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Aurélia Cassany et al.
Journal of virology, 89(3), 1719-1730 (2014-11-21)
In this study, we characterized the molecular basis for binding of adenovirus (AdV) to the cytoplasmic face of the nuclear pore complex (NPC), a key step during delivery of the viral genome into the nucleus. We used RNA interference (RNAi)
Masayuki Saito et al.
Bioscience, biotechnology, and biochemistry, 76(9), 1777-1780 (2012-09-14)
The visualization of nuclear architecture, which changes dynamically depending on the physiological and the pathological situation, remains an important challenge. Here we report that exposure of fluoresceinisothiocyanate-labeled polyethylenimine (FITC-PEI) to digitonin-permeabilized cervical cancer HeLa cells enable rapid detection of the
Florian Herrmann et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 18(13), 1191-1196 (2011-10-05)
In phytomedicine complex extracts consisting of phenolics, monoterpenes or saponins are traditionally used. It is often impossible to attribute the biological activity of an extract to one or few compounds. As an explanation of the superior activity of extracts, a
Ramona Madalina Babes et al.
Cell cycle (Georgetown, Tex.), 17(6), 766-779 (2018-02-09)
Human leukemia Jurkat T cells were analyzed for apoptosis and cell cycle by flow cytometry, using the Annexin V/propidium iodide (PI) standard assay, and a simple PI staining in Triton X-100/digitonin-enriched PI/RNase buffer, respectively. Cells treated with doxorubicin or menadione
Michael L van de Weijer et al.
Nature communications, 5, 3832-3832 (2014-05-09)
Misfolded ER proteins are retrotranslocated into the cytosol for degradation via the ubiquitin-proteasome system. The human cytomegalovirus protein US11 exploits this ER-associated protein degradation (ERAD) pathway to downregulate HLA class I molecules in virus-infected cells, thereby evading elimination by cytotoxic
Sankar Bhattacharyya et al.
The Journal of general virology, 95(Pt 1), 71-79 (2013-10-12)
Japanese encephalitis virus (JEV) infection-induced encephalitis causes extensive death or long-term neurological damage, especially among children, in south and south-east Asia. Infection of mammalian cells has shown induction of an unfolded protein response (UPR), presumably leading to programmed cell death
Chongshu Jian et al.
Cell metabolism, 31(5), 892-908 (2020-05-07)
Nonalcoholic steatohepatitis (NASH) is becoming one of the leading causes of hepatocellular carcinoma (HCC). Sorafenib is the only first-line therapy for advanced HCC despite its serious adverse effects. Here, we report that at an equivalent of approximately one-tenth the clinical
J Liu et al.
Methods (San Diego, Calif.), 19(3), 403-409 (1999-12-02)
The application of a cell permeabilization technique to the analysis of nuclear import has led to many major breakthroughs in our understanding of this trafficking pathway. Digitonin permeabilization maintains the nucleus in a state competent for faithful, signal-dependent translocation through
Haraldur H Sigurðsson et al.
Molecular cancer research : MCR, 13(1), 63-77 (2014-08-22)
Despite the frequent expression of N-terminally truncated ErbB2 (ΔNErbB2/p95HER2) in breast cancer and its association with Herceptin resistance and poor prognosis, it remains poorly understood how ΔNErbB2 affects chemotherapy-induced cell death. Previously it was shown that ΔNErbB2 upregulates acid extrusion
Chia-Ling Hsieh et al.
Nucleic acids research, 39(10), 4048-4062 (2011-01-27)
The centromere is a highly specialized chromosomal element that is essential for chromosome segregation during mitosis. Centromere integrity must therefore be properly preserved and is strictly dependent upon the establishment and maintenance of surrounding chromatin structure. Here we identify WDHD1
Yu-Ting Chang et al.
World journal of gastroenterology, 21(32), 9534-9543 (2015-09-04)
To investigate how the saturated and unsaturated fatty acid composition influences the susceptibility of developing acute pancreatitis. Primary pancreatic acinar cells were treated with low and high concentrations of different saturated and unsaturated fatty acids, and changes in the cytosolic
Antonio Carlos Ribeiro-Filho et al.
BMC pharmacology & toxicology, 17(1), 29-29 (2016-07-08)
All hematopoietic cells express P2 receptors, however pharmacological characteristics such as expression and affinity in granulocytes are unknown. Pharmacological characteristics of P2 receptors were evaluated by Ca(2+) measurements using Fura-2 fluorophore. P2 receptors expression were analyzed by flow cytometry and
Tetsuji Moriyama et al.
Biochemical and biophysical research communications, 462(3), 201-207 (2015-05-12)
Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its
Sergio A Mojica et al.
Molecular biology of the cell, 26(10), 1918-1934 (2015-03-20)
SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci-infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP-transfected HeLa cells indicate that SINC targets the inner
Noomi Asker et al.
Environmental toxicology and chemistry, 34(7), 1511-1523 (2015-02-11)
A broad biomarker approach was applied to study the effects of marine pollution along the Swedish west coast using the teleost eelpout (Zoarces viviparus) as the sentinel species. Measurements were performed on different biological levels, from the molecular to the
Stephanie Campbell et al.
Journal of virology, 88(15), 8667-8677 (2014-05-23)
Apoptosis is a tightly regulated process that plays a crucial role in the removal of virus-infected cells, a process controlled by both pro- and antiapoptotic members of the Bcl-2 family. The proapoptotic proteins Bak and Bax are regulated by antiapoptotic
Yair Botbol et al.
Autophagy, 11(10), 1864-1877 (2015-09-24)
Macroautophagy is a cellular process that mediates degradation in the lysosome of cytoplasmic components including proteins and organelles. Previous studies have shown that macroautophagy is induced in activated T cells to regulate organelle homeostasis and the cell's energy metabolism. However
Satoshi Kanda et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5886-E5895 (2016-09-22)
Unconventional mRNA splicing on the endoplasmic reticulum (ER) membrane is the sole conserved mechanism in eukaryotes to transmit information regarding misfolded protein accumulation to the nucleus to activate the stress response. In metazoans, the unspliced form of X-box-binding protein 1
Eri Takeda et al.
PloS one, 6(11), e27815-e27815 (2011-11-24)
Vpr, an accessory protein of human immunodeficiency virus type 1, is a multifunctional protein that plays an important role in viral replication. We have previously shown that the region between residues 17 and 74 of Vpr (Vpr(N17C74)) contained a bona
Kazuo Tatebayashi et al.
Nature communications, 6, 6975-6975 (2015-04-22)
The yeast high osmolarity glycerol (HOG) pathway activates the Hog1 MAP kinase, which coordinates adaptation to high osmolarity conditions. Here we demonstrate that the four-transmembrane (TM) domain protein Sho1 is an osmosensor in the HKR1 sub-branch of the HOG pathway.
Elin Rönnberg et al.
International archives of allergy and immunology, 165(1), 68-74 (2014-10-25)
Many of the functions attributed to mast cells depend on the various pro-inflammatory mediators that are secreted upon mast cell activation. These include a panel of mast cell-specific proteases. In addition, recent studies have indicated that murine mast cells also
S Hussain et al.
The Journal of antimicrobial chemotherapy, 70(1), 136-152 (2014-09-17)
Drugs that target host cell processes can be employed to complement drugs that specifically target viruses, and iminosugar compounds that inhibit host α-glucosidases have been reported to show antiviral activity against multiple viruses. Here the effect and mechanism of two
Gregory T Robertson et al.
Infection and immunity, 82(7), 2935-2948 (2014-04-30)
The Francisella FTT0831c/FTL_0325 gene encodes amino acid motifs to suggest it is a lipoprotein and that it may interact with the bacterial cell wall as a member of the OmpA-like protein family. Previous studies have suggested that FTT0831c is surface
K Kluckova et al.
Cell death & disease, 6, e1749-e1749 (2015-05-08)
Respiratory complex II (CII, succinate dehydrogenase, SDH) inhibition can induce cell death, but the mechanistic details need clarification. To elucidate the role of reactive oxygen species (ROS) formation upon the ubiquinone-binding (Qp) site blockade, we substituted CII subunit C (SDHC)
M Yokono et al.
Nature communications, 6, 6675-6675 (2015-03-27)
Throughout the history of oxygen evolution, two types of photosystem reaction centres (PSI and PSII) have worked in a coordinated manner. The oxygen evolving centre is an integral part of PSII, and extracts an electron from water. PSI accepts the
Ana Victoria Lechuga-Vieco et al.
Science advances, 6(31), eaba5345-eaba5345 (2020-08-25)
Heteroplasmy, multiple variants of mitochondrial DNA (mtDNA) in the same cytoplasm, may be naturally generated by mutations but is counteracted by a genetic mtDNA bottleneck during oocyte development. Engineered heteroplasmic mice with nonpathological mtDNA variants reveal a nonrandom tissue-specific mtDNA
Nunnapas Jiwlawat et al.
Biotechnology and bioengineering, 116(9), 2377-2392 (2019-05-28)
Recent advances in bioengineering have enabled cell culture systems that more closely mimic the native cellular environment. Here, we demonstrated that human induced pluripotent stem cell (iPSC)-derived myogenic progenitors formed highly-aligned myotubes and contracted when seeded on two-dimensional micropatterned platforms.
Yael Hacohen et al.
Neurology, 85(11), 967-975 (2015-08-25)
To assess the clinical and immunologic findings in children with voltage-gated potassium channel (VGKC)-complex antibodies (Abs). Thirty-nine of 363 sera, referred from 2 pediatric centers from 2007 to 2013, had been reported positive (.100 pM) for VGKC-complex Abs. Medical records
Ying-Nai Wang et al.
The Journal of biological chemistry, 287(20), 16869-16879 (2012-03-28)
Nuclear localization of multiple receptor-tyrosine kinases (RTKs), such as EGF receptor (EGFR), ErbB-2, FGF receptor (FGFR), and many others, has been reported by several groups. We previously showed that cell surface EGFR is trafficked to the nucleus through a retrograde
Cresten B Mansfeldt et al.
Applied and environmental microbiology, 80(19), 6062-6072 (2014-07-27)
A cDNA-microarray was designed and used to monitor the transcriptomic profile of Dehalococcoides mccartyi strain 195 (in a mixed community) respiring various chlorinated organics, including chloroethenes and 2,3-dichlorophenol. The cultures were continuously fed in order to establish steady-state respiration rates
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