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Gustavo de Medeiros et al.
Nature communications, 6, 8881-8881 (2015-11-26)
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light
M Bartnikowski et al.
Acta biomaterialia, 27, 66-76 (2015-09-01)
Photocrosslinkable hydrogels are frequently used in cartilage tissue engineering, with crosslinking systems relying on cytotoxic photoinitiators and ultraviolet (UV) light to form permanent hydrogels. These systems are rarely assessed in terms of optimization of photoinitiator or UV dosage, with non-cytotoxic
Valeria Imperato et al.
Genes, 10(6) (2019-06-20)
Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil
Akhil Patel et al.
Acta biomaterialia, 112, 262-273 (2020-06-05)
Bone loss due to trauma and tumors remains a serious clinical concern. Due to limited availability and disease transmission risk with autografts and allografts, calcium phosphate bone fillers and growth factor-based substitute bone grafts are currently used in the clinic.
Nandita Srivastava et al.
Frontiers in chemistry, 9, 761682-761682 (2021-11-16)
Dyes are one of the most hazardous chemicals causing significant environmental pollution and affecting water quality. Majority of the existing methods for dye removal and degradation involve synthetic membranes and use of hazardous chemicals, further resulting in secondary pollution. The
Berenice Talamantes-Becerra et al.
BMC microbiology, 20(1), 114-114 (2020-05-15)
This study demonstrates the use of reduced-representation genotyping to provide preliminary identifications for thermophilic bacterial isolates. The approach combines restriction enzyme digestion and PCR with next-generation sequencing to provide thousands of short-read sequences from across the bacterial genomes. Isolates were
Yi-Fang Zhu et al.
Frontiers in plant science, 12, 698443-698443 (2021-07-30)
The C2H2-type zinc finger transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) plays a critical role in aluminum (Al) resistance and low phosphate (Pi) response mainly through promoting the expression of the malate transporter-encoding gene ARABIDOPSIS THALIANA ALUMINUM ACTIVATED MALATE
Timothy E L Douglas et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum
Caroline Teyssier et al.
Physiologia plantarum, 141(2), 152-165 (2010-10-26)
An integrated physiological and proteomic approach was used to investigate the effects of high gellan gum concentration in the medium during maturation of somatic embryos (SE) of hybrid larch, by comparing embryos incubated in media with a high gellan gum
Marco A Lopez-Heredia et al.
Journal of tissue engineering and regenerative medicine, 12(8), 1825-1834 (2018-04-28)
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.
Jinliang Guo et al.
The New phytologist, 228(1), 179-193 (2020-05-15)
C2H2-type zinc finger transcription factor sensitive to proton rhizotoxicity 1 (STOP1) plays an essential role in aluminium (Al) resistance in Arabidopsis thaliana by controlling the expression of a set of Al-resistance genes, including the malate transporter-encoding gene A. thaliana aluminium activated malate
Timothy E L Douglas et al.
Journal of biomedical materials research. Part A, 106(3), 822-828 (2017-10-24)
Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles
Gregory J Gillispie et al.
Tissue engineering. Part A, 26(23-24), 1349-1358 (2020-09-16)
Bioink printability persists as a limiting factor toward many bioprinting applications. Printing parameter selection is largely user-dependent, and the effect of cell density on printability has not been thoroughly investigated. Recently, methods have been developed to give greater insight into
Akhil Patel et al.
Biotechnology and bioengineering, 115(10), 2654-2667 (2018-07-17)
Despite significant interest in developing extracellular matrix (ECM)-inspired biomaterials to recreate native cell-instructive microenvironments, the major challenge in the biomaterial field is to recapitulate the complex structural and biophysical features of native ECM. These biophysical features include multiscale hierarchy, electrical
Timothy E L Douglas et al.
Biomedical materials (Bristol, England), 12(2), 025015-025015 (2017-02-23)
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum
Ghulam Raza et al.
Plants (Basel, Switzerland), 9(1) (2019-12-29)
The efficient regeneration of plants from commercial genotypes is a pre-requisite for successful genetic transformation, to apply modern crop improvement techniques such as CRISPR-based genome editing. Plant regeneration through the somatic embryogenesis pathway offers an advantage over the organogenesis approach
João T Oliveira et al.
Tissue engineering. Part A, 16(1), 343-353 (2009-08-26)
Gellan gum is a polysaccharide that we have previously proposed for applications in the cartilage tissue engineering field. In this work, gellan gum hydrogels were tested for their ability to be used as injectable systems using simple processing methods, able
Timothy E L Douglas et al.
Journal of tissue engineering and regenerative medicine, 11(5), 1610-1618 (2015-07-16)
Gellan gum hydrogels functionalized with alkaline phosphatase were enzymatically mineralized with phosphates in mineralization medium containing calcium (Ca) and zinc (Zn) to improve their suitability as biomaterials for bone regeneration. The aims of the study were to endow mineralized hydrogels
Ming Fang Hsu et al.
Scientific reports, 8(1), 731-731 (2018-01-18)
The purpose of this study was to develop a gellan gum-based multifunctional embolic agent. Calibrated spherical gellan gum and nanoparticle-containing gellan gum microspheres were prepared via water-in oil emulsification method. Self-assembled nanoparticles composed of short-chain hyaluronic acid and polyethylenimine as
Zhuoran Jiang et al.
ACS applied materials & interfaces, 11(31), 28289-28295 (2019-07-11)
Poly(tetrafluoroethylene) (PTFE) is a unique polymer with highly desirable properties such as resistance to chemical degradation, biocompatibility, hydrophobicity, antistiction, and low friction coefficient. However, due to its high melt viscosity, it is not possible to three-dimensional (3D)-print PTFE structures using
J T Oliveira et al.
Journal of tissue engineering and regenerative medicine, 3(7), 493-500 (2009-07-15)
Gellan gum is a polysaccharide that has been recently proposed by our group for cartilage tissue-engineering applications. It is commonly used in the food and pharmaceutical industry and has the ability to form stable gels without the use of harsh
Shohei Hayashi et al.
Microbes and environments, 26(2), 120-127 (2011-04-20)
The cyanobacterium Synechococcus leopoliensis CCAP1405/1 does not grow on common solid media made of agar, agarose HT, noble agar, gelrite and gelatin, although it grows in liquid media with the same components. The inoculation of S. leopoliensis CCAP1405/1 at a
Timothy E L Douglas et al.
Journal of tissue engineering and regenerative medicine, 10(11), 938-954 (2014-03-13)
Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with
Yi-Jun Qiao et al.
Plant diversity, 42(5), 323-333 (2020-11-03)
Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study
Shreya Kalia et al.
International journal of biological macromolecules, 137, 475-482 (2019-07-02)
Hydrogels are three dimensional polymeric structures and are of great importance to industrial sector due to their signature physiochemical and mechanical properties. Use of natural polysaccharides as the components of hydrogel composite is of great interest due to their non-toxic
Shrikant A Suvase et al.
Journal of microbiology and biotechnology, 20(7), 1086-1091 (2010-07-30)
This study explored the use of gellan gum as an immobilization matrix for production of cyclosporin A (CyA) by immobilized spores and mycelia of Tolypocladium inflatum MTCC 557. Different carriers, such as gellan gum, sodium alginate, celite beads and silica
Léo Guignard et al.
Science (New York, N.Y.), 369(6500) (2020-07-11)
Marine invertebrate ascidians display embryonic reproducibility: Their early embryonic cell lineages are considered invariant and are conserved between distantly related species, despite rapid genomic divergence. Here, we address the drivers of this reproducibility. We used light-sheet imaging and automated cell
Akhil Patel et al.
Journal of biomedical materials research. Part A, 108(12), 2504-2518 (2020-05-18)
Polysaccharides are explored for various tissue engineering applications due to their inherent cytocompatibility and ability to form bulk hydrogels. However, bulk hydrogels offer poor control over their microarchitecture and multiscale hierarchy, parameters important to recreate extracellular matrix-mimetic microenvironment. Here, we
Regulation of Aluminum Resistance in Arabidopsis Involves the SUMOylation of the Zinc Finger Transcription Factor STOP1.
Fang, et al.
Plant Cell, 32, 3921-3938 (2021)
Ken-Ichi Konagaya et al.
Plant biotechnology (Tokyo, Japan), 37(2), 147-156 (2020-08-22)
Sugi (Cryptomeria japonica D. Don) is the most important afforestation coniferous tree in Japan. Coniferous trees normally have a long juvenile period and require a long cultivation time for breeding. Through a traditional breeding project that began in the 1950s
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