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Applications:Nucleic acid labeling and detection
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DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
DIG Northern Starter Kit Protocol & Troubleshooting
In general, it is possible to use DNA probes for northern blots (PCR or random-primed DNA probes). It is however important to keep in mind that DNA probes are best detecting abundant messages, such as housekeeping genes.
DIG RNA Labeling Mix Protocol Troubleshooting
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
PTK/PTP Expression Profiling
PTK/PTP Expression Profiling
NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting
Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
Northern and Southern Blot Protocols & Introduction
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
NBT Protocol & Troubleshooting
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.