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d4184

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Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model.

Maciej J Smialek et al.

Cells, 9(4) (2020-04-23)

Mammalian Pumilio (PUM) proteins are sequence-specific, RNA-binding proteins (RBPs) with wide-ranging roles. They are involved in germ cell development, which has functional implications in development and fertility. Although human PUM1 and PUM2 are closely related to each other and recognize

Expression of activation-induced cytidine deaminase is regulated by cell division, providing a mechanistic basis for division-linked class switch recombination.

James S Rush et al.

Proceedings of the National Academy of Sciences of the United States of America, 102(37), 13242-13247 (2005-09-06)

Class switch recombination (CSR) is the process by which B cells alter the effector function properties of their Ig molecules. The decision to switch to a particular Ig isotype is determined primarily by the mode of B cell activation and

gammaCOP is required for apical protein secretion and epithelial morphogenesis in Drosophila melanogaster.

Nicole C Grieder et al.

PloS one, 3(9), e3241-e3241 (2008-09-20)

There is increasing evidence that tissue-specific modifications of basic cellular functions play an important role in development and disease. To identify the functions of COPI coatomer-mediated membrane trafficking in Drosophila development, we were aiming to create loss-of-function mutations in the

Comparison of eight methods for the extraction of Bacillus atrophaeus spore DNA from eleven common interferents and a common swab.

Helen L Rose et al.

PloS one, 6(7), e22668-e22668 (2011-08-06)

Eight DNA extraction products or methods (Applied Biosystems PrepFiler Forensic DNA Extraction Kit; Bio-Rad Instagene Only, Bio-Rad Instagene & Spin Column Purification; EpiCentre MasterPure DNA & RNA Kit; FujiFilm QuickGene Mini80; Idaho Technologies 1-2-3 Q-Flow Kit; MoBio UltraClean Microbial DNA

Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence.

I A Afonina et al.

BioTechniques, 32(4), 940-944 (2002-04-19)

Here we describe the properties of a novel class of oligonucleotide probes capable of sensitive hybridization-triggered fluorescence. These fluorogenic probes, known commercially as MGB Eclipse probes, are characterized by having a conjugated minor groove binder (MGB) ligand at the 5'-end

Cooperative effects of Th2 cytokines and allergen on normal and asthmatic bronchial epithelial cells.

James L Lordan et al.

Journal of immunology (Baltimore, Md. : 1950), 169(1), 407-414 (2002-06-22)

In sensitized individuals, exposure to allergens such as Dermatophagoides pteronyssinus (Der p) causes Th2 polarization and release of cytokines, including IL-4 and IL-13. Because Der p extracts also have direct effects on epithelial cells, we hypothesized that allergen augments the

Helicobacter Pylori's plasticity zones are novel transposable elements.

Dangeruta Kersulyte et al.

PloS one, 4(9), e6859-e6859 (2009-09-04)

Genes present in only certain strains of a bacterial species can strongly affect cellular phenotypes and evolutionary potentials. One segment that seemed particularly rich in strain-specific genes was found by comparing the first two sequenced Helicobacter pylori genomes (strains 26695

A new device for rapid isolation by size and characterization of rare circulating tumor cells.

Isabelle Desitter et al.

Anticancer research, 31(2), 427-441 (2011-03-08)

Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. The ScreenCell® devices are single-use and low-cost innovative devices that use a filter

Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.

Julia K Veir et al.

Veterinary therapeutics : research in applied veterinary medicine, 8(4), 229-238 (2008-01-10)

T evaluate the effect of supplementation with Enterococcus faecium strain SF68 (NCIMB10415) on immune function, responses to a multivalent vaccine were investigated in kittens given palatability enhancer with or without E. faecium SF68 daily. E. faecium SF68 was detected in

Restoration of adenosine deaminase-deficient human thymocyte development in vitro by inhibition of deoxynucleoside kinases.

Michelle L Joachims et al.

Journal of immunology (Baltimore, Md. : 1950), 181(11), 8153-8161 (2008-11-20)

Mutations in the gene encoding adenosine deaminase (ADA), a purine salvage enzyme, lead to immunodeficiency in humans. Although ADA deficiency has been analyzed in cell culture and murine models, information is lacking concerning its impact on the development of human

Plant DNA barcodes and species resolution in sedges (Carex, Cyperaceae).

Julian R Starr et al.

Molecular ecology resources, 9 Suppl s1, 151-163 (2009-05-01)

We investigate the species discriminatory power of a subset of the proposed plant barcoding loci (matK, rbcL, rpoC1, rpoB, trnH-psbA) in Carex, a cosmopolitan genus that represents one of the three largest plant genera on earth (c. 2000 species). To

The cholesterol-regulated StarD4 gene encodes a StAR-related lipid transfer protein with two closely related homologues, StarD5 and StarD6.

Raymond E Soccio et al.

Proceedings of the National Academy of Sciences of the United States of America, 99(10), 6943-6948 (2002-05-16)

Using cDNA microarrays, we identified StarD4 as a gene whose expression decreased more than 2-fold in the livers of mice fed a high-cholesterol diet. StarD4 expression in cultured 3T3 cells was also sterol-regulated, and known sterol regulatory element binding protein

Curcumin induces cell death in esophageal cancer cells through modulating Notch signaling.

Dharmalingam Subramaniam et al.

PloS one, 7(2), e30590-e30590 (2012-03-01)

Curcumin inhibits the growth of esophageal cancer cell lines; however, the mechanism of action is not well understood. It is becoming increasingly clear that aberrant activation of Notch signaling has been associated with the development of esophageal cancer. Here, we

Kinesin KIF18A is a novel PUM-regulated target promoting mitotic progression and survival of a human male germ cell line.

Maciej Jerzy Smialek et al.

Journal of cell science, 133(7) (2020-02-26)

Regulation of proliferation, apoptosis and cell cycle is crucial for the physiology of germ cells. Their malfunction contributes to infertility and germ cell tumours. The kinesin KIF18A is an important regulator of those processes in animal germ cells. Post-transcriptional regulation

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