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Claire Vinel et al.
Nature communications, 12(1), 6130-6130 (2021-10-23)
Epigenetic mechanisms which play an essential role in normal developmental processes, such as self-renewal and fate specification of neural stem cells (NSC) are also responsible for some of the changes in the glioblastoma (GBM) genome. Here we develop a strategy
L P Thuy et al.
Clinica chimica acta; international journal of clinical chemistry, 202(3), 191-197 (1991-10-31)
A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose.
Xiaoxue Zhou et al.
eLife, 10 (2021-01-23)
In budding yeast, the mitotic exit network (MEN), a GTPase signaling cascade, integrates spatial and temporal cues to promote exit from mitosis. This signal integration requires transmission of a signal generated on the cytoplasmic face of spindle pole bodies (SPBs;
P Prandoni et al.
Minerva medica, 104(2), 131-139 (2013-03-22)
In recent years, the significant limitations associated with the use of vitamin K antagonists (VKA) have encouraged the development of new agents. Based upon the central roles played by the serine proteases thrombin and Factor Xa in the blood coagulation
L P Thuy et al.
Prenatal diagnosis, 19(2), 108-112 (1999-04-24)
Holocarboxylase synthetase is one of two enzymes known to be involved in the metabolism of biotin. It catalyses the fixation of biotin to inactive apocarboxylases yielding active carboxylases. Deficiency of this enzyme leads to multiple carboxylase deficiency which is fatal
Elena de Juan-Franco et al.
Talanta, 114, 268-275 (2013-08-21)
We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-antibody sensor surface specific for both hormone
Dries A M Feyen et al.
Cell reports, 32(3), 107925-107925 (2020-07-23)
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have enormous potential for the study of human cardiac disorders. However, their physiological immaturity severely limits their utility as a model system and their adoption for drug discovery. Here, we describe maturation media designed
Steven Lin et al.
Molecular bioSystems, 7(6), 1811-1821 (2011-03-26)
Biotin is an enzyme cofactor indispensable to metabolic fixation of carbon dioxide in all three domains of life. Although the catalytic and physiological roles of biotin have been well characterized, the biosynthesis of biotin remains to be fully elucidated. Studies
Heejin Jun et al.
Developmental cell, 54(1), 106-116 (2020-06-14)
Maintaining energy homeostasis upon environmental challenges, such as cold or excess calorie intake, is essential to the fitness and survival of mammals. Drug discovery efforts targeting β-adrenergic signaling have not been fruitful after decades of intensive research. We recently identified
Kazuki Ohno et al.
Biochemical and biophysical research communications, 456(1), 245-249 (2014-12-03)
Hydrogen sulfide (H2S) is a gaseous regulatory factor produced by several enzymes, and plays a pivotal role in processes such as proliferation or vasodilation. Recent reports demonstrated the physiological and pathophysiological functions of H2S in neurons. PTEN is a target
Yuxi Feng et al.
PloS one, 6(2), e16865-e16865 (2011-03-08)
Vasoregression is a hallmark of vascular eye diseases but the mechanisms involved are still largely unknown. We have recently characterized a rat ciliopathy model which develops primary photoreceptor degeneration and secondary vasoregression. To improve the understanding of secondary vasoregression in
Christopher Eginton et al.
Biochemistry, 52(38), 6595-6600 (2013-08-30)
Solvent reorganization can contribute significantly to the energetics of protein-protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a
Ruiguan Wang et al.
Journal of clinical laboratory analysis, 35(6), e23800-e23800 (2021-05-08)
Telomerase is a ribonucleoprotein enzyme responsible for maintenance of telomere length which expressed in more than 85% of cancer cells but undetectable in most normal tissue cells. Therefore, telomerase serves as a diagnostic marker of cancers. Two commonly used telomerase
Chawin Ounkomol et al.
Biophysical journal, 96(3), 1218-1231 (2009-02-03)
We present a multipurpose nanomechanical force probe that combines a sideways-mounted elastic cantilever and an optical-lever detection module with automated micropipette manipulation. It allows us to apply and measure compression, stretching, adhesion, and dissociation forces in the horizontal direction while
Sari Pihlasalo et al.
Analytical biochemistry, 440(1), 6-8 (2013-05-25)
A stable liposome-based time-resolved luminescence resonance energy transfer (TR-LRET) assay was developed based on the interaction of biotinylated lipids and streptavidin. Eu(3+) ion chelated to 4,4,4-trifluoro-1-(2-naphthalenyl)-1,3-butanedione and trioctylphosphine oxide was incorporated into liposomes. Acceptor-labeled streptavidin bound to biotinylated lipids of
Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits
Gobert R P, et al.
Molecular and cellular biology, 29(6), 1538-1553 (2009)
Ria M Cooke et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(13), 5626-5637 (2013-03-29)
Nitric oxide (NO) is an important signaling molecule with a variety of functions in the CNS, including a potential role in modulating neuronal growth and synapse formation. In the present study, we used tractable, identified neurons in the CNS of
Pascal Loyer et al.
International journal of pharmaceutics, 454(2), 727-737 (2013-06-12)
Amphiphilic derivatives of poly(benzyl malate) were synthesized and characterized with the aim of being used as degradable and biocompatible building blocks for the design of functional nanoparticles (NPs). An anti-cancer model drug, doxorubicin, has been successfully encapsulated into the prepared
T I El-Hajj et al.
Neuropediatrics, 39(5), 268-271 (2009-03-19)
Biotin-responsive basal ganglia disease is a rare entity of which 10 cases have been reported in the literature. We report a case of biotin-responsive basal ganglia disease with similarities and differences compared to the previously reported cases by Ozand et
Timothy Sanchez et al.
Methods in enzymology, 524, 205-224 (2013-03-19)
From motility of simple protists to determining the handedness of complex vertebrates, highly conserved eukaryotic cilia and flagella are essential for the reproduction and survival of many biological organisms. Despite extensive studies, the exact mechanism by which individual components coordinate
B Chen et al.
Journal of dairy science, 94(7), 3537-3546 (2011-06-28)
A meta-analysis of the effect of biotin on production outcomes of dairy cattle was conducted following a literature review. A total of 11 studies from 9 papers, with information on the milk production and composition data from a total number
Jun-ichi Maruyama et al.
Plant signaling & behavior, 7(12), 1589-1593 (2012-10-18)
In plants, peroxisomes are the organelles involved in various metabolic processes and physiological functions including β-oxidation, mobilization of seed storage lipids, photorespiration, and hormone biosynthesis. We have recently shown that, in fungi and plants, peroxisomes play a vital role in
Grover L Waldrop et al.
Protein science : a publication of the Protein Society, 21(11), 1597-1619 (2012-09-13)
Biotin is the major cofactor involved in carbon dioxide metabolism. Indeed, biotin-dependent enzymes are ubiquitous in nature and are involved in a myriad of metabolic processes including fatty acid synthesis and gluconeogenesis. The cofactor, itself, is composed of a ureido
Nan Zhao et al.
Nature structural & molecular biology, 25(9), 885-893 (2018-09-05)
Viral infection perturbs host cells and can be used to uncover regulatory mechanisms controlling cellular responses and susceptibility to infections. Using cell biological, biochemical, and genetic tools, we reveal that influenza A virus (IAV) infection induces global transcriptional defects at
Christina M Van Itallie et al.
The Journal of biological chemistry, 288(19), 13775-13788 (2013-04-05)
Biotin ligase tagging with ZO-1 was applied to identify a more complete tight junction proteome. Identical but also different proteins and functional networks were identified near the N and C ends of ZO-1. The ends of ZO-1 are embedded in
Wei Ren et al.
Biosensors & bioelectronics, 49, 380-386 (2013-06-29)
A versatile flow cytometric bead assay (CBA) is developed for sensitive DNA detection by integrating the advantages of hybridization chain reaction (HCR) for enzyme-free signal amplification, flow cytometry for robust and rapid signal readout as well as magnetic beads (MBs)
Liang Tong
Cellular and molecular life sciences : CMLS, 70(5), 863-891 (2012-08-08)
Biotin-dependent carboxylases include acetyl-CoA carboxylase (ACC), propionyl-CoA carboxylase (PCC), 3-methylcrotonyl-CoA carboxylase (MCC), geranyl-CoA carboxylase, pyruvate carboxylase (PC), and urea carboxylase (UC). They contain biotin carboxylase (BC), carboxyltransferase (CT), and biotin-carboxyl carrier protein components. These enzymes are widely distributed in nature
Determination of biotin levels in cerebrospinal fluid samples.
E Livaniou et al.
Journal of pharmaceutical and biomedical analysis, 21(4), 875-879 (2000-03-04)
Konstantina Papadia et al.
Journal of biomedical nanotechnology, 10(5), 871-876 (2014-04-17)
The biotin-Streptavidin (STREP) technique for attachment of monoclonal antibodies (mAbs) (or other ligand types) on liposome surface offers high attachment yield, however it is time consuming and expensive due to the number of steps used and the consumption of large
Wei Xu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(13), 5867-5877 (2013-03-29)
Although the contribution of postsynaptic mechanisms to long-term synaptic plasticity has been studied extensively, understanding the contribution of presynaptic modifications to this process lags behind, primarily because of a lack of techniques with which to directly and quantifiably measure neurotransmitter
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