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RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details...
How qPCR Works
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
Primer and Probe Design
Video: Primer and Probe Design
Direct and Crude Extract qPCR
Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Molecular Beacons
Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Choosing the Right Custom qPCR Probe
There is a wide selection of possible custom probes that can be used for qPCR, and each has advantages for different applications.
PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis method that we use for DNA oligonucleotide manufacturing.
Kapa Biosystems qPCR Reagents and Kits
Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.
Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
Dual-Labeled DNA Probes: Fluorescent Probes
To achieve accurate and reliable results from your real-time quantitative PCR, choose from the broadest range of sequence–specific, fluorescent probes: LightCycler probes, Dual-labeled fluorogenic probes, Molecular Beacons, Scorpions™ probes. All of our fluorescent probes are deprotected, desalted and purified...
PCR/qPCR/dPCR Assay Design
The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and...
Dual-Labeled Probes
Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.