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Showing 1-14 of 14 results
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Yeast Growth Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
Yale Immunofluorescence Protocol
Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.
Cancer Research Techniques
By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® PLA Fluorescence Protocol
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
How to Create PLA® Probes
Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.
Yeast Transformation Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.