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cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting
Media Sterilization – Plant Tissue Culture Protocol
Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel.
Enzymatic Assay of Alcohol Oxidase (EC
This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.
Enzymatic Assay of Cholesterol Oxidase
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Procedure for Enzymatic Assay of α-Chymotrypsin (EC
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
Enzymatic Assay of Esterase
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Enzymatic Assay of Aprotinin
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Enzymatic Assay of Lyticase
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
Using Duolink™ in Multiwell Plates
When using the Duolink reagents to stain cells grown in multiwell plates, a few modifications to the Duolink protocol, optimized for staining samples deposited on glass slides, have to be made in order to suit the multiwell format. In particular
General Instructions for Affinity Purification Using HiTrap® Columns
General instructions for affinity purification using HiTrap columns from Cytiva.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
cOmplete™ His-Tag Purification Column Protocol & Troubleshooting
Purification Protocols: cOmplete His-Tag Purification Columns are compatible with automated chromatography systems such as ÄKTAexplorer.
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Assay Procedure for Invertase
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Peptide Quick Tips
Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Performing a Small-Scale Expression Screening of Histidine-Tagged Membrane Proteins from E. coli Lysates
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Sepharose® High Performance: Purification with High Resolution
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
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