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Preparation and Evaluation of PEGylated Poly-L-ornithine Complex as a Novel Absorption Enhancer
Yusuke Kamiya
Biologicals : Journal of the International Association of Biological Standardization (2017)
Keisuke Oda et al.
Drug metabolism and pharmacokinetics, 27(6), 570-578 (2012-04-19)
In this study, we elucidated the effect of poly(amino acid)s such as poly-L-ornithine (PLO) on FITC-insulin uptake in cultured alveolar type II epithelial cells, RLE-6TN. FITC-insulin uptake by RLE-6TN cells as well as its cell surface binding was markedly increased...
Florian Völlner et al.
Differentiation; research in biological diversity, 77(5), 433-441 (2009-04-28)
Human dental follicle cells (DFCs) derived from wisdom teeth are precursor cells for cementoblasts. In this study, we recognized that naïve DFCs express constitutively the early neural cell marker beta-III-tubulin. Interestingly, DFCs formed beta-III-tubulin-positive neurosphere-like cell clusters (NLCCs) on low-attachment...
Anna L Hillberg et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 101(2), 258-268 (2012-11-21)
DIABECELL® capsules comprise an inner core of alginate (Alg) coated with a polycationic polymer, poly-L-ornithine (PLO), designed as a stabilizing agent for strengthening the capsule wall, which is masked by an outer layer of biocompatible Alg. These polymeric microcapsules have...
Omaditya Khanna et al.
Journal of visualized experiments : JoVE, (66)(66), doi:10-doi:10 (2012-08-22)
Alginate-based materials have received considerable attention for biomedical applications because of their hydrophilic nature, biocompatibility, and physical architecture. Applications include cell encapsulation, drug delivery, stem cell culture, and tissue engineering scaffolds. In fact, clinical trials are currently being performed in...
Gustav Y Cederquist et al.
Cell stem cell, 27(1), 35-49 (2020-07-04)
Autism is a clinically heterogeneous neurodevelopmental disorder characterized by impaired social interactions, restricted interests, and repetitive behaviors. Despite significant advances in the genetics of autism, understanding how genetic changes perturb brain development and affect clinical symptoms remains elusive. Here, we...
Henrik Ring et al.
PloS one, 7(5), e36874-e36874 (2012-05-17)
GABA is more than the main inhibitory neurotransmitter found in the adult CNS. Several studies have shown that GABA regulates the proliferation of progenitor and stem cells. This work examined the effects of the GABA(A) receptor system on the proliferation...
Carl M Sellgren et al.
Nature neuroscience, 22(3), 374-385 (2019-02-06)
Synapse density is reduced in postmortem cortical tissue from schizophrenia patients, which is suggestive of increased synapse elimination. Using a reprogrammed in vitro model of microglia-mediated synapse engulfment, we demonstrate increased synapse elimination in patient-derived neural cultures and isolated synaptosomes....
Stefano Giovagnoli et al.
Pharmaceutical research, 27(2), 285-295 (2010-01-01)
To assess whether prevention of unexpected in vivo adverse inflammatory and immune responses to biohybrid organ grafts for the treatment of Type I Diabetes Mellitus (T1DM) is possible by superoxide dismutase and ketoprofen controlled release. Superoxide dismutase and ketoprofen-loaded polyester...
Pítia Flores Ledur et al.
Scientific reports, 10(1), 1218-1218 (2020-01-29)
Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research has revealed that astrocytes are targets of ZIKV. However, the consequences of ZIKV infection, especially to this cell type, remain largely unknown, particularly...
C G Thanos et al.
Journal of biomedical materials research. Part A, 83(1), 216-224 (2007-07-04)
Alginate encapsulation is one of the most widely used techniques for introducing cell-based therapeutics into the body. Numerous encapsulation methodologies exist, utilizing a variety of alginates, purification technologies, and unique polycationic membrane components. The stability of a conventional alginate formulation...
Vikram Babu Kasaragod et al.
Neuron, 101(4), 673-689 (2019-02-02)
The frontline anti-malarial drug artemisinin and its derivatives have also been implicated in modulating multiple mammalian cellular pathways, including the recent identification of targeting γ-aminobutyric acid type A receptor (GABAAR) signaling in the pancreas. Their molecular mechanism of action, however...
Vasiliki Mahairaki et al.
Tissue engineering. Part A, 17(5-6), 855-863 (2010-10-27)
The potential of human embryonic stem (ES) cells as experimental therapies for neuronal replacement has recently received considerable attention. In view of the organization of the mature nervous system into distinct neural circuits, key challenges of such therapies are the...
Yu-Xin Cui et al.
Journal of microencapsulation, 23(6), 663-676 (2006-11-23)
The viability, proliferation and insulin production of RIN-m5F cells when loaded into alginate beads to form a 3D culture system has been investigated. The mechanism of alginate cross-linking (calcium ions vs barium ions), the addition of poly(L-lysine) (PLL) and poly(L-ornithine...
Philipp Pottmeier et al.
Stem cells and development, 29(23), 1497-1509 (2020-10-13)
Human neural stem cells (hNSCs) have long been used as an in vitro model to study neurogenesis and as candidates for nervous system therapy. Many parameters have been considered when evaluating the success of transplantation, but sex of donor and...
Bella Koltun et al.
Nucleic acids research, 48(6), e32-e32 (2020-01-25)
In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were...
Electrophoretic deposition of chiral polymers and composites.
Wang Y, Pang X, Zhitomirsky I.
Colloids and Surfaces, B: Biointerfaces, 87, 505-509 (2011)
Michael Peitz et al.
STAR protocols, 1(1), 100038-100038 (2020-10-29)
This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. This approach is particularly...
Christopher S Morrow et al.
Cell stem cell, 26(4), 558-568 (2020-02-29)
Maintaining a healthy proteome throughout life is critical for proper somatic stem cell function, but the complexities of the stem cell response to increases in damaged or aggregated proteins remain unclear. Here we demonstrate that adult neural stem cells (NSCs)...
QiQi Zhou et al.
Gut, 65(5), 797-805 (2015-02-15)
Many patients with irritable bowel syndrome IBS not only have abdominal pain but also may suffer from visceral hypersensitivity and heighted visceral nociception. Moreover, IBS has few effective therapeutic agents and mechanisms of disease are unclear. Our goals were to...
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological...
Lorraine Iacovitti et al.
Brain research, 1127(1), 19-25 (2006-11-25)
Our ability to use human embryonic stem (hES) cells in cell replacement therapy for Parkinson's disease depends on the discovery of ways to simply and reliably differentiate a dopaminergic (DA) phenotype in these cells. Although several protocols exist for the...
Annie Kathuria et al.
Genome medicine, 12(1), 34-34 (2020-04-21)
Reprogramming human induced pluripotent stem cells (iPSCs) from somatic cells and generating three-dimensional brain organoids from these iPSCs provide access to live human neuronal tissue with disease-specific genetic backgrounds. Cerebral organoids were generated from iPSCs of eight bipolar disorder (BPI)...
Hikari Tanaka et al.
Nature communications, 11(1), 507-507 (2020-01-26)
The timing and characteristics of neuronal death in Alzheimer's disease (AD) remain largely unknown. Here we examine AD mouse models with an original marker, myristoylated alanine-rich C-kinase substrate phosphorylated at serine 46 (pSer46-MARCKS), and reveal an increase of neuronal necrosis...
Efficient DNA transfection of quiescent mammalian cells using poly-L-ornithine.
Y Dong et al.
Nucleic acids research, 21(3), 771-772 (1993-02-11)
Nicola Micali et al.
Cell reports, 31(5), 107599-107599 (2020-05-07)
Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and...
J P McQuilling et al.
Transplantation proceedings, 43(9), 3262-3264 (2011-11-22)
Severe hypoxia caused by a lack of vascular supply and an inability to retrieve encapsulated islets transplanted in the peritoneal cavity for biopsy and subsequent evaluation are obstacles to clinical application of encapsulation strategies for islet transplantation. We recently proposed...
Naiyan Wen et al.
International journal of oncology, 55(4), 879-895 (2019-09-06)
Bromodomain and extraterminal domain proteins, especially bromodomain‑containing protein 4 (Brd4), have recently emerged as therapeutic targets for several cancers, although the role and mechanism of Brd4 in glioblastoma multiforme (GBM) are unclear. In this study, we aimed to explore the...
Praneet Opanasopit et al.
International journal of pharmaceutics, 410(1-2), 161-168 (2011-03-15)
The aim of this research was to investigate the effect of cationic polypeptides mixed with chitosan (CS) on in vitro transfection efficiency and cytotoxicity in human cervical carcinoma cells (HeLa cells). The polypeptides/DNA complexes and ternary complexes (CS, polypeptides and...
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