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Gregory N Ruegsegger et al.
JCI insight, 4(18) (2019-09-20)
Insulin resistance associates with increased risk for cognitive decline and dementia; however, the underpinning mechanisms for this increased risk remain to be fully defined. As insulin resistance impairs mitochondrial oxidative metabolism and increases ROS in skeletal muscle, we considered whether
K E Sawin et al.
The Journal of cell biology, 142(2), 457-471 (1998-07-29)
To investigate the role of microtubules in regulating cell polarity in Schizosaccharomyces pombe, we have developed a system in which normally cylindrical fission yeast synchronously form branched cells at high frequency upon treatment with the microtubule-depolymerizing drug thiabendazole (TBZ). Branching
Julieta Saba et al.
Journal of neurochemistry, 146(6), 686-702 (2018-06-01)
Astrocytes are glial cells that help maintain brain homeostasis and become reactive in neurodegenerative processes releasing both harmful and beneficial factors. We have demonstrated that brain-derived neurotrophic factor (BDNF) expression is induced by melanocortins in astrocytes but BDNF actions in
Ting-Ting Du et al.
Autophagy, 11(10), 1803-1820 (2015-09-18)
Loss-of-function mutations in the gene encoding GBA (glucocerebrosidase, β, acid), the enzyme deficient in the lysosomal storage disorder Gaucher disease, elevate the risk of Parkinson disease (PD), which is characterized by the misprocessing of SNCA/α-synuclein. However, the mechanistic link between
Coralie Fouquet et al.
Bio-protocol, 9(8) (2019-05-28)
In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes
Zahra Dargaei et al.
Journal of neurophysiology, 112(11), 2680-2696 (2014-09-05)
In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for the electrically coupled bag cell neurons in the reproductive system of the marine snail, Aplysia californica. These neuroendocrine
Georg E Fantner et al.
Nature nanotechnology, 5(4), 280-285 (2010-03-17)
Observations of real-time changes in living cells have contributed much to the field of cellular biology. The ability to image whole, living cells with nanometre resolution on a timescale that is relevant to dynamic cellular processes has so far been
Haibin Wang et al.
Autophagy, 9(12), 1975-1982 (2013-11-05)
The physiological relationship between autophagy and programmed cell death during C. elegans development is poorly understood. In C. elegans, 131 somatic cells and a large number of germline cells undergo programmed cell death. Autophagy genes function in the removal of
A I Marcus et al.
Molecular biology of the cell, 14(4), 1717-1726 (2003-04-11)
Motor proteins have been implicated in various aspects of mitosis, including spindle assembly and chromosome segregation. Here, we show that acentrosomal Arabidopsis cells that are mutant for the kinesin, ATK1, lack microtubule accumulation at the predicted spindle poles during prophase
Claire Eschbach et al.
eLife, 10 (2021-11-11)
Animal behavior is shaped both by evolution and by individual experience. Parallel brain pathways encode innate and learned valences of cues, but the way in which they are integrated during action-selection is not well understood. We used electron microscopy to
A Kamal et al.
The Journal of cell biology, 142(4), 937-947 (1998-08-29)
Previously we reported that annexin VI is required for the budding of clathrin-coated pits from human fibroblast plasma membranes in vitro. Here we show that annexin VI bound to the NH2-terminal 28-kD portion of membrane spectrin is as effective as
Ruolin Li et al.
Autophagy, 1-17 (2021-06-08)
Olfactory dysfunction, one of the earliest non-motor symptoms of Parkinson disease (PD), is accompanied by abnormal deposition of SNCA/α-synuclein in the olfactory bulb (OB). The macroautophagy/autophagy-lysosome pathway (ALP) plays an important role in degrading pathological SNCA and modulating this pathway
Daniel Moreno-Blas et al.
Aging, 11(16), 6175-6198 (2019-08-31)
Senescent cells accumulate in various tissues and organs with aging altering surrounding tissue due to an active secretome, and at least in mice their elimination extends healthy lifespan and ameliorates several chronic diseases. Whether all cell types senesce, including post-mitotic
C Schmidt et al.
The Journal of biological chemistry, 273(30), 19146-19152 (1998-07-21)
Sialylation of glycoproteins and glycolipids plays an important role during development, regeneration, and pathogenesis of diseases. During times of intense plasticity within the nervous system, such as development and regeneration, sialylation of neural cells is distinct from the time of
Kara Wolfe et al.
Biochemical and biophysical research communications, 516(1), 50-56 (2019-06-15)
Compartmentalization is vital for biological systems at multiple levels, including biochemical reactions in metabolism. Organelle-based compartments such as mitochondria and peroxisomes sequester the responsible enzymes and increase the efficiency of metabolism while simultaneously protecting the cell from dangerous intermediates, such
Narayana Pineda-Ramírez et al.
Molecular neurobiology, 57(2), 1055-1069 (2019-11-02)
During cerebral ischemia, oxygen and glucose levels decrease, producing many consequences such as the generation of reactive oxygen species, tissue injury, and the general metabolism collapse. Resveratrol triggers signaling dependent on the protein kinase activated by adenosine monophosphate (AMPK), the
In vivo tracking of mesenchymal stem cells labeled with a novel chitosan-coated superparamagnetic iron oxide nanoparticles using 3.0 T MRI
Reddy AM, et al.
Journal of Korean Medical Science, 25(2), 211-219 (2010)
Nataša Savić et al.
Bio-protocol, 9(1) (2019-01-25)
The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single guide (sg)RNA, and introduces a site-specific double-stranded break (DSB). In mammalian cells, DSBs
Michael Gal et al.
Clinical medicine insights. Reproductive health, 8, 37-44 (2014-07-06)
Ketoconazole (KCZ) is a known inhibitor of steroidogenic P450 enzymes in the adrenal cortex and the gonads. Previous studies examined the potential clinical use of KCZ for attenuation of ovarian response to gonadotropin treatments. This study aimed to use the
Rebecca A Green et al.
The Journal of cell biology, 203(3), 505-520 (2013-11-13)
Abscission completes cytokinesis to form the two daughter cells. Although abscission could be organized from the inside out by the microtubule-based midbody or from the outside in by the contractile ring-derived midbody ring, it is assumed that midbody microtubules scaffold
Yukiko Nakase et al.
Journal of cell science, 126(Pt 17), 3972-3981 (2013-07-03)
Rheb GTPase and the Tsc1-Tsc2 protein complex, which serves as a GTPase-activating protein for Rheb, have crucial roles in the regulation of cell growth in response to extracellular conditions. In Schizosaccharomyces pombe, Rheb and Tsc1-Tsc2 regulate cell cycle progression, the
Zhongliang Zu et al.
Magnetic resonance in medicine, 69(3), 637-647 (2012-04-17)
In this study, we introduce a new method for amide proton transfer imaging based on chemical exchange rotation transfer. It avoids several artifacts that plague conventional chemical exchange saturation transfer approaches by creating label and reference scans based on varying
RNAi in cultured Drosophila cells.
Ling-Rong Kao et al.
Methods in molecular biology (Clifton, N.J.), 247, 443-457 (2004-01-07)
Elisa Cuevas et al.
Frontiers in cell and developmental biology, 3, 20-20 (2015-04-18)
Lin41/Trim71 is a heterochronic gene encoding a member of the Trim-NHL protein family, and is the original, genetically defined target of the microRNA let-7 in C. elegans. Both the LIN41 protein and multiple regulatory microRNA binding sites in the 3'
Baptiste Libé-Philippot et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(30), 7765-7774 (2017-07-15)
Many genetic forms of congenital deafness affect the sound reception antenna of cochlear sensory cells, the hair bundle. The resulting sensory deprivation jeopardizes auditory cortex (AC) maturation. Early prosthetic intervention should revive this process. Nevertheless, this view assumes that no
Narayana Pineda-Ramírez et al.
Antioxidants (Basel, Switzerland), 9(3) (2020-04-05)
This study aimed to compare the antioxidant activities of extracts obtained from three plant families and evaluate their therapeutic effect on strokes. Ethanol extracts were obtained from either the leaf or the aerial parts of plants of the families Annonaceae
Livia Goto-Silva et al.
Scientific reports, 9(1), 2433-2433 (2019-02-23)
Long-distance axonal trafficking plays a critical role in neuronal function and transport defects have been linked to neurodegenerative disorders. Various lines of evidence suggest that the small GTPase Rab5 plays a role in neuronal signaling via early endosomal transport. Here
Miroslaw Janowski et al.
PloS one, 9(2), e97631-e97631 (2014-06-12)
The purpose of the study was to evaluate the long-term clinical tracking of magnetically labeled stem cells after intracerebroventricular transplantation as well as to investigate in vitro feasibility for magnetic guidance of cell therapy within large fluid compartments. After approval
Pascal D Odermatt et al.
Nano letters, 15(8), 4896-4904 (2015-06-30)
Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating nanoscale structure to biological function of specific proteins
Toshio Nakamae et al.
Sports medicine, arthroscopy, rehabilitation, therapy & technology : SMARTT, 2(1), 5-5 (2010-02-16)
As the strategy for tissue regeneration using mesenchymal stem cells (MSCs) for transplantation, it is necessary that MSCs be accumulated and kept in the target area. To accumulate MSCs effectively, we developed a novel technique for a magnetic targeting system
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