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Hybridoma Fusion Partners Cell Lines
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com
L-15 Media Formulation
L-15 Media Formulation
McCoy's 5A Modified Media Formulation
McCoy's 5A Modified Media Formulation
Cell Culture Fundamentals: Sourcing of Cell Lines
The European Collection of Authenticated Cell Cultures (ECACC) is one of the world’s largest biological cell bank sources supplying a diverse range of authenticated cell lines all offered through Sigma Aldrich. Free ECACC handbook download.
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
Selenium in Cell Culture
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
Lipid-induced Insulin Resistance
Lipid Induced Insulin Resistance
NF-κB and Inflammation
Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.
Vitamin B12, Cobalamin, in Cell Culture
Importance and uses of Vitamin B12 in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Fatty Acid Synthesis and Metabolism in Cancer Cells
Information on fatty acid synthesis and metabolism in cancer cells. Learn how proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sources or are...
Cell Culture Protocol 5: Subculture of Suspension Cell Lines
Cell culture protocol for passaging and splitting suspension cell lines
Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Albumin in Cell Culture
This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and...
Cell Types & Culture Characteristics
An overview of how to classify various cell types (immortalized, primary or stem cells) based on cellular morphology characteristics such as epithelial, endothelial, neuronal or fibroblast. Cell growth characteristics also discussed. Free ECACC handbook download.
Fundamental Cell Culture Techniques
An overview of the common best practices for cell culture and common mistakes to avoid in the cell culture laboratory. Fee ECACC handbook download.
Vitamin B6, Pyridoxal and Pyridoxine in Cell Culture
This page segues to comprehensive insights on how Vitamin B6, Pyridoxal and Pyridoxine affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of...
G-418 Solution Protocol
In this Biochemica article, the dose-response curve of different concentrations of G-418 solution were tested for their ability to stop growth of the adherent PC-3 prostate cancer cell line. Please also refer to the package insert.
Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems
Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.
Lipoic (Thioctic) Acid, in Cell Culture
How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it...
Cell Growth to Confluence
Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads...
Citrate Serum-Free Medium Supplement
Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.
Applications of Percoll in Other Cell Types
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Gastrointestinal Organoid Biobank
Browse over 50 patient-derived human intestinal organoids from our PDO biobank. Various ages, sex, tissue origin and diseases available (normal, tumor organoids, ulcerative colitis).
In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Fetal Bovine Serum Basics
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Cell Culture Contamination Troubleshooting
Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.
Cell Culture FAQs: Bacterial Endotoxin Contamination
What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when...
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
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