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Showing 1-12 of 12 results
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
SYBR® Green I Dye Quantitative PCR Protocol
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
Multiplex qPCR Protocol
A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA Immunoprecipitation qPCR (RIP- qPCR) brief protocol
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
ChIP-qPCR and Data Analysis
Chromatin Immunoprecipitation quantitative real-time PCR (ChIP-qPCR) is commonly used in studies that focus on specific genes and potential regulatory regions across differing experimental conditions and data analysis. qPCR enables DNA analysis in real time by analyzing fluorescent signal intensities that...
qPCR Using a Single Detection Probe Protocol
A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are...
KAPA PROBE FAST qPCR Kits FAQs
Frequently asked questions (FAQs) for KAPA PROBE FAST qPCR Kits.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by...
SPUD Assay for Detection of Assay Inhibitors Protocol
he SPUD assay is one option for identification of inhibitors that may be present in RNA or DNA samples. The assay is particularly useful when a large number of samples are to be analyzed or when targets are present at...
KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA SYBR® FAST One-Step qRT-PCR Kits.
Custom Molecular Reference Standards and Controls
When developing an MDx assay, it is vital to use reference standards and controls. Standards are used for developing the assay, whereas controls are used to assess final working assay quality. Our custom long DNA and RNA are platform independent...