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Ayokunle O Olanrewaju et al.
ACS sensors, 5(4), 952-959 (2020-04-07)
Poor adherence to pre-exposure prophylaxis (PrEP) and antiretroviral therapy (ART) can lead to human immunodeficiency virus (HIV) acquisition and emergence of drug-resistant infections, respectively. Measurement of antiviral drug levels provides objective adherence information that may help prevent adverse health outcomes....
Sandra Reitmeier et al.
STAR protocols, 1(3), 100148-100148 (2020-12-31)
Targeted sequencing of 16S rRNA genes enables the analysis of microbiomes. Here, we describe a protocol for the collection, storage, and preparation of fecal samples. We describe how we cluster similar sequences and assign bacterial taxonomies. Using diversity analysis and...
Ajay V Maker et al.
Journal of the American College of Surgeons, 228(5), 721-729 (2019-02-23)
Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay...
Alison G Greene et al.
Experimental eye research, 201, 108349-108349 (2020-11-15)
Pseudoexfoliation syndrome (PXF) is the most common cause of secondary open angle glaucoma worldwide. Single nucleotide polymorphisms (SNPs) in the gene Lysyl oxidase like 1 (LOXL1) are strongly associated with the development of pseudoexfoliation glaucoma (PXFG). However, these SNPs are...
Fiona McDonnell et al.
PloS one, 11(4), e0153354-e0153354 (2016-04-29)
Fibrosis and a hypoxic environment are associated with the trabecular meshwork (TM) region in the blinding disease glaucoma. Hypoxia has been shown to alter DNA methylation, an epigenetic mechanism involved in regulating gene expression such as the pro-fibrotic transforming growth...
Maeve O'Huallachain et al.
Communications biology, 3(1), 213-213 (2020-05-10)
Single-cell omics provide insight into cellular heterogeneity and function. Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins...
Suzanne Paz et al.
Molecular and cellular biology, 29(12), 3401-3412 (2009-04-22)
Induction of the antiviral interferon response is initiated upon recognition of viral RNA structures by the RIG-I or Mda-5 DEX(D/H) helicases. A complex signaling cascade then converges at the mitochondrial adapter MAVS, culminating in the activation of the IRF and...
Venkataramana Mudili et al.
Journal of the science of food and agriculture, 94(13), 2674-2683 (2014-03-13)
In this study, mould incidence and mycotoxin contamination were determined in freshly harvested maize samples collected from different agroclimatic regions of India. A total of 150 freshly harvested maize samples from major maize-growing areas of India (Karnataka, Andhra Pradesh and...
Ayokunle O Olanrewaju et al.
Virology journal, 18(1), 77-77 (2021-04-17)
Maintaining adequate drug adherence is crucial to ensure the HIV prevention benefits of pre-exposure prophylaxis (PrEP). We developed an enzymatic assay for rapidly measuring tenofovir-diphosphate (TFV-DP) concentrations-a metabolite that indicates long-term PrEP adherence. The study was conducted at the Madison...
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 8-8 (1989)
Blanca R Lopez et al.
Journal of microbiological methods, 135, 96-104 (2017-02-25)
Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of...
The Field Cricket Gryllus assimilis and Two New Sister Species (Orthoptera: Gryllidae)
Weissman, et al.
Annals of the Entomological Society of America, 102, 367-380 (2009)
Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains...
Billions and billions sold: Pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman, et al.
Zootaxa, 3504(1), 67-88 (2012)
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar...
Unal Egeli et al.
Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from...
Gustav Johansson et al.
Clinical chemistry, 66(9), 1228-1237 (2020-08-21)
Immune repertoire sequencing of the T-cell receptor can identify clonotypes that have expanded as a result of antigen recognition or hematological malignancies. However, current sequencing protocols display limitations with nonuniform amplification and polymerase-induced errors during sequencing. Here, we developed a...
Ozgur Vatan et al.
Tumori, 93(5), 473-477 (2007-11-28)
Lung cancer is one of the most common cancers and has became a predominant cause of cancer-related death throughout the world. The k-ras codon 12 mutation, which is the most common lung cancer mutation, is found in 15 to 30%...
Fiona S McDonnell et al.
Journal of glaucoma, 25(10), e834-e842 (2016-10-18)
Glaucoma is an optic neuropathy that affects 60 million people worldwide. There is an underlying fibrosis associated with the lamina cribrosa (LC) in glaucoma. DNA methylation is well established in regulating fibrosis and may be a therapeutic target for glaucoma....