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Cátia Carreira et al.
Applied and environmental microbiology, 81(6), 2149-2155 (2015-01-18)
Viral abundances in benthic environments are the highest found in aquatic systems. Photosynthetic microbial mats represent benthic environments with high microbial activity and possibly high viral densities, yet viral abundances have not been examined in such systems. Existing extraction procedures
D R Scott et al.
Alimentary pharmacology & therapeutics, 42(11-12), 1315-1326 (2015-10-02)
The gastric H(+) ,K(+) -ATPase is the preferred target for acid suppression. Until recently, the only drugs that effectively inhibited this ATPase were the proton pump inhibitors (PPIs). PPIs are acid-activated prodrugs that require acid protection. Once acid-activated, PPIs bind
Marina Koulikovska et al.
Tissue engineering. Part A, 21(5-6), 1116-1130 (2014-11-21)
Severe shortage of donor corneas for transplantation, particularly in developing countries, has prompted the advancement of bioengineered tissue alternatives. Bioengineered corneas that can withstand transplantation while maintaining transparency and compatibility with host cells, and that are additionally amenable to standardized
Han Zhou et al.
Molecular medicine reports, 11(6), 4381-4388 (2015-02-06)
Polyethylenimine (PEI) has been demonstrated to be an effective non‑viral synthetic polymer for efficient gene delivery amongst various cell types in vitro and in vivo. In the present study, 25 kDa linear PEI (L‑PEI) was used to transfer plasmid DNA (pDNA), encoding the
Andrew Tsz Hang Choy et al.
PloS one, 10(6), e0131827-e0131827 (2015-06-27)
Tissue engineering offers high hopes for the treatment of intervertebral disc (IVD) degeneration. Whereas scaffolds of the disc nucleus and annulus have been extensively studied, a truly biomimetic and mechanically functional biphasic scaffold using naturally occurring extracellular matrix is yet
Ana K Bedran-Russo et al.
American journal of dentistry, 26(5), 244-248 (2014-02-01)
To assess in non-cavitated carious teeth the mechanical properties of dentin matrix by measuring its reduced modulus of elasticity and the effect of dentin biomodification strategies on three dentin matrix zones: caries-affected, apparently normal dentin below caries-affected zone and sound
Ariene A Leme et al.
Journal of biomechanics, 48(10), 2067-2071 (2015-04-15)
Degradation of the adhesive interface contributes to the failure of resin composite restorations. The hydrophilicity of the dentin matrix during and after bonding procedures may result in an adhesive interface that is more prone to degradation over time. This study
Nicholas B Last et al.
Journal of molecular biology, 427(22), 3607-3612 (2015-10-10)
Anion channels and antiporters of the ClC superfamily have been found to be exclusively dimeric in nature, even though each individual monomer contains the complete transport pathway. Here, we describe the destabilization through mutagenesis of the dimer interface of a
Li-na Sun et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 68(8), 1079-1088 (2014-12-03)
Epithelial-mesenchymal transition (EMT) is recognized to play a key role in diabetic nephropathy (DN). Curcumin, the main active component of turmeric extracted from the roots of the Curcuma longa plant, has been reported for its anti-fibrotic effects in kidney fibrosis. The
Krzysztof Symonowicz et al.
Experimental and therapeutic medicine, 8(4), 1039-1046 (2014-09-05)
The aim of the present study was to investigate the immunohistochemical expression of nuclear ubiquitous casein and cyclin-dependent kinases substrate 1 (NUCKS1) in invasive breast carcinoma of no special type, in association with clinicopathological characteristics, including the tumor grade, frequency
Christopher L Mendias et al.
Journal of shoulder and elbow surgery, 24(1), 111-119 (2014-09-07)
A persistent atrophy of muscle fibers and an accumulation of fat, collectively referred to as fatty degeneration, commonly occur in patients with chronic rotator cuff tears. The etiology of fatty degeneration and function of the residual rotator cuff musculature have
Ananda Shankar Bhattacharjee et al.
Biotechnology and bioengineering, 112(8), 1644-1654 (2015-03-03)
To demonstrate elimination of bacterial biofilm on membranes to represent wastewater treatment as well as biofilm formed by antibiotic-resistant bacterial (ARB) to signify medical application, an antibiotic-resistant bacterium and its lytic bacteriophage were isolated from a full-scale wastewater treatment plant.
Heng Zhu et al.
Biomaterials, 53, 688-698 (2015-04-22)
The poly lactic-co-glycolic acid (PLGA) bio-scaffold is a biodegradable scaffold commonly used for tissue repair. However, implanted PLGA scaffolds usually cause serious inflammatory responses around grafts. To improve PLGA scaffold-based tissue repair, it is important to control the PLGA-mediated inflammatory
Dries Bauters et al.
Biochimica et biophysica acta, 1850(7), 1449-1456 (2015-04-15)
Expansion of adipose tissue is dependent on adipogenesis, angiogenesis and extracellular matrix remodeling. A functional role in these processes was suggested for the gelatinase subfamily of the matrix metalloproteinases. Here, we have evaluated a potential role of gelatinase A (MMP-2)
Yíngyún Caì et al.
Journal of virology, 89(1), 844-856 (2014-10-31)
Simian hemorrhagic fever virus (SHFV) causes a severe and almost uniformly fatal viral hemorrhagic fever in Asian macaques but is thought to be nonpathogenic for humans. To date, the SHFV life cycle is almost completely uncharacterized on the molecular level.
Manoranjan Arakha et al.
Scientific reports, 5, 9578-9578 (2015-04-16)
The work investigates the role of interfacial potential in defining antimicrobial propensity of ZnO nanoparticle (ZnONP) against different Gram positive and Gram negative bacteria. ZnONPs with positive and negative surface potential are tested against different bacteria with varying surface potentials
Céline Labouesse et al.
Biophysical journal, 108(10), 2437-2447 (2015-05-21)
The mechanical interaction between adherent cells and their substrate relies on the formation of adhesion sites and on the stabilization of contractile acto-myosin bundles, or stress fibers. The shape of the cell and the orientation of these fibers can be
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Tae Joon Kwak et al.
Scientific reports, 10(1), 20142-20142 (2020-11-21)
Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis. However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface
Hanieh Shirazi et al.
Beilstein journal of nanotechnology, 6, 1677-1689 (2015-10-02)
The unique properties and applications of iron oxide and Au nanoparticles have motivated researchers to synthesize and optimize a combined nanocomposite containing both. By using various polymers such as chitosan, some of the problems of classic core-shell structures (such as
Alice H Huang et al.
Development (Cambridge, England), 142(14), 2431-2441 (2015-06-13)
The long tendons of the limb extend from muscles that reside in the zeugopod (arm/leg) to their skeletal insertions in the autopod (paw). How these connections are established along the length of the limb remains unknown. Here, we show that
Mohammed A Mansour et al.
Experimental cell research, 332(1), 78-88 (2014-12-20)
Invadopodia are specialized actin-based microdomains of the plasma membrane that combine adhesive properties with matrix degrading activities. Proper functioning of the bone, immune, and vascular systems depend on these organelles, and their relevance in cancer cells is linked to tumor
Yolanda Osuna et al.
Bioprocess and biosystems engineering, 38(8), 1437-1445 (2015-03-12)
Aspergillus niger lipase immobilization by covalent binding on chitosan-coated magnetic nanoparticles (CMNP), obtained by one-step co-precipitation, was studied. Hydroxyl and amino groups of support were activated using glycidol and glutaraldehyde, respectively. Fourier transform infrared spectrometry, high-resolution transmission electron microscopy and
Yishan Fang et al.
Biosensors & bioelectronics, 73, 71-78 (2015-06-06)
This work reports on a facile, novel multianalyte electrochemical immunoassay for simultaneous detection of a-fetoprotein (AFP) and human epidermal growth factor receptor type-2 (HER-2) using metal-containing nanomaterials confined in the ordered mesoporous carbon matrix (OMC-M) as labels. Well-dispersed uniform metallic
Rerngchai Arayanarakool et al.
Lab on a chip, 15(14), 2981-2989 (2015-06-09)
Artificial microvasculature, particularly as part of the blood-brain barrier, has a high benefit for pharmacological drug discovery and uptake regulation. We demonstrate the fabrication of tubular structures with patterns of holes, which are capable of mimicking microvasculatures. By using photolithography
Kyle R Pomraning et al.
PloS one, 10(4), e0123188-e0123188 (2015-04-24)
Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the
Helen L May-Simera et al.
Development (Cambridge, England), 142(3), 555-566 (2015-01-22)
Primary cilia have been implicated in the generation of planar cell polarity (PCP). However, variations in the severity of polarity defects in different cilia mutants, coupled with recent demonstrations of non-cilia-related actions of some cilia genes, make it difficult to
Anna Golunova et al.
Biomacromolecules, 16(4), 1146-1156 (2015-03-03)
The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the
Ludmila Perelygina et al.
Journal of virology, 89(10), 5515-5524 (2015-03-06)
Glycoprotein D (gD) plays an essential role in cell entry of many simplexviruses. B virus (Macacine herpesvirus 1) is closely related to herpes simplex virus 1 (HSV-1) and encodes gD, which shares more than 70% amino acid similarity with HSV-1
Jennifer L Wehmeyer et al.
Tissue engineering. Part C, Methods, 21(7), 649-659 (2014-12-05)
Numerous techniques have been reported for preparing and sterilizing amniotic membrane (AM) for use in clinical applications. However, these preparations either do not produce completely sterile tissue or are detrimental to molecules unique to the tissue matrix, thus compromising beneficial
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