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Cultrex® 96-Well 3D Spheroid BME Cell Invasion Assay Protocol
The 96 Well 3D Spheroid BME Cell Invasion Assay utilizes 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells.
Cultrex® 3-D Spheroid Colorimetric Proliferation/Viability Assay Protocol
The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers high content format for inducing tumor spheroid formation and quantitating cell viability within the spheroids by absorbance using MTT.
TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol
A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available
In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol
3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.
Generating organotypic 3D skin cultures as an in-vitro model of the human epidermis
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
Cultrex® 3-D Culture Cell Harvesting Kit Protocol
Cultrex™ 3-D Culture Cell Harvesting Kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3-D Culture Matrix™ BME or Laminin I for subsequent biochemical analysis.
Laminin Coating Protocol
Follow this laminin coating protocol for culture ware or neurospheres. Get laminin coating product recommendations and concentrations and troubleshoot with our FAQ.
Tumor Spheroid Formation Assay
3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.