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BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Lysing Enzymes
Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that
Phosphatase Inhibitor Cocktail
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
cOmplete™ Lysis-M Protocol & Troubleshooting
cOmplete™ Lysis-M Inhibitor: Low protein concentration in lysate
Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
Roche Protein Stabilization Reagents
Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.