Protein and nucleic acid interactions

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Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

How to Optimize Duolink® PLA for Flow Cytometry Detection

Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.

Duolink® Proximity Ligation Assay Instructional Video

This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.

Chromatin Immunoprecipitation (ChIP) Troubleshooting Tips

ChIP-X: Cross-linking proteins to DNA and cell lysis

X-ChIP: Cross-linking proteins to DNA and cell lysis

Fragmentation of chromatin for ChIP applications

Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)

ChIC/CUT&RUN Kits

Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.

Chromatin Immunoprecipitation (ChIP) Troubleshooting

Get answers to frequently asked questions about chromatin immunoprecipitation (ChIP), including issues with sonication and DNA shearing.

RNA Immunoprecipitation Chip (RIP) Assay

RNA Immunoprecipitation (RIP) is an essential method for analyzing proteins that interact with and modify the function of mRNAs, small RNAs, viral RNAs, or lncRNAs.

How Proximity Ligation Assays (PLA) Work

Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).

MS-Cleavable Cross-Linkers

Sulfoxide-containing MS-cleavable cross-linkers capture protein-protein interactions in native cellular environments, aiding PPI identification.

Tips for Immunofluorescence Protocols

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

Chromatin Immunoprecipitation (ChIP)

Choosing the right ChIP antibody is crucial for successful experiments, even with high-quality antibodies validated for Western blot.

EGFR-Family Receptor Dimerization Studies

Investigate EGFR and HER2 dimerization in EGFR-family using cell lines and colon tissues as model systems.

FLAG® HA System

The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.

ChIC and CUT&RUN Data Analysis Tool Overview

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.

Duolink® PLA Technology for Neuroscience Applications

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

Guides to Cell Number for ChIP and Endpoint Analysis

Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection

General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.

RNA Immunoprecipitation (RIP) FAQs

RNA Immunoprecipitation (RIP) Frequently Asked Questions

Duolink® PLA Flow Cytometry Kits

Duolink® PLA kit enhances flow cytometry for detecting protein interactions accurately.

Protein-protein Interactions Detection with PLA

Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.