Mammalian cell culture

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L-15 Media Formulation

McCoy's 5A Modified Media Formulation

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Cell Culture FAQs: Bacterial Endotoxin Contamination

What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Cell Counting Using a Hemocytometer

Cell counting protocol using a hemocytometer and trypan blue for measuring cell viability.

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

Cell Culture Contamination Troubleshooting

Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.

Folic Acid Metabolism in Cancer

This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Cell Growth to Confluence

Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.

Cell Types & Culture Characteristics

An overview of how to classify various cell types (immortalized, primary or stem cells) based on cellular morphology characteristics such as epithelial, endothelial, neuronal or fibroblast. Cell growth characteristics also discussed. Free ECACC handbook download.

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic

Fundamental Cell Culture Techniques

An overview of the common best practices for cell culture and common mistakes to avoid in the cell culture laboratory. Fee ECACC handbook download.

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Insulin-like Growth Factors (IGF)

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and

NF-κB and Inflammation

Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.

Selenium in Cell Culture

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Corning® High Content Screening Microplates – Frequently Asked Questions

A frequently asked questions guide for users of the Corning® high content screening microplates.

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

Mammary Epithelial Cell Growth Medium

Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Inhibition of Cell Wall Biosynthesis by Antibiotics

Fatty Acid Synthesis and Metabolism in Cancer Cells

Information on fatty acid synthesis and metabolism in cancer cells. Learn how proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sources or are

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

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