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M Taskin et al.
Journal of applied microbiology, 111(4), 826-834 (2011-07-19)
Peptones are one of the most expensive constituents of microbial media. This study was undertaken to prepare the peptone from waste chicken feathers through a new process. The chemical analysis of chicken feather peptone (CFP) was performed. The ability of...
Aiqi Fang et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(32), 13225-13229 (2009-08-05)
In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy products, thus obviating...
Angela A Parry-Hanson et al.
Microbiological research, 165(7), 546-556 (2009-12-17)
Cross-protection of acid-adapted Escherichia coli O157:H7 against inimical stresses is mediated by the glucose-repressed sigma factor RpoS. However, many food systems in which E. coli O157:H7 occurs are complex and contain glucose. This study was aimed at investigating the contribution...
Mesut Taskin et al.
International journal of food sciences and nutrition, 63(5), 597-602 (2011-12-06)
This study was performed to investigate the usability of chicken feather hydrolysate (Chicken feather peptone (CFP)) as substrate for mycelial biomass and extracellular polysaccharides (EPS) production from edible mushroom Morchella esculenta. The ability of CFP to support biomass and EPS...
Saoussen Turki et al.
Bioresource technology, 100(10), 2724-2731 (2009-02-04)
In this work the effect of several organic nitrogen sources on lipase production in Yarrowia lipolytica LgX64.81 overproducing mutant was studied. Among them, tryptone and peptone showed the most prominent stimulatory effect. Interestingly, only tryptic and peptic casein digest were...
Michael Pistorino et al.
Biotechnology progress, 25(5), 1364-1371 (2009-07-28)
The recent use of heterologous hosts to produce natural products has shown significant potential, although limitations still exist regarding optimal production titers. In this study, we utilize micro-scale cultures and well-defined screening methods to identify key medium components that influence...
Filomena Silva et al.
Journal of microbiology and biotechnology, 19(11), 1408-1414 (2009-12-10)
The obtention of high yields of purified plasmid DNA is viewed as an essential issue to be considered towards efficient production of DNA vaccines and therapeutic plasmids. In this work, Escherichia coli DH5alpha bearing the pVAX1-LacZ plasmid was grown in...
Paulraj Kanmani et al.
Preparative biochemistry & biotechnology, 41(1), 40-52 (2011-01-14)
Statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic Enterococcus faecium MC13. Carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (OVAT) methods. In order to increase yield...
Robert H White
Journal of bacteriology, 192(20), 5437-5440 (2010-08-17)
N-Ethylglutamate (NEG) was detected in Escherichia coli BL21 cells grown on LB broth, and it was found to occur at a concentration of ∼4 mM in these cells under these conditions. The same cells grown on M9 glucose medium contained...
[Factors responsible for transition of the carnivorous fungus Duddingtonia flagrans from saprophytic to zootrophic type of nutrition ].
G G Anan'ko et al.
Mikrobiologiia, 80(2), 200-206 (2011-07-22)
Jigita Padhiar et al.
Pakistan journal of biological sciences : PJBS, 14(22), 1011-1018 (2012-04-21)
The present study was aimed at optimization, production and partial purification of lipases from Pseudomonas aeruginosa, Candida albicans and Aspergillus flavus. Various nutritional and physical parameters affecting lipase production such as carbon and nitrogen supplements, pH, temperature, agitation speed and...
Miguel Betancourt-Sanchez et al.
Microbiology (Reading, England), 155(Pt 9), 2895-2906 (2009-06-23)
In an in vitro model using HEp-2 cells treated with purified plasmid-encoded toxin (Pet), we have identified morphological changes characterized by cell rounding and detachment after toxin internalization; these changes progress to cell death. However, these effects have not yet...
V I Iliukhin et al.
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic], 52(1-2), 18-20 (2008-05-09)
Principles and procedure for rapid estimation of bacteria susceptibility to antibiotics on a glucose-tryptone medium with an indicator are described. The results of the tests with 50 microbial strains of 17 species showed practically complete identity to the results of...
D Esser et al.
Journal of proteome research, 11(10), 4823-4833 (2012-05-30)
Protein phosphorylation is known to occur in Archaea. However, knowledge of phosphorylation in the third domain of life is rather scarce. Homology-based searches of archaeal genome sequences reveals the absence of two-component systems in crenarchaeal genomes but the presence of...
Seok Hoon Hong et al.
Nature communications, 3, 613-613 (2012-01-05)
To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that...
H B Nielsen et al.
Environmental technology, 28(8), 905-914 (2007-09-21)
Two thermophilic continuously stirred tank reactors, R1 and R2, were subject to pulses of tryptone and ammonia. R1 was operated at an ammonia-N concentration of 3.0 g l(-1) and R2 was operated at an ammonia-N concentration of 1.7 g l(-1)....
G Csaba et al.
Acta microbiologica et immunologica Hungarica, 57(4), 393-399 (2010-12-25)
In order to approach their natural conditions, populations of Tetrahymena were kept in Losina-Losinky's salt solution for 1 h, than in the tryptone+yeast medium. During this time they were treated with histamine, serotonin or insulin, or with the combinations of...
P Shobharani et al.
International journal of food sciences and nutrition, 60 Suppl 6, 70-83 (2009-02-26)
Probiotic are microorganisms that, upon ingestion in adequate amounts, exert a beneficial effect on the host. In the present work, the potent probiotic Leuconostoc mesenteroides was used as a starter culture in the preparation of fermented milk beverage. The product...
Takashi Kuda et al.
Food chemistry, 134(4), 1719-1723 (2013-02-28)
Listeria monocytogenes causes listeriosis in humans, mainly through the consumption of ready-to-eat foods such as cheese. Immunocompromised persons, the elderly, and pregnant women and their fetuses or newborns are at the highest risk for the infection. We examined the effects...
Filomena Silva et al.
Applied microbiology and biotechnology, 93(6), 2571-2580 (2011-11-18)
In order to provide sufficient pharmaceutical-grade plasmid DNA material, it is essential to gain a comprehensive knowledge of the bioprocesses involved; so, the development of protocols and techniques that allow a fast monitoring of process performance is a valuable tool...
Eliane Frimmersdorf et al.
Environmental microbiology, 12(6), 1734-1747 (2010-06-18)
In addition to transcriptome and proteome studies, metabolome analysis represents a third complementary approach to identify metabolic pathways and adaptation processes. In order to elucidate basic principles of metabolic versatility of Pseudomonas aeruginosa, we investigated the metabolome profiles of two...
G Csaba et al.
Acta biologica Hungarica, 62(3), 228-234 (2011-08-16)
The aim of the experiments was to study the regulation of triiodothyronine (T3) production in the unicellular Tetrahymena. Untreated and troph-hormone treated specimen were prepared and in different timepoints T3 content was measured and compared by immunocytochemical flow cytometry. 0.1...
Sandra Borges et al.
Antonie van Leeuwenhoek, 101(3), 677-682 (2011-11-01)
The Group B Streptococcus (GBS, Streptococcus agalactiae) is an important cause of neonatal and maternal infection. GBS is a commensal organism of the lower gastrointestinal and vaginal tract. A frequent mode of neonatal infection is vertical transmission from pregnant women...
Neeraj Bala et al.
Journal of basic microbiology, 52(4), 383-389 (2011-11-05)
The incubation of whole Bacillus alcalophilus cells grown on a mineral supplemented medium (MSM) containing 1% (w/v) sucrose as carbon source, 1.2% (w/v) tryptone as nitrogen source at pH 6.5 and temperature 30 °C in 24 h kinetically resolved benzyl...
Priyadharshini Ramachandran et al.
Journal of microbiology and biotechnology, 23(3), 351-356 (2013-03-07)
A high beta-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from...
Shiv Shankar et al.
Applied biochemistry and biotechnology, 166(4), 1082-1094 (2011-12-29)
A novel laccase producing Basidiomycete Peniophora sp. (NFCCI-2131) was isolated from pulp and paper mill effluent. The optimal temperature and initial pH for laccase production by the isolate in submerged culture were found to be 30 and 4.6° C, respectively. Maltose...
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