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Broder Rühmann et al.
Journal of chromatography. A, 1350, 44-50 (2014-05-28)
A fast carbohydrate screening platform processible in 96-well format is described. The method is suitable for the determination of various carbohydrates out of complex mixtures as obtained by acidic hydrolysis of carbohydrates polymers. The chromatographic conditions for an efficient separation
T Trdan Lušin et al.
Die Pharmazie, 70(2), 94-96 (2015-05-23)
Bazedoxifene is used for the prevention and treatment of osteoporosis. After peroral application, bazedoxifene is metabolized by UDP-glucuronosyltransferases (UGTs) to bazedoxifene-4'-glucuronide (M4) and bazedoxifene-5-glucuronide (M5). It has already been shown that a relatively common UGT1A1*28 polymorphism can considerably affect raloxifene
Charanpreet Kaur et al.
Molecular biology reports, 42(6), 1139-1148 (2015-01-31)
Salt Overly Sensitive (SOS) pathway comprising SOS1, SOS2 and SOS3 genes has been recognized as the key mechanism controlling ion homeostasis under salinity stress. SOS2 component of this pathway encodes a serine/threonine protein kinase that together with SOS3 activates downstream
Toshihiro Kurahashi et al.
Biochemical and biophysical research communications, 452(1), 136-141 (2014-08-26)
Aldehyde reductase (AKR1A), a member of the aldo-keto reductase superfamily, suppresses diabetic complications via a reduction in metabolic intermediates; it also plays a role in ascorbic acid biosynthesis in mice. Because primates cannot synthesize ascorbic acid, a principle role of
Yingyu Wang et al.
Carbohydrate polymers, 127, 215-221 (2015-05-13)
In this study, one polysaccharide (GFP1), with an average molecular weight of 1.4 × 10(5)Da, was isolated from Ginseng fruits. GFP1 was composed of galactose, glucose, rhamnose, and arabinose in a molar ratio of 6.1:2.0:1.1:3.2, and had a backbone mainly
Nguyen Khoi Nguyen et al.
SpringerPlus, 4, 91-91 (2015-03-13)
Kombucha is sweetened black tea that is fermented by a symbiosis of bacteria and yeast embedded within a cellulose membrane. It is considered a health drink in many countries because it is a rich source of vitamins and may have
Jiang Zhu et al.
Plant & cell physiology, 56(4), 727-736 (2015-01-02)
Plants exhibit reduced root growth when exposed to low temperature; however, how low temperature modulates root growth remains to be understood. Our study demonstrated that low temperature reduces both meristem size and cell number, repressing the division potential of meristematic
J R Alonso-Fernandez et al.
Journal of clinical laboratory analysis, 24(2), 106-112 (2010-03-25)
We describe the history and current implementation of an inexpensive thin layer chromatography (TLC) method, vertical sandwich-type continuous/evaporative TLC with fixed mobile phase volume, that is convenient for detecting and identifying reducing sugars of clinical relevance in the paper-borne blood
Hassan Salem et al.
Current biology : CB, 30(15), 2875-2886 (2020-06-06)
Numerous adaptations are gained in light of a symbiotic lifestyle. Here, we investigated the obligate partnership between tortoise leaf beetles (Chrysomelidae: Cassidinae) and their pectinolytic Stammera symbionts to detail how changes to the bacterium's streamlined metabolic range can shape the
Nan Wang et al.
Carbohydrate polymers, 111, 47-55 (2014-07-20)
A neutral polysaccharide fraction (SMPA) prepared from the roots of Salvia miltiorrhiza by DEAE-cellulose and Sephadex G-100 chromatography was tested for its immune enhancing function in N-methyl-N'-nitro-nitrosoguanidine (MNNG)-induced gastric cancer rats by intragastric administration. SMPA (200mg/kg) treatment not only increased
Bioaccessibility of Hg, Cd and As in cooked black scabbard fish and edible crab
Maulvault A, et al.
Food And Chemical Toxicology, 49(11), 2808-2815 (2011)
Yanmei Zou et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5179-5186 (2015-02-06)
In the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) from Andrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells
Julieta Gradinaru et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 71, 35-45 (2015-02-17)
A rapid method for the simultaneous determination of the in vitro activity of the 10 major human liver UDP-glucuronosyltransferase (UGT) enzymes was developed based on the cocktail approach. Specific substrates were first selected for each UGT: etoposide for UGT1A1, chenodeoxycholic
Lorenz Gerber et al.
The New phytologist, 203(4), 1220-1230 (2014-06-13)
The biosynthesis of wood in aspen (Populus) depends on the metabolism of sucrose, which is the main transported form of carbon from source tissues. The largest fraction of the wood biomass is cellulose, which is synthesized from UDP-glucose. Sucrose synthase
Ling Li et al.
Molecular cell, 80(3), 525-540 (2020-10-18)
Well-balanced and timed metabolism is essential for making a high-quality egg. However, the metabolic framework that supports oocyte development remains poorly understood. Here, we obtained the temporal metabolome profiles of mouse oocytes during in vivo maturation by isolating large number of
Lignocellulosic Biorefineries, 86(1), 125-139 (2013)
Thomas Ho Yuen Tam et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(29), E3959-E3968 (2015-07-08)
Land plants develop filamentous cells-root hairs, rhizoids, and caulonemata-at the interface with the soil. Members of the group XI basic helix-loop-helix (bHLH) transcription factors encoded by LOTUS JAPONICUS ROOTHAIRLESS1-LIKE (LRL) genes positively regulate the development of root hairs in the
Sung Chul Choi et al.
Journal of biomedical materials research. Part A, 103(9), 3072-3080 (2015-02-19)
Modulation of both mechanical properties and biocompatibilities of hyaluronic acid (HA) hydrogels is very importance for their applications in biomaterials. Pure HA solution was converted into a hydrogel by using butanediol diglycidyl ether (BDDE) as a crosslinking agent. Mechanical properties
Z Cardman et al.
Applied and environmental microbiology, 80(12), 3749-3756 (2014-04-15)
In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments. In an Arctic fjord (Smeerenburgfjord) of
Hong-Fei Wang et al.
Antonie van Leeuwenhoek, 107(3), 835-843 (2015-01-09)
A white bacterial strain, designated EGI 650022(T), was isolated from the roots of Salsola affinis C. A. Mey, collected from Urumqi City, Xinjiang, north-western China. The strain was found to be aerobic, Gram-stain positive, oxidase-positive and catalase-positive. Cells were non-motile
Guang Zeng et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(9), 6781-6788 (2015-04-04)
In the present study, we isolated and characterized a homogenous polysaccharide (GIAP1) from the alkaline extract of the roots of Glycyrrhiza inflata. The anti-tumor activity of GIAP1 toward human oral cancer SCC-25 cells and the underlying mechanisms were also examined
Foon Yin Lai et al.
Journal of pharmaceutical and biomedical analysis, 114, 355-375 (2015-06-27)
Phenethylamine-based designer drugs are prevalent within the new psychoactive substance market. Characterisation of their metabolites is important in order to identify suitable biomarkers which can be used for better monitoring their consumption. Careful design of in vitro metabolism experiments using
Alexander N Patananan et al.
Archives of biochemistry and biophysics, 569, 32-44 (2015-02-11)
l-Ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule
Thu V Vuong et al.
Biotechnology for biofuels, 13, 51-51 (2020-03-20)
Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4-O-methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries. Accordingly, an enzymatic pathway was
Qingxia Yuan et al.
Carbohydrate polymers, 128, 52-62 (2015-05-26)
Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature
Jie Zhang et al.
Phytochemistry, 108, 157-170 (2014-12-03)
The MeOH extract of defatted shea (Vitellaria paradoxa; Sapotaceae) kernels was investigated for its constituents, and fifteen oleanane-type triterpene acids and glycosides, two steroid glucosides, two pentane-2,4-diol glucosides, seven phenolic compounds, and three sugars, were isolated. The structures of five
P Doig et al.
Infection and immunity, 55(6), 1517-1522 (1987-06-01)
The alginate produced by Pseudomonas aeruginosa has been reported to play a role in the adhesion of this bacterium to epithelial cell surfaces, although some controversy concerning this role exists. To clarify this controversy, we investigated the ability of alginate
Amanda K Chaplin et al.
The Biochemical journal, 469(3), 433-444 (2015-07-25)
Streptomyces lividans displays a distinct dependence on copper to fully initiate morphological development. Evidence has accumulated to implicate the participation of an extracytoplasmic cuproenzyme in morphogenesis. In the present study, we show that GlxA fulfils all criteria to be that
Junyan Li et al.
PLoS genetics, 10(3), e1004232-e1004232 (2014-03-15)
Plant microRNAs (miRNAs) are critical regulators of gene expression, however little attention has been given to the principles governing miRNA silencing efficacy. Here, we utilize the highly conserved Arabidopsis miR159-MYB33/MYB65 regulatory module to explore these principles. Firstly, we show that
Bjarke Follin et al.
Cytotherapy, 17(8), 1104-1118 (2015-06-03)
Clinical trials have documented beneficial effects of mesenchymal stromal cells from bone marrow and adipose tissue (ASCs) as treatment in patients with ischemic heart disease. However, retention of transplanted cells is poor. One potential way to increase cell retention is
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