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Rat Dermal Fibroblasts (RDF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Cryopreservation of Cell Lines
Cell culture protocol for freezing cell lines at high cell viabilities using cryopreservation reagents such as Sigma DMSO. Cryopreservation is a method whereby cells are frozen, maintaining their viability, until they are defrosted months or years later. Free ECACC handbook...
Coating Tissue Culture Plates with Vitronectin
Vitronectin is used to coat culture vessels for cells attachment.
Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines
Cell culture protocol for passaging and splitting semi-adherent cell lines using trypsin EDTA.
StableCell™ Trypsin Solutions
StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.
A Guide to Successful Cell Culture
Guidelines and best practices, brought to you by ECACC and us.
How to make and use gradients of Percoll
In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Cell Culture Protocol 1: Proper Aseptic Technique and Sterile Handling of Cells
Cell culture protocol for proper aseptic technique to maintain a sterile environment and to prevent cell culture contamination. Free ECACC handbook download.
How to Make and Use Percoll Gradients
How to use percoll to prepare a gradient