnology and applied biochemistry, 62(3), 329-342 (2014-07-22)
Free-solution capillary electrophoresis (CE), or capillary zone electrophoresis, with direct UV detection was used for the first time for the determination of mono- and disaccharides, sugar alcohols, and ethanol in fermentation broths. Sample preparation proved to be minimal: no derivatization
Alfred Fillol-Salom et al.
Molecular cell, 75(5), 1020-1030 (2019-07-28)
Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and
Martín A Rossotti et al.
Analytical chemistry, 87(23), 11907-11914 (2015-11-07)
Single domain heavychain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. However, there is no simple method to select the most valuable nanobodies from the crowd of positive clones obtained after the initial screening.
Ahyun Son et al.
RNA biology, 12(11), 1198-1208 (2015-10-31)
It is one of the fundamental questions in biology how proteins efficiently fold into their native conformations despite off-pathway events such as misfolding and aggregation in living cells. Although molecular chaperones have been known to assist the de novo folding
Patricia Bonin et al.
Environmental science and pollution research international, 22(20), 15347-15359 (2015-01-07)
The impact of petroleum contamination and of burrowing macrofauna on abundances of Marinobacter and denitrifiers was tested in marine sediment mesocoms after 3 months incubation. Quantification of this genus by qPCR with a new primer set showed that the main
Sirinan Shompoosang et al.
Bioscience, biotechnology, and biochemistry, 78(2), 317-325 (2014-07-19)
6-Deoxy-L-glucose, 6-deoxy-L-altrose, and 6-deoxy-L-allose were produced from L-rhamnose with an immobilized enzyme that was partially purified (IE) and an immobilized Escherichia coli recombinant treated with toluene (TT). 6-Deoxy-L-psicose was produced from L-rhamnose by a combination of L-rhamnose isomerase (TT-PsLRhI) and
Ying Liu et al.
Bio-protocol, 8(5), e2753-e2753 (2018-03-05)
Cyclic di-guanosine monophosphate (c-di-GMP) is a ubiquitous second messenger that regulates distinct aspects of bacterial physiology. It is synthesized by diguanylate cyclases (DGCs) and hydrolyzed by phosphodiesterases (PDEs). To date, the activities of DGC and PDE are commonly assessed by
Fei Chen et al.
Bio-protocol, 8(12), e2893-e2893 (2018-06-20)
Control of bacterial adhesions to a substrate with high precision in space and time is important to form a well-defined biofilm. Here, we present a method to engineer bacteria such that they adhere specifically to substrates under blue light through
Yoshikiyo Sakakibara et al.
Journal of bioscience and bioengineering, 107(5), 506-511 (2009-04-28)
An Escherichia coli strain, ZUC99(pATX210), which can produce xylitol from L-arabinose at a high yield, has been created by introducing a new bioconversion pathway into the cells. This pathway consists of three enzymes: L-arabinose isomerase (which converts L-arabinose to L-ribulose)
David Rabuka et al.
Nature protocols, 7(6), 1052-1067 (2012-05-12)
We describe a method for modifying proteins site-specifically using a chemoenzymatic bioconjugation approach. Formylglycine generating enzyme (FGE) recognizes a pentapeptide consensus sequence, CxPxR, and it specifically oxidizes the cysteine in this sequence to an unusual aldehyde-bearing formylglyine. The FGE recognition
Chen Chen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 969, 171-180 (2014-09-01)
One challenge facing the purification of therapeutic glycoproteins by affinity chromatography is creating ligands specific for the glycan moiety. Affinity chromatography of glycoproteins is currently conducted with immobilized lectins or boronates, although biomimetic ligands could present a more desirable option.
Christopher S Frei et al.
Protein science : a publication of the Protein Society, 25(4), 804-814 (2016-01-11)
The Escherichia coli regulatory protein AraC regulates expression of ara genes in response to l-arabinose. In efforts to develop genetically encoded molecular reporters, we previously engineered an AraC variant that responds to the compound triacetic acid lactone (TAL). This variant
Yanpei Liu et al.
Plant physiology, 183(1), 194-205 (2020-03-12)
Photorespiration is an essential process in oxygenic photosynthetic organisms triggered by the oxygenase activity of Rubisco. In peroxisomes, photorespiratory HYDROXYPYRUVATE REDUCTASE1 (HPR1) catalyzes the conversion of hydroxypyruvate to glycerate together with the oxidation of a pyridine nucleotide cofactor. HPR1 regulation
Tania Chroumpi et al.
Frontiers in bioengineering and biotechnology, 9, 644216-644216 (2021-03-26)
The filamentous ascomycete Aspergillus niger has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses
Maiko Furubayashi et al.
Nature communications, 6, 7534-7534 (2015-07-15)
Synthetic biology aspires to construct natural and non-natural pathways to useful compounds. However, pathways that rely on multiple promiscuous enzymes may branch, which might preclude selective production of the target compound. Here, we describe the assembly of a six-enzyme pathway
Danielle Ahn et al.
Cell reports, 35(9), 109196-109196 (2021-06-03)
Klebsiella pneumoniae ST258 is a human pathogen associated with poor outcomes worldwide. We identify a member of the acyltransferase superfamily 3 (atf3), enriched within the ST258 clade, that provides a major competitive advantage for the proliferation of these organisms in vivo.
Jin Hur et al.
Veterinary immunology and immunopathology, 164(3-4), 101-109 (2015-02-26)
In this study, a comparative evaluation between a Salmonella ghost vaccine expressing enterotoxigenic Escherichia coli (ETEC) fimbrial antigens and a commercial ETEC vaccine was conducted in neonatal piglets. Genes encoding the ETEC K88ab, K88ac, K99, FasA, and F41 fimbrial proteins
Liao Chang et al.
PloS one, 11(12), e0168744-e0168744 (2016-12-21)
Whole-genome shotgun (WGS) sequencing has become a routine method in genome research over the past decade. However, the assembly of highly polymorphic regions in WGS projects remains a challenge, especially for large genomes. Employing BAC library constructing, PCR screening and
Jonghyeon Shin et al.
Molecular systems biology, 16(3), e9401-e9401 (2020-03-07)
Synthetic genetic circuits offer the potential to wield computational control over biology, but their complexity is limited by the accuracy of mathematical models. Here, we present advances that enable the complete encoding of an electronic chip in the DNA carried
Jiahui Wu et al.
Nature communications, 5, 5262-5262 (2014-11-02)
The introduction of calcium ion (Ca(2+)) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca(2+) dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared
Krešimir Šola et al.
The Plant cell, 31(4), 809-831 (2019-03-11)
Cell-to-cell adhesion is essential for establishment of multicellularity. In plants, such adhesion is mediated through a middle lamella composed primarily of pectic polysaccharides. The molecular interactions that influence cell-to-cell adhesion are not fully understood. We have used Arabidopsis (Arabidopsis thaliana)
Trevor Y H Ho et al.
Nature communications, 12(1), 2200-2200 (2021-04-15)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augment
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