Protein expression

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Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

NF-κB and Inflammation

Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.

How to Optimize Duolink® PLA for Flow Cytometry Detection

Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.

Duolink® Proximity Ligation Assay Instructional Video

This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Directed Evolution

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Cloning & Expression Vectors

Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.

Melatonin Receptors

We offer many products related to melatonin receptors for your research needs.

EX-CELL® Glycosylation Adjust (GAL+)

Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

Tag Removal Proteases for Recombinant Protein Purification

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

Glycolysis via the Embden-Meyerhof-Parnas Glycolytic Pathway

Review the 10 steps of glycolysis in the Embden-Meyerhof-Parnas glycolytic pathway. Easily compare reaction stages and buy the enzymes for your life science research.

Simplicon™ in vivo Protein Expression System

Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.

How Proximity Ligation Assays (PLA) Work

Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Protein-based Drug Transport

Protein-based drug transporters are expressed in Sf9 cells. Understanding the specific mechanisms of tumor cell transporters is an essential aspect of chemotherapeutic drug design.

Competent Cell Selection & E. coli Markers Guide

Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.

FLAG® Tag Bacterial and Mammalian Expression Vectors

FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.

Mitogen-activated Protein Kinases (MAPKs)

The mitogen-activated protein kinase (MAPK) family consists of stress-activated (SAPK) and MAPKs. Learn about their network of signal transduction cascades that mediate cellular responses to a diverse range of stimuli.

Nitric Oxide Synthases

We offer many products related to nitric oxide synthases for your research needs.

Phosphoprotein Phosphatases (Serine/Threonine)

We offers many products related to phosphoprotein phosphatases (Serine/Threonine) for your research needs.

P2 Receptors: P2Y G-Protein Family Overview

Surface receptors for extracellular nucleotides are called P2 receptors. Learn more about P2Y G protein-coupled receptors, GPCRs, and ionotropic P2 receptors (P2X ligand-gated ion channels, LGICs).

Prostanoid Receptors

We offer many products related to prostanoid receptors for your research needs.

Opioid Receptors

We offers many products related to opioid receptors for your research needs.

MAPKKKs

The extracellular signal regulated kinase (ERK1 and ERK2) pathways are activated by mitogens and play an important role in controlling cell growth and differentiation.

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