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Yan Qiu et al.
STAR protocols, 2(1), 100307-100307 (2021-02-09)
We present a detailed protocol for gene editing in adipocytes using the CRISPR-Cas technology. This protocol describes sgRNA design, preparation of lentiCRISPR-sgRNA vectors, functional validation of sgRNAs, preparation of lentiviruses, and lentiviruses transduction in adipocytes. Moreover, an optimized method of
F Tecles et al.
Research in veterinary science, 100, 12-17 (2015-03-31)
The purpose of this study was to elucidate the possible presence of oxidative stress in cats naturally affected by feline infectious peritonitis (FIP) by investigating two antioxidant biomarkers in serum: paraoxonase-1 (PON1) and total antioxidant capacity (TAC). PON1 was measured
Avinash A Kadam et al.
Bioresource technology, 132, 276-284 (2013-02-16)
Pretreatments to sugarcane bagasse (SCB) such as CaCl2, alkali, ammonia, steam and milling showed 91%, 46%, 47%, 42% and 56% adsorption of Solvent Red 5B (SR5B); 92%, 57%, 58%, 56% and 68% adsorption of simulated dyes mixture (SDM), and 86%
Van T Luu et al.
Bio-protocol, 10(17), e3739-e3739 (2021-03-05)
Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of
Bhavna V Mohite et al.
Applied biochemistry and biotechnology, 169(5), 1497-1511 (2013-01-16)
Bacterial cellulose (BC), a biopolymer, due to its unique properties is valuable for production of vital products in food, textile, medicine, and agriculture. In the present study, the optimal fermentation conditions for enhanced BC production by Gluconacetobacter hansenii NCIM 2529
Stefan Bauernfried et al.
Science (New York, N.Y.), 371(6528) (2020-11-28)
Inflammasomes function as intracellular sensors of pathogen infection or cellular perturbation and thereby play a central role in numerous diseases. Given the high abundance of NLRP1 in epithelial barrier tissues, we screened a diverse panel of viruses for inflammasome activation
Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Shuai Jin et al.
Molecular cell, 79(5), 728-740 (2020-07-30)
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to
Martina Bajerová et al.
Acta poloniae pharmaceutica, 70(2), 271-281 (2013-04-26)
Particles preparation from biodegradable polymers as carriers for the controlled release of drugs has been the focus of many investigations and the subject of a growing field of research in recent years. The aim of this study was to develop
[Ammonium chloride and the calcium chloride loading test for the diagnosis of renal tubular acidosis].
S Takahashi et al.
Nihon rinsho. Japanese journal of clinical medicine, 43(9), 1836-1842 (1985-09-01)
Xiling Yin et al.
STAR protocols, 2(2), 100405-100405 (2021-04-16)
Calcium regulation is a critical process in neurons, and Ca2+ signaling is a major contributor to neurological disorders including Parkinson's disease (PD). Here, combining calcium imaging with whole-cell Ca2+ current recording, we provide a detailed protocol for measuring Ca2+ homeostasis
Grace C Lin et al.
Tissue barriers, 8(2), 1748459-1748459 (2020-04-22)
During the last years, the popularity of saliva has been increasing for its applicability as a diagnostic fluid. Blood biomarker molecules have to cross the blood-saliva barrier (BSB) in order to appear in saliva. The BSB consists of all oral
Diana Baigts Allende et al.
Journal of agricultural and food chemistry, 63(31), 7058-7065 (2015-07-15)
To better tailor gelatins for textural characteristics in (food) gels, their interactions are destabilized by introduction of electrostatic repulsions and creation of affinity sites for calcium to "lock" intermolecular interactions. For that purpose gelatins with various degrees of succinylation are
Pelin Onsekizoglu Bagci et al.
Journal of food science, 80(9), N2071-N2078 (2015-08-05)
Gold nanoparticles (AuNPs) were synthesized at room temperature following a simple, rapid, and green route using fresh-squeezed apple juice as a reducing reagent. The optimal AuNPs, based on the particle color, stability, and color change suitable for colorimetric detection of
Emeline Tanguy et al.
STAR protocols, 2(2), 100464-100464 (2021-04-30)
The glycerophospholipid phosphatidic acid (PA) is a key player in regulated exocytosis, but little is known about its localization at the plasma membrane. Here, we provide a protocol for precisely determining the spatial distribution of PA at exocytotic sites by
Evelien Wynendaele et al.
PloS one, 10(11), e0142071-e0142071 (2015-11-05)
Bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. One group of quorum sensing molecules includes the oligopeptides, which are mainly produced by Gram-positive bacteria. Recently, these quorum sensing peptides were found to
Joseph T Morott et al.
Journal of pharmaceutical sciences, 104(1), 124-134 (2014-11-21)
The primary aim of this research was to produce successfully taste masked formulations of Sildenafil Citrate (SC) using hot-melt extrusion (HME) technology. Multiple screw configurations and polymeric carriers were evaluated for their effects on taste masking efficiency, which was assessed
Koji Nobe et al.
The Journal of pharmacology and experimental therapeutics, 345(1), 62-68 (2013-02-01)
Adiponectin is an adipose tissue-secreted protein and is a multifunctional adipocytokine. However, the association of adiponectin with bladder contraction has not been investigated. In this study, the adiponectin-sense transgenic mouse (Adip-Sen mouse; age, 16-24 weeks; male) and age-matched controls (C57Bl
Siti Shahara Zulfakar et al.
Food microbiology, 34(2), 369-375 (2013-04-02)
Microbial contamination of carcass surfaces occurs during slaughter and post-slaughter processing steps, therefore interventions are needed to enhance meat safety and quality. Although many studies have been done at the macro-level, little is known about specific processes that influence bacterial
Christian Elowsky et al.
Bio-protocol, 7(5), e2150-e2150 (2017-03-05)
Investigation of protein targeting to plastids in plants by confocal laser scanning microscopy (CLSM) can be complicated by numerous sources of artifact, ranging from misinterpretations from in vivo protein over-expression, false fluorescence in cells under stress, and organellar mis-identification. Our
Chao-Han Lai et al.
Annals of surgery, 258(6), 1103-1110 (2013-01-09)
To investigate whether recombinant thrombomodulin containing all the extracellular domains (rTMD123) has therapeutic potential against aneurysm development. The pathogenesis of abdominal aortic aneurysm (AAA) is characterized by chronic inflammation and proteolytic degradation of extracellular matrix. Thrombomodulin, a transmembrane glycoprotein, exerts
Dhanya Jaikumar et al.
International journal of biological macromolecules, 74, 318-326 (2014-12-30)
Injectable, biodegradable scaffolds are required for soft tissue reconstruction owing to its minimally invasive approach. Such a scaffold can mimic the native extracellular matrix (ECM), provide uniform distribution of cells and overcome limitations like donor site morbidity, volume loss, etc.
Satyabrata Das et al.
Nature biotechnology, 38(3), 297-302 (2020-02-26)
The scarcity of donor organs may be addressed in the future by using pigs to grow humanized organs with lower potential for immunological rejection after transplantation in humans. Previous studies have demonstrated that interspecies complementation of rodent blastocysts lacking a
Meral Tunc-Ozdemir et al.
Plant physiology, 161(2), 1010-1020 (2013-02-02)
Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as
Dariusz Lachowski et al.
Scientific reports, 9(1), 7299-7299 (2019-05-16)
Liver fibrosis is characterised by a dense and highly cross-linked extracellular matrix (ECM) which promotes progression of diseases such as hepatocellular carcinoma. The fibrotic microenvironment is characterised by an increased stiffness, with rigidity associated with disease progression. External stiffness is
Zhuqing Wang et al.
Mobile DNA, 10, 17-17 (2019-05-11)
Transposable elements (TEs) make up > 50% of the human genome, and the majority of retrotransposon insertions are truncated and many are located in introns. However, the effects of retrotransposition on the host genes remain incompletely known. We report here that
Yohan Yoon et al.
Meat science, 95(2), 317-322 (2013-06-12)
This study evaluated chemical tenderizers and cooking methods to inactivate Escherichia coli O157:H7 in ground beef patties (model system for non-intact beef). Ground beef was inoculated with E. coli O157:H7 and mixed with (i) nothing (control), (ii) calcium chloride (CC)
Angel Contreras-García et al.
Colloids and surfaces. B, Biointerfaces, 175, 596-605 (2018-12-24)
Biomaterials are frequently evaluated for pro-coagulant activity but usually in the presence of microparticles (MPs), cell-derived vesicles in blood plasma whose phospholipid surfaces allow coagulation factors to set up as functional assemblies. We tested the hypothesis that synthetic anionic surfaces
Carola Städele et al.
Bio-protocol, 7(5), e2151-e2151 (2017-03-05)
This is a detailed protocol explaining how to perform extracellular axon stimulations as described in Städele and Stein, 2016. The ability to stimulate and record action potentials is essential to electrophysiological examinations of neuronal function. Extracellular stimulation of axons traveling
Patcharin Tep-Areenan et al.
Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 95 Suppl 12, S63-S68 (2013-03-22)
To investigate the effects of gamma-mangostin on vascular tone and its mechanisms in the isolated rat aorta. Aortic rings from male Wistar rats were precontracted with methoxamine. Changes in tension were measured using an isometric force transducer and recorded on
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