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B2645

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Jiannan Sun et al.
Journal of chromatography. A, 1379, 112-117 (2015-01-13)
This study achieved resolution improvement for iodine speciation in the presence of an ion-pairing reagent by a pressure-driven capillary electrophoresis (CE) system. Addition of 0.01mM tetrabutyl ammonium hydroxide (TBAH) as the ion-pairing reagent into the electrophoretic buffer resulted in the
Dana M Lord et al.
Biochemistry, 53(46), 7223-7231 (2014-11-08)
MqsR-controlled colanic acid and biofilm regulator (McbR, also known as YncC) is the protein product of a highly induced gene in early Escherichia coli biofilm development and has been regarded as an attractive target for blocking biofilm formation. This protein
Dan Shu et al.
Nanomedicine (London, England), 10(12), 1881-1897 (2015-07-04)
To find methods for potent drug development by targeting to biocomplex with high copy number. Phi29 DNA packaging motor components with different stoichiometries were used as model to assay virion assembly with Yang Hui's Triangle [Formula: see text], where Z
Hye Ryeo Lee et al.
Journal of chromatography. A, 1346, 117-122 (2014-05-09)
In liquid phase microextraction, high enrichment factors can be obtained using an acceptor phase of small volume. By hanging an acceptor drop at the separation capillary tip, single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis (CE). The
Biebele Abel et al.
Journal of immunological methods, 411, 43-49 (2014-06-21)
In this study, we report the use of an enzyme-based hybrid platform, which is comprised of silver island films, enzymes (HRP and AP) and high-throughput screening (HTS) microplates, to enhance the colorimetric response of enzymatic reactions. The hybrid platform was
Mathieu Rajalu et al.
Neuropharmacology, 88, 145-154 (2014-09-10)
GABAB receptors (GABABRs) are considered promising drug targets for the treatment of mental health disorders. GABABRs are obligate heteromers of principal GABAB1 and GABAB2 subunits. GABABRs can additionally associate with auxiliary KCTD8, 12, 12b and 16 subunits, which also bind
Blanca V Rodriguez et al.
Analytical biochemistry, 492, 69-75 (2015-09-30)
High-quality chromosomal DNA is a requirement for many biochemical and molecular biological techniques. To isolate cellular DNA, standard protocols typically lyse cells and separate nucleic acids from other biological molecules using a combination of chemical and physical methods. After a
Francesca Cima et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 169, 16-24 (2015-01-13)
New biocides such as the organometallic compound zinc pyrithione (ZnP) have been massively introduced by many countries in formulations of antifouling paints following the ban on tributyltin (TBT). The effects of sublethal concentrations (LC50=82.5 μM, i.e., 26.2 mg/l) on cultured
Ying Rang et al.
Journal of separation science, 38(15), 2722-2728 (2015-05-27)
A quantitative sample introduction method based upon inkjet injection was applied to capillary electrophoresis coupled with stacking and sweeping on-line concentration techniques. Methylxanthines were used as model compounds for the proof-of-concept of the method. The volume of injected sample could
Lisa Mathiasen et al.
PloS one, 10(4), e0125789-e0125789 (2015-04-10)
Human PREP1 and PBX1 are homeodomain transcriptional factors, whose biochemical and structural characterization has not yet been fully described. Expression of full-length recombinant PREP1 (47.6 kDa) and PBX1 (46.6 kDa) in E. coli is difficult because of poor yield, high
Ramprasad Kandavar et al.
Journal of clinical hypertension (Greenwich, Conn.), 17(1), 14-19 (2014-12-17)
Prehypertension is an important phenotype for cardiovascular risk and development of established hypertension. To better understand the early circulatory changes in this group, the authors studied 34 patients with prehypertension (blood pressure 120-139/80-89 mm Hg) using digital plethysmography for measurement
J L Ordóñez et al.
Food chemistry, 178, 221-228 (2015-02-24)
This paper studies the amino acid profile of beverages obtained through the fermentation of strawberry purée by a surface culture using three strains belonging to different acetic acid bacteria species (one of Gluconobacter japonicus, one of Gluconobacter oxydans and one
Zhi-Zhun Mo et al.
BMC complementary and alternative medicine, 15, 238-238 (2015-07-17)
The inhibitory effect of andrographolide sodium bisulphite (ASB) on jack bean urease (JBU) and Helicobacter pylori urease (HPU) was performed to elucidate the inhibitory potency, kinetics and mechanism of inhibition in 20 mM phosphate buffer, pH 7.0, 2 mM EDTA
P A Mosier-Boss et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 153, 591-598 (2015-10-07)
SERS substrates were fabricated by filtering either Ag or Au colloidal particles onto rigid, ceramic filters - onto which suspensions of bacteria were then filtered. SERS spectra of the bacteria were obtained using a Raman spectrometer that has an 'orbital
Sonika Batra et al.
Biomedical chromatography : BMC, 28(6), 815-825 (2014-05-28)
Enantiomeric separation of racemic mexiletine and fluoxetine was achieved using three chiral derivatizing reagents (CDRs) based on (S)-naproxen. Diastereomers were synthesized by reaction of mexiletine or fluoxetine with the CDRs and were separated on a C18 column under reversed-phase conditions
M Amirul Alam et al.
Comptes rendus biologies, 338(1), 1-11 (2014-12-04)
Genetic diversity and relationships among 45 collected purslane accessions were evaluated using ISSR markers. The 28 primers gave a total of 167 bands, among which 163 were polymorphic (97.6%). The genetic diversity as estimated by Shannon's information index was 0.513
Azizollah Nezhadali et al.
Talanta, 146, 525-532 (2015-12-24)
The present work describes development of a simple and cost-effective electrochemical sensor for determination of 1,4-dihydroxyanthraquinone (1,4-DAQ) based on molecularly imprinted polypyrrole (PPY). The molecularly imprinted polymer (MIP) was electrochemically synthesized onto surface of multi-walled carbon-nanotubes-modified pencil graphite electrode (MWCNs-PGE).
Teresa Balbi et al.
The Science of the total environment, 493, 355-364 (2014-06-22)
The increasing production of nanoparticles (NPs) will lead to their release into the aquatic environment, where they could modify the bioavailability/bioconcentration and consequent biological impact of other contaminants. Interactive effects of n-TiO2, one of the most widespread NP type, and
Juan Peris-Vicente et al.
Journal of chromatographic science, 52(9), 1121-1126 (2013-11-14)
A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and
Xianxie Zhang et al.
Drug design, development and therapy, 9, 6343-6362 (2015-12-18)
Tanshinone IIA (Tan IIA) (C19H18O3) is one of the major active lipophilic components in a conventional Chinese medicine called danshen, and it has long been used in the People's Republic of China and other neighboring countries to treat patients suffering
Javad Tashkhourian et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 152, 119-125 (2015-07-24)
A rapid, simple and inexpensive spectrofluorimetric sensor for determination of doxycycline based on its interaction with thioglycolic acid-capped cadmium telluride quantum dots (TGA/CdTe QDs) has been developed. Under the optimum experimental conditions, the sensor exhibited a fast response time of
Sofia Henriksson et al.
Genes & development, 28(24), 2726-2738 (2014-12-17)
The WD40 domain-containing protein WRAP53β (WD40 encoding RNA antisense to p53; also referred to as WDR79/TCAB1) controls trafficking of splicing factors and the telomerase enzyme to Cajal bodies, and its functional loss has been linked to carcinogenesis, premature aging, and
Ruchira N Wijesena et al.
Carbohydrate polymers, 109, 56-63 (2014-05-13)
Chitin nanofibers have been prepared from crab shell as a chitin source using ultrasound assisted fibrillation. Atomic force microscopy (AFM) study showed that the prepared nanofibers were having diameters and lengths primarily in the range of 2-20 nm and 0.3-4
Ana C Moreira et al.
Biochimica et biophysica acta, 1842(12 Pt A), 2468-2478 (2014-10-07)
The cardiotoxicity induced by the anti-cancer doxorubicin involves increased oxidative stress, disruption of calcium homeostasis and activation of cardiomyocyte death. Nevertheless, antioxidants and caspase inhibitors often show little efficacy in preventing cell death. We hypothesize that a caspase-independent cell death
Emil Meng-Lund et al.
Journal of pharmaceutical sciences, 103(7), 2053-2061 (2014-05-16)
The pH partition theory proposes a correlation between fraction of unionized drug substance and permeability. The aim of this study was to compare the permeability of metoprolol and mannitol in ex vivo human and porcine buccal mucosa models at varying
Yiting Wang et al.
British journal of pharmacology, 171(19), 4490-4503 (2014-06-07)
Dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel causes the genetic disease cystic fibrosis (CF). Towards the development of transformational drug therapies for CF, we investigated the channel function and action of CFTR potentiators on A561E, a
Tiffany C Delport et al.
Journal of wildlife diseases, 51(3), 555-563 (2015-04-29)
Greater interaction between humans and wildlife populations poses significant risks of anthropogenic impact to natural ecosystems, especially in the marine environment. Understanding the spread of microorganisms at the marine interface is therefore important if we are to mitigate adverse effects
Yves Claude Guillaume et al.
Biomedical chromatography : BMC, 29(9), 1338-1342 (2015-02-04)
In a previous paper Guillaume's group demonstrated that magnesium (Mg(2+) concentration range 0.00-2.60 mm) increased the passive diffusion of statins and thus played a role in their potential toxicity. In order to confirm an increase in this passive diffusion by
Alessia Finotti et al.
Pharmacological research, 91, 57-68 (2014-12-06)
Rapamycin, an inhibitor of mTOR activity, is a potent inducer of erythroid differentiation and fetal hemoglobin production in β-thalassemic patients. Mithramycin (MTH) was studied to see if this inducer of K562 differentiation also operates through inhibition of mTOR. We can
Luis Alberto Luévano-Martínez et al.
Biochimica et biophysica acta, 1847(6-7), 587-598 (2015-04-07)
Mitochondria play a key role in adaptation during stressing situations. Cardiolipin, the main anionic phospholipid in mitochondrial membranes, is expected to be a determinant in this adaptive mechanism since it modulates the activity of most membrane proteins. Here, we used
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