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Shuangqi Fan et al.
Frontiers in microbiology, 10, 2962-2962 (2020-01-24)
Viruses have evolved many mechanisms to escape host antiviral responses. Previously, we found that classical swine fever virus (CSFV) infection induces autophagy using the autophagosome as a self-replication site, thereby evading the host immune response and promoting long-term infection. However
Els Pardon et al.
Nature protocols, 9(3), 674-693 (2014-03-01)
There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible
Kathryn A Ryan et al.
Nature communications, 12(1), 81-81 (2021-01-06)
There is a vital need for authentic COVID-19 animal models to enable the pre-clinical evaluation of candidate vaccines and therapeutics. Here we report a dose titration study of SARS-CoV-2 in the ferret model. After a high (5 × 106 pfu) and medium (5 × 104 pfu)
Dale Howe et al.
Methods in molecular biology (Clifton, N.J.), 445, 389-406 (2008-04-22)
Coxiella burnetii is a bacterial obligate intracellular pathogen that replicates within a spacious parasitophorous vacuole (PV) with lysosomal characteristics. The pathogen actively participates in the biogenesis of its PV by synthesizing proteins that mediate vesicular interactions. Both C. burnetii and
Izabela Ferreira Gontijo de Amorim et al.
PloS one, 6(11), e27679-e27679 (2011-12-06)
The aim of the present study was to investigate TLR2 expression in peripheral blood monocytes from dogs naturally infected with Leishmania (Leishmania) infantum to determine whether it correlates with CD11b/CD18 (CR3) expression, and to evaluate the potential of dogs as
Tüzer Kalkan et al.
Cell stem cell, 24(5), 785-801 (2019-04-30)
The gene regulatory network (GRN) of naive mouse embryonic stem cells (ESCs) must be reconfigured to enable lineage commitment. TCF3 sanctions rewiring by suppressing components of the ESC transcription factor circuitry. However, TCF3 depletion only delays and does not prevent
Daniela Sparvoli et al.
Current biology : CB, 28(5), 697-710 (2018-02-27)
In the endocytic pathway of animals, two related complexes, called CORVET (class C core vacuole/endosome transport) and HOPS (homotypic fusion and protein sorting), act as both tethers and fusion factors for early and late endosomes, respectively. Mutations in CORVET or
Sang Jun Han et al.
Genesis (New York, N.Y. : 2000), 47(12), 815-824 (2009-11-03)
Estrogen receptor alpha (ERalpha), plays essential roles in the female reproduction. To investigate the dynamic changes in ERalpha activity in vivo, we have developed an ER Alpha Activity Indicator (ERAAI) mouse. This ERAAI mouse harbors both a modified ERalpha Bacterial
Imène Mensi et al.
Bio-protocol, 7(2), e2111-e2111 (2017-01-20)
Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans
Jennifer E Dawson et al.
Nature communications, 11(1), 3146-3146 (2020-06-21)
Phosphorylation of intrinsically disordered eIF4E binding proteins (4E-BPs) regulates cap-dependent translation by weakening their ability to compete with eIF4G for eIF4E binding within the translation initiation complex. We previously showed that phosphorylation of T37 and T46 in 4E-BP2 induces folding
Barbara S Nelson et al.
Cancer & metabolism, 8, 1-1 (2020-01-08)
Metabolic programs in cancer cells are influenced by genotype and the tissue of origin. We have previously shown that central carbon metabolism is rewired in pancreatic ductal adenocarcinoma (PDA) to support proliferation through a glutamate oxaloacetate transaminase 1 (GOT1)-dependent pathway.
Yi-Chun Yen et al.
Frontiers in behavioral neuroscience, 9, 67-67 (2015-04-09)
Psychostimulants show therapeutic efficacy in the treatment of attention-deficit hyperactivity disorder (ADHD). It is generally assumed that they ameliorate ADHD symptoms via interfering with monoaminergic signaling. We combined behavioral pharmacology, neurochemistry and molecular analyses to identify mechanisms underlying the paradoxical
André F Maia et al.
Scientific data, 2, 150020-150020 (2015-05-20)
Kinesins are a superfamily of microtubule-based molecular motors that perform various transport needs and have essential roles in cell division. Among these, the kinesin-5 family has been shown to play a major role in the formation and maintenance of the
Andrés M Cardozo Gizzi et al.
Nature protocols, 15(3), 840-876 (2020-01-24)
Simultaneous observation of 3D chromatin organization and transcription at the single-cell level and with high spatial resolution may hold the key to unveiling the mechanisms regulating embryonic development, cell differentiation and even disease. We recently developed Hi-M, a technology that
Justin V Joseph et al.
PloS one, 10(12), e0145393-e0145393 (2015-12-25)
Glioblastoma (GBM) is a highly infiltrative brain tumor in which cells with properties of stem cells, called glioblastoma stem cells (GSCs), have been identified. In general, the dominant view is that GSCs are responsible for the initiation, progression, invasion and
Jeffrey Wimsatt et al.
BMC cancer, 16(1), 942-942 (2016-12-09)
Colorectal cancer is the second most common cause of cancer deaths for both men and women, and the third most common cause of cancer in the U.S. Toxicity of current chemotherapeutic agents for colorectal cancer, and emergence of drug resistance
Lycias Zembe et al.
PloS one, 6(10), e26096-e26096 (2011-10-25)
The genetic diversity of HIV-1 across the globe is a major challenge for developing an HIV vaccine. To facilitate immunogen design, it is important to characterize clusters of commonly targeted T-cell epitopes across different HIV clades. To address this, we
Alejandro Avilés-Reyes et al.
Bio-protocol, 5(7) (2015-04-05)
Bacterial glycoproteins are of increasing interest due to their abundance in nature and importance in health and infectious diseases. However, only a very small fraction of bacterial glycoproteins have been characterized and its post-translational modification machinery identified. While analysis of
Wenlin Du et al.
The American journal of pathology, 173(4), 1153-1164 (2008-09-06)
The Schwannian stroma in neuroblastomas is related to patient prognosis. There is debate surrounding the origin of Schwannian stroma in neuroblastomas: one theory is that the Schwann cells are derived from neoplastic cells, and the other is that they arise
Anna Bogstedt et al.
Journal of Alzheimer's disease : JAD, 46(4), 1091-1101 (2015-09-25)
Utilizing decision making biomarkers in drug development requires thorough assay validation. Special considerations need to be taken into account when monitoring biomarkers using immunoassays in the presence of therapeutic antibodies. We have developed robust and sensitive assays to assess target
Florent Arbogast et al.
Autophagy, 15(2), 280-294 (2018-09-11)
The involvement of macroautophagy/autophagy proteins in B-cell receptor (BCR) trafficking, although suspected, is not well understood. We show that ATG5 (autophagy related 5) contributes to BCR polarization after stimulation and internalization into LAMP1 (lysosomal-associated membrane protein 1)+ and major histocompatibility
Heidi Morales et al.
Biological procedures online, 10, 1-8 (2008-04-04)
Activation of lymphocytes in mammals is often quantified by measuring the amount of proliferation during the expansion phase of an immune response. Bromodeoxyuridine (BrdU) incorporation and carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution assays are some of the techniques widely used
Zhen Qiao et al.
Micromachines, 11(5) (2020-05-23)
Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients.
Olivia N Chuang et al.
Infection and immunity, 77(1), 539-548 (2008-10-29)
Aggregation substance proteins encoded by sex pheromone plasmids increase the virulence of Enterococcus faecalis in experimental pathogenesis models, including infectious endocarditis models. These large surface proteins may contain multiple functional domains involved in various interactions with other bacterial cells and
Michael Segel et al.
Nature, 573(7772), 130-134 (2019-08-16)
Ageing causes a decline in tissue regeneration owing to a loss of function of adult stem cell and progenitor cell populations1. One example is the deterioration of the regenerative capacity of the widespread and abundant population of central nervous system (CNS) multipotent
Yaroslava Karpova et al.
Cancers, 13(19) (2021-10-14)
Poly(ADP-ribose) polymerase 1 (PARP-1) and glycohydrolase (PARG) enzymes regulate chromatin structure, transcription activation, and DNA repair by modulating poly(ADP-ribose) (pADPr) level. Interest in PARP-1 inhibitors has soared recently with the recognition of their antitumor efficacy. We have shown that the
Sue Ellen Touma et al.
Biochemical pharmacology, 78(9), 1127-1138 (2009-06-25)
Retinoids, which include vitamin A (retinol) and metabolites such as retinoic acid, can inhibit tumor growth and reverse carcinogenesis in animal models of prostate cancer. We analyzed retinoid signaling and metabolism in the TRAMP (transgenic adenocarcinoma mouse prostate) model. We
Keiji Suzuki et al.
Nature protocols, 6(2), 134-139 (2011-02-05)
We describe a protocol for creating localized DNA double-strand breaks (DSBs) with minimal requirements that can be applied in cell biology and molecular biology. This protocol is based on the combination of 5-bromo-2'-deoxyuridine (BrdU) labeling and ultraviolet C (UVC) irradiation
Li Huang et al.
Autophagy, 15(3), 407-422 (2018-09-14)
Glucose produced from photosynthesis is a key nutrient signal regulating root meristem activity in plants; however, the underlying mechanisms remain poorly understood. Here, we show that, by modulating reactive oxygen species (ROS) levels, the conserved macroautophagy/autophagy degradation pathway contributes to
Remedios Castelló-Cros et al.
Methods in molecular biology (Clifton, N.J.), 522, 275-305 (2009-02-28)
It is increasingly recognized that interactions between cancer cells and their surrounding stroma are critical for promoting the growth and invasiveness of tumors. For example, cancer cells alter the topography and molecular composition of stromal extracellular matrix by increasing paracrine
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