Showing 1-30 of 593 results for "

P6354

"
null
Yunqi Lu et al.
Cancer research, 78(1), 64-74 (2017-11-29)
The MYC oncogene broadly promotes transcription mediated by all nuclear RNA polymerases, thereby acting as a positive modifier of global gene expression. Here, we report that MYC stimulates the transcription of DANCR, a long noncoding RNA (lncRNA) that is widely...
Mustapha Lhor et al.
Biochimica et biophysica acta, 1848(3), 878-885 (2014-12-30)
Retinol dehydrogenase 11 (RDH11) has been postulated to be anchored to membranes by means of its N-terminal segment in retinal pigment epithelial (RPE) cells where it participates to the visual cycle. The analysis of the primary sequence of RDH11 revealed...
Benoît Hastoy et al.
Scientific reports, 7(1), 2835-2835 (2017-06-08)
Exocytosis depends on cytosolic domains of SNARE proteins but the function of the transmembrane domains (TMDs) in membrane fusion remains controversial. The TMD of the SNARE protein synaptobrevin2/VAMP2 contains two highly conserved small amino acids, G100 and C103, in its...
Dominik Drabik et al.
Journal of fluorescence, 26(2), 661-669 (2015-12-24)
Fluorescence Correlation Spectroscopy (FCS) is a technique, which allows determination of the diffusion coefficient and concentration of fluorescent objects suspended in the solution. The measured parameter is the fluctuation of the fluorescence signal emitted by diffusing molecules. When 100 nm DOPC...
Nadia Jaber et al.
Journal of cell science, 129(23), 4424-4435 (2016-11-02)
The class III phosphoinositide 3-kinase (PI3K) Vps34 (also known as PIK3C3 in mammals) produces phosphatidylinositol 3-phosphate [PI(3)P] on both early and late endosome membranes to control membrane dynamics. We used Vps34-deficient cells to delineate whether Vps34 has additional roles in...
Patrick Walsh et al.
The Journal of biological chemistry, 289(15), 10419-10430 (2014-02-21)
The formation of fibrillar aggregates has long been associated with neurodegenerative disorders such as Alzheimer and Parkinson diseases. Although fibrils are still considered important to the pathology of these disorders, it is now widely understood that smaller amyloid oligomers are...
Frédéric Pincet et al.
PloS one, 11(7), e0158457-e0158457 (2016-07-09)
Fluorescence recovery after photobleaching (FRAP) is a standard method used to study the dynamics of lipids and proteins in artificial and cellular membrane systems. The advent of confocal microscopy two decades ago has made quantitative FRAP easily available to most...
Takuhiro Otosu et al.
Physical chemistry chemical physics : PCCP, 22(3), 1242-1249 (2019-12-19)
A supported lipid bilayer (SLB) is now an indispensable tool to analyze the dynamical properties of biomembranes. However, the effect of a solid support on the leaflet-specific lipid dynamics in a SLB remains elusive, which hampers the further application of...
Sayaka Katayama et al.
Biochimica et biophysica acta, 1828(9), 2134-2142 (2013-05-29)
Arginine-rich cell-penetrating peptides, including octaarginine (R8) and HIV-1 TAT peptides, have the ability to translocate through cell membranes and transport exogenous bioactive molecules into cells. Hydrophobic counteranions such as pyrenebutyrate (PyB) have been reported to markedly promote the membrane translocation...
Lucie Caillon et al.
Biochimica et biophysica acta, 1828(9), 2091-2098 (2013-05-28)
Human islet amyloid polypeptide (IAPP) forms amyloid fibrils in the pancreatic islets of patients suffering from type 2 diabetes mellitus (T2DM). The formation of IAPP fibrils has been shown to cause membrane damage which most likely is responsible for the...
Sabina M Maté et al.
Biochimica et biophysica acta, 1838(7), 1832-1841 (2014-03-13)
α-Hemolysin (HlyA) is a protein toxin, a member of the pore-forming Repeat in Toxin (RTX) family, secreted by some pathogenic strands of Escherichia coli. The mechanism of action of this toxin seems to involve three stages that ultimately lead to...
A Zhiliakov et al.
Biochimica et biophysica acta. Biomembranes, 1863(1), 183446-183446 (2020-08-24)
Membrane phase-separation is a mechanism that biological membranes often use to locally concentrate specific lipid species in order to organize diverse membrane processes. Phase separation has also been explored as a tool for the design of liposomes with heterogeneous and...
Neval Yilmaz et al.
ACS nano, 9(8), 7960-7967 (2015-07-30)
We examined the effect of a sphingomyelin (SM)-binding pore-forming toxin (PFT), lysenin, on the dynamics of a phase-separated membrane of SM, where SM formed liquid-ordered (Lo) domains with cholesterol (Chol) within a phosphatidylcholine-rich liquid-disordered (Ld) phase. We visualized the lysenin-induced...
Ziqing Wang et al.
Developmental cell, 43(2), 186-197 (2017-10-17)
Little is known about the cellular events promoting metastasis. We show that knockout of phospholipase D2 (PLD2), which generates the signaling lipid phosphatidic acid (PA), inhibits lung metastases in the mammary tumor virus (MMTV)-Neu transgenic mouse breast cancer model. PLD2...
Scott D Hansen et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(30), 15013-15022 (2019-07-07)
Phosphorylation reactions, driven by competing kinases and phosphatases, are central elements of cellular signal transduction. We reconstituted a native eukaryotic lipid kinase-phosphatase reaction that drives the interconversion of phosphatidylinositol-4-phosphate [PI(4)P] and phosphatidylinositol-4,5-phosphate [PI(4,5)P2] on membrane surfaces. This system exhibited bistability...
L H Moleiro et al.
Advances in colloid and interface science, 247, 543-554 (2017-07-25)
Membrane pores can significantly alter not only the permeation dynamics of biological membranes but also their elasticity. Large membrane pores able to transport macromolecular contents represent an interesting model to test theoretical predictions that assign active-like (non-equilibrium) behavior to the...
Zhen Huang et al.
Biochimica et biophysica acta. Biomembranes, 1861(11), 183050-183050 (2019-08-27)
Ordered lipid domains (rafts) are generally considered to be features of eukaryotic cells, but ordered lipid domains formed by cholesterol lipids have been identified in bacteria from the genus Borrelia, and similar cholesterol lipids exist in the bacterium Helicobacter pylori....
Helena L E Coker et al.
Biophysical journal, 116(6), 1085-1094 (2019-03-09)
Diffusion in cell membranes is not just simple two-dimensional Brownian motion but typically depends on the timescale of the observation. The physical origins of this anomalous subdiffusion are unresolved, and model systems capable of quantitative and reproducible control of membrane...
Wenjia Zhang et al.
Langmuir : the ACS journal of surfaces and colloids, 35(22), 7231-7241 (2019-05-24)
Interactions of nonionic poly(ethylene oxide)- b-poly(propylene oxide) (PEO-PPO) block copolymers, known as Pluronics or poloxamers, with cell membranes have been widely studied for a host of biomedical applications. Herein, we report how cholesterol within phosphatidylcholine (POPC) lipid bilayer liposomes and...
null
Evaluation of the effects of aldehydes on association colloid properties and oxidative stability in bulk oils.
Jo, et al.
Food Chemistry, 338, 127778-127778 (2020)
Michael C Owen et al.
The journal of physical chemistry. B (2019-08-10)
The great physiological relevance of glycolipids is being increasingly recognized, and glycolipid interactions have been shown to be central to cell-cell recognition, neuronal plasticity, protein-ligand recognition, and other important processes. However, detailed molecular-level understanding of these processes remains to be...
M A Bodescu et al.
Soft matter, 13(38), 6845-6851 (2017-08-23)
Melittin is a model system for the action of antimicrobial peptides which are potential candidates for novel antibiotics. We investigated the membrane lysis effect of melittin on phase-separated supported lipid bilayers (DOPC-DPPC) by atomic force microscopy. AFM images show that...
Yunching Chen et al.
Scientific reports, 7, 44123-44123 (2017-03-10)
Sorafenib is a RAF inhibitor approved for several cancers, including hepatocellular carcinoma (HCC). Inhibition of RAF kinases can induce a dose-dependent "paradoxical" upregulation of the downstream mitogen-activated protein kinase (MAPK) pathway in cancer cells. It is unknown whether "paradoxical" ERK...
Paola Ringhieri et al.
International journal of pharmaceutics, 473(1-2), 194-202 (2014-07-12)
Pure sterically stabilized micelles (SSM) of DSPE-PEG2000, and sterically stabilized mixed micelles (SSMM) containing PC or DOPC phospholipids (5, 10 or 20% mol/mol with respect to DSPE-PEG2000) are developed as delivery systems for the gold based cytotoxic drug Au(III)-dithiocarbamato complex...
Tsuyoshi Yoda et al.
Membranes, 10(12) (2020-12-24)
Ethyl caproate (EC) and isoamyl acetate (IA) are key flavor components of sake. Recently, attempts have been made to increase the content of good flavor components, such as EC and IA, in sake brewing. However, the functions of EC and...
Amrita Jain et al.
Journal of cell science, 130(2), 360-371 (2016-11-27)
A deregulation of ceramide biosynthesis in the endoplasmic reticulum (ER) is frequently linked to induction of mitochondrial apoptosis. Although in vitro studies suggest that ceramides might initiate cell death by acting directly on mitochondria, their actual contribution to the apoptotic...
Francesca Susa et al.
Cryobiology, 98, 46-56 (2021-01-06)
In the last decades, liposomes acquired a striking success in the biomedical field thanks to their biocompatibility and drug delivery ability. Many liposomal drug formulations have been already approved by the Food and Drug Administration (FDA) and used for the...
Alina A Pashkovskaya et al.
Biophysical journal, 114(9), 2142-2151 (2018-05-10)
Long-chain free fatty acids (FFAs) play an important role in several physiological and pathological processes such as lipid fusion, adjustments of membrane permeability and fluidity, and the regulation of enzyme and protein activities. FFA-facilitated membrane proton transport (flip-flop) and FFA-dependent...