Search Within
Applied Filters:
Showing 1-30 of 466 results for "


" within Papers
S Martín et al.
International journal for parasitology, 45(13), 831-840 (2015-08-05)
The limitations associated with the use of anthelmintic drugs in the control of gastrotintestinal nematodosis, such as the emergence of anthelmintic resistance, have stimulated the study of the immunological control of many parasites. In the case of Haemonchus contortus, several...
Peng Ye et al.
Molecular and cellular biology, 34(18), 3421-3434 (2014-07-09)
The ubiquitin-proteasome pathway degrades ubiquitinated proteins to remove damaged or misfolded protein and thus plays an important role in the maintenance of many important cellular processes. Because the pathway is also crucial for tumor cell growth and survival, proteasome inhibition...
Annelies Geirnaert et al.
FEMS microbiology ecology, 91(6) (2015-05-23)
Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon microbiota...
Toshinori Hayashi et al.
The International journal of developmental biology, 63(6-7), 281-286 (2019-06-30)
Urodele amphibian newts have an outstanding history as experimental animals in various research fields such as developmental biology and regeneration biology. We have reported a model experimental system using the Spanish newt, Pleurodeles waltl, and it enables reverse/molecular genetics through...
Yoshiaki Nishiya et al.
Bioscience, biotechnology, and biochemistry, 69(11), 2244-2246 (2005-11-25)
We have developed a new enzymatic assay for determining L-cysteine concentration. The method involves the use of betaC-S lyase from Streptococcus anginosus, which catalyzes the alpha,beta-elimination of L-cysteine to hydrogen sulfide, pyruvate, and ammonia. The production of pyruvate is measured...
John M Pryor et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(33), E4537-E4545 (2015-08-05)
Nonhomologous end joining (NHEJ) repairs chromosome breaks and must remain effective in the face of extensive diversity in broken end structures. We show here that this flexibility is often reliant on the ability to direct DNA synthesis across strand breaks...
Oluwasesan Adegoke et al.
Talanta, 146, 780-788 (2015-12-24)
Environmental pollutants, such as the polycyclic aromatic hydrocarbons (PAHs), become widely distributed in the environment after emission from a range of sources, and they have potential biological effects, including toxicity and carcinogenity. In this work, we have demonstrated the analytical...
Masaru Wada et al.
Applied microbiology and biotechnology, 73(1), 48-54 (2006-10-06)
L-Cysteine is an important amino acid both biologically and commercially. Although most amino acids are commercially produced by fermentation, cysteine is mainly produced by protein hydrolysis. However, synthetic or biotechnological products have been preferred in the market. Biotechnological processes for...
Larissa Staurengo-Ferrari et al.
Pharmacological reports : PR, 66(4), 691-698 (2014-06-21)
Several lines of evidence have indicated that nitric oxide (NO) plays complex and diverse roles in modulation of pain/analgesia. However, the roles of charged and uncharged congeners of NO are less well understood. In the present study, the antinociceptive effect...
Emmanuelle Boll et al.
Organic letters, 17(1), 130-133 (2014-12-17)
The use of the N-acetoacetyl protecting group for N-terminal cysteine residue enabled creation of an efficient and mild one-pot native chemical ligation/SEA ligation sequence giving access to large cyclic peptides.
Jennifer Bridwell-Rabb et al.
Biochemistry, 53(30), 4904-4913 (2014-06-28)
Iron-sulfur clusters are ubiquitous protein cofactors with critical cellular functions. The mitochondrial Fe-S assembly complex, which consists of the cysteine desulfurase NFS1 and its accessory protein (ISD11), the Fe-S assembly protein (ISCU2), and frataxin (FXN), converts substrates l-cysteine, ferrous iron...
Sujoy Dutta et al.
PloS one, 9(5), e97580-e97580 (2014-05-17)
Exosomes are nanovesicles originating from multivesicular bodies and are released by all cell types. They contain proteins, lipids, microRNAs, mRNAs and DNA fragments, which act as mediators of intercellular communications by inducing phenotypic changes in recipient cells. Tumor-derived exosomes have...
Nour Mammari et al.
PloS one, 9(6), e98491-e98491 (2014-06-03)
The severity of toxoplasmic infection depends mainly on the immune status of the host, but also on the Toxoplasma gondii strains, which differ by their virulence profile. The relationship between the human host and T. gondii has not yet been...
Thomas F Palmberger et al.
International journal of pharmaceutics, 491(1-2), 318-322 (2015-06-23)
Recently, the cationic polymer thiolated chitosan has been reported to modulate drug absorption by inhibition of intestinal efflux pumps. The objective of this study was to evaluate in vitro and in vivo whether thiolated anionic biopolymers also show an efflux...
Helena Lucena-Padrós et al.
Food microbiology, 53(Pt B), 53-62 (2015-12-19)
The presence and enumeration of halophilic and alkaliphilic bacteria in Spanish-style table-olive fermentations was studied. Twenty 10-tonne fermenters at two large manufacturing companies in Spain, previously studied through both culture dependent and independent (PCR-DGGE) methodologies, were selected. Virtually all this...
Thomas Nauser et al.
Biochemistry, 53(30), 5017-5022 (2014-07-08)
Thioredoxin reductases, important biological redox mediators for two-electron transfers, contain either 2 cysteines or a cysteine (Cys) and a selenocysteine (Sec) at the active site. The incorporation of Sec is metabolically costly, and therefore surprising. We provide here a rationale:...
Daniel L Turman et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(18), 5697-5701 (2015-04-23)
The Fluc family is a set of small membrane proteins forming F(-)-specific electrodiffusive ion channels that rescue microorganisms from F(-) toxicity during exposure to weakly acidic environments. The functional channel is built as a dual-topology homodimer with twofold symmetry parallel...
Charles Ansong et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10153-10158 (2013-05-31)
Characterization of the mature protein complement in cells is crucial for a better understanding of cellular processes on a systems-wide scale. Toward this end, we used single-dimension ultra-high-pressure liquid chromatography mass spectrometry to investigate the comprehensive "intact" proteome of the...
S Peter et al.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 66(3), 449-462 (2015-06-19)
After parturition, uterine bacterial infections lead to inflammatory processes such as subclinical/clinical endometritis with high prevalence in dairy cows. Endometrial epithelial cells participate in this immune response with the production of pro-inflammatory factors. The objective of the present study was...
Anouk A J Hamers et al.
PloS one, 10(8), e0133598-e0133598 (2015-08-05)
Nuclear receptor Nur77, also referred to as NR4A1 or TR3, plays an important role in innate and adaptive immunity. Nur77 is crucial in regulating the T helper 1/regulatory T-cell balance, is expressed in macrophages and drives M2 macrophage polarization. In...
Joseph Verdi et al.
Cell host & microbe, 28(1), 79-88 (2020-05-18)
Trypanosomiasis is a devastating neglected tropical disease affecting livestock and humans. Humans are susceptible to two Trypanosoma brucei subspecies but protected from other trypanosomes by circulating high-density lipoprotein (HDL) complexes called trypanosome lytic factors (TLFs) 1 and 2. TLFs contain...
Joanna Czarnecka et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 822(1-2), 85-90 (2005-07-05)
New method of qualitative and quantitative analysis of nucleotides in human cerebrospinal fluid (CSF), based on the combination of extraction of purines and pyrimidines to the solid phase (SPE) and high-performance liquid chromatography (HPLC), was proposed. Use of SPE and...
Nicole Willems et al.
Arthritis research & therapy, 17, 214-214 (2015-08-21)
Chronic low back pain due to intervertebral disc (IVD) degeneration is associated with increased levels of inflammatory mediators. Current medical treatment consists of oral anti-inflammatory drugs to alleviate pain. In this study, the efficacy and safety of a novel thermoreversible...
Voin Petrović et al.
Journal of inorganic biochemistry, 140, 228-235 (2014-09-01)
Na(+)/K(+)-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na(+) ions out of the cell in exchange for K(+) ions. We explored whether three representative cytotoxic gold(III)...
Valery Kh Akparov et al.
The FEBS journal, 282(7), 1214-1224 (2015-01-27)
The crystal structures of carboxypeptidase T (CpT) complexes with phenylalanine and arginine substrate analogs - benzylsuccinic acid and (2-guanidinoethylmercapto)succinic acid - were determined by the molecular replacement method at resolutions of 1.57 Å and 1.62 Å to clarify the broad...
Zrinka Karačić et al.
Biological chemistry, 393(12), 1523-1532 (2013-05-15)
Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49, involved in protein metabolism and oxidative stress response. DPPIII crystal structure shows the two lobe-like domains separated by a wide cleft. The human enzyme has a...
Alexandra Colin et al.
Current biology : CB, 28(16), 2647-2656 (2018-08-14)
Coordination between actin filaments and microtubules is critical to complete important steps during cell division. For instance, cytoplasmic actin filament dynamics play an active role in the off-center positioning of the spindle during metaphase I in mouse oocytes [1-3] or...
Nir London et al.
Nature chemical biology, 10(12), 1066-1072 (2014-10-27)
Chemical probes that form a covalent bond with a protein target often show enhanced selectivity, potency and utility for biological studies. Despite these advantages, protein-reactive compounds are usually avoided in high-throughput screening campaigns. Here we describe a general method (DOCKovalent)...
Nicholas Kwiatkowski et al.
Nature, 511(7511), 616-620 (2014-07-22)
Tumour oncogenes include transcription factors that co-opt the general transcriptional machinery to sustain the oncogenic state, but direct pharmacological inhibition of transcription factors has so far proven difficult. However, the transcriptional machinery contains various enzymatic cofactors that can be targeted...
S Sofia M Rodrigues et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 16(9), 1880-1888 (2015-04-25)
Immobilization of quantum dots (QDs) onto solid supports could improve their applicability in the development of sensing platforms and solid-phase reactors by allowing the implementation of reusable surfaces and the execution of repetitive procedures. As the reactivity of QDs relies...
Page 1 of 16
Page 1 of 16