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REDExtract-N-Amp Plant PCR Kits Protocol
The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Viral RNA Purification Protocol Using GenElute™ Mammalian Total RNA Purification Kits
Instructions for purifying viral RNA using GenElute™ Mammalian Total RNA Purification Kits.
Deoxyribonuclease I
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Therma™ PCR Additive
PCR additive for the annealing and extension steps that reduce scatter and increase product signals.
sybr green jumpstart taq readymix
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
Long and Accurate PCR Amplification of DNA
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
This protocol is for conjugating NHS-ester modifications to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
Standard Reverse Transcription Protocol (Two-step)
Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
Kapa Biosystems Taq DNA Polymerase and PCR Kits
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Extract-N-Amp™ FFPE Tissue PCR Protocol
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
KiCqStart™ Probe qPCR ReadyMix™
KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.
qPCR Gene Expression Using Probe Detection
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Restriction Enzyme Digest Protocol
Restriction enzyme collection has been optimized for digestion using five unique buffers.
NBT Protocol
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), for instance, FastGreen FCF or Nuclear Fast Red.
Reference Standards for Analyzing Polyphenol Catechins
Reference Standards for Analyzing Polyphenol Catechins
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