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Larry H Stanker et al.
Methods in molecular biology (Clifton, N.J.), 1318, 69-78 (2015-07-15)
The sandwich immunoassay (sELISA) is an invaluable technique for concentrating, detecting, and quantifying target antigens. The two critical components required are a capture antibody and a detection antibody, each binding a different epitope on the target antigen. The specific antibodies
Bin Chen et al.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 31(3), 264-271 (2020-04-29)
To investigate the effect and the possible mechanism of lanthanum citrate on the proliferation and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 through the Hedgehog (Hh) signaling pathway. Different concentrations of lanthanum citrate and KAAD-cyclopamine (the Hh signaling
Jorge Dinamarca et al.
Journal of phycology, 53(2), 405-414 (2017-01-13)
Under nutrient deplete conditions, diatoms accumulate between 15% to 25% of their dry weight as lipids, primarily as triacylglycerols (TAGs). As in most eukaryotes, these organisms produce TAGs via the acyl-CoA dependent Kennedy pathway. The last step in this pathway
Shamroop K Mallela et al.
PloS one, 11(1), e0145831-e0145831 (2016-01-12)
Ceramide is synthesized in yeast by two redundant acyl-CoA dependent synthases, Lag1 and Lac1. In lag1∆ lac1∆ cells, free fatty acids and sphingoid bases are elevated, and ceramides are produced through the redundant alkaline ceramidases Ypc1 and Ydc1, working backwards.
Paige L Jany et al.
ASN neuro, 5(1), e00109-e00109 (2013-02-26)
AxD (Alexander disease) is a rare disorder caused by heterozygous mutations in GFAP (glial fibrillary acidic protein) resulting in accumulation of the GFAP protein and elevation of Gfap mRNA. To test whether GFAP itself can serve as a biomarker of
D J Schendel et al.
Gene therapy, 7(11), 950-959 (2000-06-13)
Even though renal cell carcinomas (RCC) are thought to be immunogenic, many tumors express variations in surface molecules and intracellular proteins that hinder induction of optimal antitumor responses. Interferon gamma (IFNgamma) stimulation can correct some of these deficiencies. Therefore, we
Prativa Majee et al.
Molecular therapy. Nucleic acids, 23, 691-701 (2021-02-13)
Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent
M Wilming et al.
FEBS letters, 509(2), 272-276 (2001-12-14)
The inter- and intramolecular interactions between the different domains of the catalase-peroxidase KatG from Mycobacterium tuberculosis were analyzed using the two-hybrid assay. It was shown that the dimerization of the enzyme is due to a strong interaction of the first
Flavia Chiuppesi et al.
Nature communications, 11(1), 6121-6121 (2020-12-02)
Modified Vaccinia Ankara (MVA) is a highly attenuated poxvirus vector that is widely used to develop vaccines for infectious diseases and cancer. We demonstrate the construction of a vaccine platform based on a unique three-plasmid system to efficiently generate recombinant
Rui Costa et al.
Journal of hepatology, 70(4), 603-614 (2018-11-26)
The lipid-binding protein, SEC14L2, is crucial for the efficient viral replication of clinical hepatitis C virus (HCV) isolates in cell culture. Given the role of SEC14L2 in HCV replication, we aimed to study a large number of HCV positive sera
Luisa F Bustamante-Jaramillo et al.
PLoS genetics, 15(1), e1007876-e1007876 (2019-01-15)
CDKs (cyclin-dependent kinases) associate with different cyclins to form different CDK-complexes that are fundamental for an ordered cell cycle progression, and the coordination of this progression with different aspects of the cellular physiology. During meiosis programmed DNA double-strand breaks (DSBs)
Carolina Gillio-Meina et al.
Reproduction (Cambridge, England), 137(3), 537-552 (2008-12-09)
To determine if changes in endometrial expression of the enzymes and receptors involved in prostaglandin (PG) synthesis and action might provide insights into the PGs involved in the initiation of decidualization, ovariectomized steroid-treated rats at the equivalent of day 5
Yuh-Hwa Liu et al.
Botanical studies, 60(1), 9-9 (2019-06-30)
There were few report concerning anti-glycation and antioxidant activities of the minor amounts of components in curcuminoids, demethylcurcumin and tetrahydroxycurcumin, in vitro and in vivo. The bovine serum albumin/galactose of non-enzymatic glycation models, radical-induced hemolysis, and oxygen radical absorbance capacity
S V Demyanenko et al.
Molecular neurobiology, 57(1), 226-238 (2019-09-08)
In ischemic stroke, vascular occlusion rapidly induces tissue infarct. Over the ensuing hours, damage spreads to adjacent tissue and forms transition zone (penumbra), which is potentially salvageable. Epigenetic regulation of chromatin structure controls gene expression and protein synthesis. We studied
Wanyun Li et al.
Journal of cell science, 134(8) (2021-03-25)
Autophagy is considered to be an important switch for facilitating normal to malignant cell transformation during colorectal cancer development. Consistent with other reports, we found that the membrane receptor Neuropilin1 (NRP1) is greatly upregulated in colon cancer cells that underwent
Mengqian Luo et al.
Bio-protocol, 8(12), e2880-e2880 (2018-06-20)
As a fundamental metabolic pathway to degrade and recycle cellular cargos, autophagy is highly induced upon stress, starvation and senescence conditions in plants. A double-membrane structure named autophagosome will form during this process for cargo sequestration and delivery into the
Damian M Janecki et al.
International journal of molecular sciences, 21(8) (2020-04-30)
While two mouse NANOS paralogues, NANOS2 and NANOS3, are crucial for maintenance of germ cells by suppression of apoptosis, the mouse NANOS1 paralogue does not seem to regulate these processes. Previously, we described a human NANOS1 p.[(Pro34Thr);(Ser83del)] mutation associated with
Kirsten Lindner et al.
International journal of molecular sciences, 19(2) (2018-02-08)
Resistance towards chemotherapy is a major obstacle in the treatment of esophageal squamous cell carcinoma (ESCC). We investigated the role of specific microRNAs in chemotherapy resistance and tumor biology. We selected three microRNAs from characteristic microRNA signatures of resistant ESCC
F Duclot et al.
Neuroscience, 167(3), 850-863 (2010-03-12)
p300/CREB binding protein-associated factor (PCAF) regulates gene expression by acting through histone acetylation and as a transcription coactivator. Although histone acetyltransferases were involved in the toxicity induced by amyloid-beta (Abeta) peptides, nothing is known about PCAF. We here analyzed the
Zunlong Ke et al.
Nature, 588(7838), 498-502 (2020-08-18)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions are surrounded by a lipid bilayer from which spike (S) protein trimers protrude1. Heavily glycosylated S trimers bind to the angiotensin-converting enzyme 2 receptor and mediate entry of virions into target cells2-6. S
Patrick Eldin et al.
Nucleic acids research, 42(3), 1698-1710 (2013-11-02)
The human immunodeficiency virus type 1 (HIV-1) Vpr protein binds to the cellular uracil-DNA glycosylase UNG2 and induces its degradation through the assembly with the DDB1-CUL4 ubiquitin ligase complex. This interaction counteracts the antiviral activity exerted by UNG2 on HIV-1
Pablo Perez-Pinera et al.
Nature methods, 10(3), 239-242 (2013-02-05)
Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with
Anshuman Das et al.
Journal of virology, 88(6), 3103-3113 (2013-12-29)
In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) as a factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand the
Anindya Dey et al.
Molecular cancer therapeutics, 17(1), 39-49 (2017-11-22)
BMI-1, also known as a stem cell factor, is frequently upregulated in several malignancies. Elevated expression of BMI-1 correlates with poor prognosis and is therefore considered a viable therapeutic target in a number of malignancies including ovarian cancer. Realizing the
Tracy L Hagemann et al.
PloS one, 7(5), e37304-e37304 (2012-06-14)
In Alexander disease (AxD) the presence of mutant glial fibrillary acidic protein (GFAP), the major intermediate filament of astrocytes, triggers protein aggregation, with marked induction of a stress response mediated by the transcription factor, Nrf2. To clarify the role of
Sandra V Ashton et al.
Arteriosclerosis, thrombosis, and vascular biology, 25(1), 102-108 (2004-10-23)
Invasion of uterine spiral arteries by extravillous trophoblasts in the first trimester of pregnancy results in loss of endothelial and musculoelastic layers. This remodeling is crucial for an adequate blood supply to the fetus with a failure to remodel implicated
Manuel Giménez-Andrés et al.
eLife, 10 (2021-04-16)
Numerous proteins target lipid droplets (LDs) through amphipathic helices (AHs). It is generally assumed that AHs insert bulky hydrophobic residues in packing defects at the LD surface. However, this model does not explain the targeting of perilipins, the most abundant
Qi Liu et al.
Nature communications, 11(1), 4857-4857 (2020-09-27)
Characterization of the dynamic conformational changes in membrane protein signaling complexes by nuclear magnetic resonance (NMR) spectroscopy remains challenging. Here we report the site-specific incorporation of 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code expansion. Crystallographic analysis revealed structural changes
Monica Boffito et al.
PloS one, 13(7), e0199896-e0199896 (2018-07-07)
Scaffolds populated with human cardiac progenitor cells (CPCs) represent a therapeutic opportunity for heart regeneration after myocardial infarction. In this work, square-grid scaffolds are prepared by melt-extrusion additive manufacturing from a polyurethane (PU), further subjected to plasma treatment for acrylic
Emma Branigan et al.
Nature communications, 11(1), 2846-2846 (2020-06-07)
Based on extensive structural analysis it was proposed that RING E3 ligases prime the E2~ubiquitin conjugate (E2~Ub) for catalysis by locking it into a closed conformation, where ubiquitin is folded back onto the E2 exposing the restrained thioester bond to
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