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P6354

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Hilal Yılmaz et al.
Protein expression and purification, 173, 105664-105664 (2020-05-08)
In this study, effect of affinity tags, Histidine (His) and Glutathione-S-Transferase (GST), on the activity of halophilic aquaporin was analyzed. The gene coding for H. elongata aquaporin was cloned into pET28a vector and expressed in E. coli BL21 successfully. Stopped...
Eun-Ji Jang et al.
Drug delivery, 24(1), 1587-1597 (2017-10-17)
Studies have shown that insertion of oleic acid into lipid bilayers can modulate the membrane properties of liposomes so as to improve their function as drug carriers. Considering that 2-hydroxyoleic acid (2OHOA), a potential antitumor agent currently undergoing clinical trials...
Ellen K Wasan et al.
Vaccine, 37(11), 1503-1515 (2019-02-12)
We previously developed an highly efficacious combination adjuvant comprised of innate defense regulator (IDR)-1002 peptide, poly(I:C) and polyphosphazene (TriAdj). Here we aimed to design and test the in vivo efficacy of a mucoadhesive nasal formulation of this adjuvant. To determine...
Koki Kamiya et al.
Micromachines, 12(2) (2021-02-04)
We determined the properties of fusion between large unilamellar vesicles (LUVs) and the lipid monolayer by measuring the fluorescence intensity of rhodamine-conjugated phospholipids in cell-sized lipid vesicles. The charge of LUVs (containing cationic lipids) and lipid droplets (containing anionic lipids)...
Xiaohui Liu et al.
The Journal of cell biology, 218(4), 1408-1421 (2019-02-21)
Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in...
M A Bodescu et al.
Soft matter, 13(38), 6845-6851 (2017-08-23)
Melittin is a model system for the action of antimicrobial peptides which are potential candidates for novel antibiotics. We investigated the membrane lysis effect of melittin on phase-separated supported lipid bilayers (DOPC-DPPC) by atomic force microscopy. AFM images show that...
Ziqing Wang et al.
Developmental cell, 43(2), 186-197 (2017-10-17)
Little is known about the cellular events promoting metastasis. We show that knockout of phospholipase D2 (PLD2), which generates the signaling lipid phosphatidic acid (PA), inhibits lung metastases in the mammary tumor virus (MMTV)-Neu transgenic mouse breast cancer model. PLD2...
Francesca Susa et al.
Cryobiology, 98, 46-56 (2021-01-06)
In the last decades, liposomes acquired a striking success in the biomedical field thanks to their biocompatibility and drug delivery ability. Many liposomal drug formulations have been already approved by the Food and Drug Administration (FDA) and used for the...
Shalini Singh et al.
Scientific reports, 7(1), 212-212 (2017-03-18)
The objective of the present study is the investigation of possibilities for boosting peptide anti-inflammatory effects by tryptophan end-tagging, including identification of underlying mechanisms for this. In doing so, effects of tryptophan end-tagging of KYE21 (KYEITTIHNLFRKLTHRLFRR), a peptide derived from...
Romina F Vázquez et al.
Colloids and surfaces. B, Biointerfaces, 158, 76-83 (2017-07-07)
Uropathogenic strains of Escherichia coli produce virulence factors, such as the protein toxin alpha-hemolysin (HlyA), that enable the bacteria to colonize the host and establish an infection. HlyA is synthetized as a protoxin (ProHlyA) that is transformed into the active...
Zhen Huang et al.
Biochimica et biophysica acta. Biomembranes, 1861(11), 183050-183050 (2019-08-27)
Ordered lipid domains (rafts) are generally considered to be features of eukaryotic cells, but ordered lipid domains formed by cholesterol lipids have been identified in bacteria from the genus Borrelia, and similar cholesterol lipids exist in the bacterium Helicobacter pylori....
Paola Ringhieri et al.
International journal of pharmaceutics, 473(1-2), 194-202 (2014-07-12)
Pure sterically stabilized micelles (SSM) of DSPE-PEG2000, and sterically stabilized mixed micelles (SSMM) containing PC or DOPC phospholipids (5, 10 or 20% mol/mol with respect to DSPE-PEG2000) are developed as delivery systems for the gold based cytotoxic drug Au(III)-dithiocarbamato complex...
Tsuyoshi Yoda et al.
Membranes, 10(12) (2020-12-24)
Ethyl caproate (EC) and isoamyl acetate (IA) are key flavor components of sake. Recently, attempts have been made to increase the content of good flavor components, such as EC and IA, in sake brewing. However, the functions of EC and...
Helena L E Coker et al.
Biophysical journal, 116(6), 1085-1094 (2019-03-09)
Diffusion in cell membranes is not just simple two-dimensional Brownian motion but typically depends on the timescale of the observation. The physical origins of this anomalous subdiffusion are unresolved, and model systems capable of quantitative and reproducible control of membrane...
Wenjia Zhang et al.
Langmuir : the ACS journal of surfaces and colloids, 35(22), 7231-7241 (2019-05-24)
Interactions of nonionic poly(ethylene oxide)- b-poly(propylene oxide) (PEO-PPO) block copolymers, known as Pluronics or poloxamers, with cell membranes have been widely studied for a host of biomedical applications. Herein, we report how cholesterol within phosphatidylcholine (POPC) lipid bilayer liposomes and...
Lianhua Shen et al.
Best practice & research. Clinical rheumatology, 32(4), 572-590 (2019-06-09)
β2-Glycoprotein I (β2GPI) is a highly-glycosylated plasma protein composed of five homologous domains which regulates coagulation, fibrinolysis, and/or angiogenesis by interacting to negatively charged hydrophobic molecules and/or with plasminogen and its metabolites. The present study focused on structural and functional characterization...
Heather E Findlay et al.
Scientific reports, 6, 39349-39349 (2016-12-21)
Nature encapsulates reactions within membrane-bound compartments, affording sequential and spatial control over biochemical reactions. Droplet Interface Bilayers are evolving into a valuable platform to mimic this key biological feature in artificial systems. A major issue is manipulating flow across synthetic...
Alina A Pashkovskaya et al.
Biophysical journal, 114(9), 2142-2151 (2018-05-10)
Long-chain free fatty acids (FFAs) play an important role in several physiological and pathological processes such as lipid fusion, adjustments of membrane permeability and fluidity, and the regulation of enzyme and protein activities. FFA-facilitated membrane proton transport (flip-flop) and FFA-dependent...
Jaehyoun Lee et al.
Biochemistry, 56(7), 977-985 (2017-01-28)
Nucleosomes impose physical barriers to DNA-templated processes, playing important roles in eukaryotic gene regulation. DNA is packaged into nucleosomes by histone proteins mainly through strong electrostatic interactions that can be modulated by various post-translational histone modifications. Investigating the dynamics of...
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Masanao Kinoshita et al.
Biochimica et biophysica acta, 1838(5), 1372-1381 (2014-01-29)
Recently, DHSM, a minor constituent in naturally occurring SMs, was indicated to form a raft-like ordered phase more effectively than a naturally occurring form of SM because DHSM has greater potential to induce the intermolecular hydrogen bond. In order to...
Renee J Tran et al.
The journal of physical chemistry. B, 123(22), 4673-4687 (2019-05-16)
An extensive investigation into the initial association of HIV-1 Gag with lipid membranes was conducted with second harmonic generation. The roles of the lipid phase, phospholipid 1,2-dioleoyl- sn-glycero-3-phospho-(1-myo-inositol-4,5-bisphosphate) [PI(4,5)P2], the presence of the myristoyl group on Gag, the C-terminus of...
C Favard et al.
Science advances, 5(10), eaaw8651-eaaw8651 (2019-10-17)
HIV-1 Gag protein assembles at the plasma membrane of infected cells for viral particle formation. Gag targets lipids, mainly PI(4,5)P2, at the inner leaflet of this membrane. Here, we address the question whether Gag is able to trap specifically PI(4,5)P2...
Fumi Sato-Kaneko et al.
Frontiers in immunology, 11, 1207-1207 (2020-07-09)
The limited efficacy of seasonal influenza vaccines is usually attributed to ongoing variation in the major antigenic targets for protective antibody responses including hemagglutinin (HA) and neuraminidase (NA). Hence, vaccine development has largely focused on broadening antigenic epitopes to generate...
Yunching Chen et al.
Scientific reports, 7, 44123-44123 (2017-03-10)
Sorafenib is a RAF inhibitor approved for several cancers, including hepatocellular carcinoma (HCC). Inhibition of RAF kinases can induce a dose-dependent "paradoxical" upregulation of the downstream mitogen-activated protein kinase (MAPK) pathway in cancer cells. It is unknown whether "paradoxical" ERK...
A Zhiliakov et al.
Biochimica et biophysica acta. Biomembranes, 1863(1), 183446-183446 (2020-08-24)
Membrane phase-separation is a mechanism that biological membranes often use to locally concentrate specific lipid species in order to organize diverse membrane processes. Phase separation has also been explored as a tool for the design of liposomes with heterogeneous and...
Neval Yilmaz et al.
ACS nano, 9(8), 7960-7967 (2015-07-30)
We examined the effect of a sphingomyelin (SM)-binding pore-forming toxin (PFT), lysenin, on the dynamics of a phase-separated membrane of SM, where SM formed liquid-ordered (Lo) domains with cholesterol (Chol) within a phosphatidylcholine-rich liquid-disordered (Ld) phase. We visualized the lysenin-induced...
Magda Przybyło et al.
Biochimica et biophysica acta, 1859(8), 1301-1309 (2017-05-04)
Organotin compounds, being biologically active, affect a variety of cellular functions due to their ability to accumulate in and penetrate biological membranes. These compounds influence the distribution of electrostatic charges, alter organization, disrupt molecular dynamics and change mechanical properties of...
Keishi Suga et al.
European biophysics journal : EBJ, 45(1), 55-62 (2015-09-20)
The hammerhead ribozyme (HHR) is one of smallest catalytic RNAs, composed of a catalytic core and three stems; it undergoes self-cleavage in the presence of divalent magnesium ions (Mg(2+)) or other cations. It is hypothesized that the function and metabolism...
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