Primary cell culture

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In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation. Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Lung Fibroblasts (HLF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Epidermal Keratinocytes (HEK) Culture Protocol

Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Mononuclear Cells – Periphery Blood (HMNC-PB) Culture Protocol

Technical information for working with human mononuclear cells – periphery blood including thawing, subculturing and cryopreservation

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol

Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol

Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation

Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Primary Human Hepatocytes Culture Protocol

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

Human Epidermal Melanocytes (HEM) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Human Chondrocytes (HC) Culture Protocol

Cryovial handling precautions prevent injuries from liquid nitrogen bursts.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

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