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Shi Yun Leong et al.
PLoS pathogens, 11(3), e1004686-e1004686 (2015-03-10)
Human Enterovirus 71 (EV71) commonly causes Hand, Foot and Mouth Disease in young children, and occasional occurrences of neurological complications can be fatal. In this study, a high-throughput cell-based screening on the serine/threonine kinase siRNA library was performed to identify
Yashpal S Chhonker et al.
Fitoterapia, 97, 34-42 (2014-05-21)
A sensitive, selective, and rapid high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the quantification of rohitukine in rat plasma. HPLC was performed using a Symmetry-Shield C18 (5 μ, 4.6 × 150 mm) column, and isocratic elution with
Tyson Smyth et al.
Bioconjugate chemistry, 25(10), 1777-1784 (2014-09-16)
A method for conjugation of ligands to the surface of exosomes was developed using click chemistry. Copper-catalyzed azide alkyne cycloaddition (click chemistry) is ideal for biocojugation of small molecules and macromolecules to the surface of exosomes, due to fast reaction
Monika Johansson et al.
Journal of pharmaceutical and biomedical analysis, 100, 215-229 (2014-08-30)
An effective screening procedure to identify and quantify active pharmaceutical substances in suspected illegal medicinal products is described. The analytical platform, consisting of accurate mass determination with liquid chromatography time-of-flight mass spectrometry (LC-QTOF-MS) in combination with nuclear magnetic resonance (NMR)
Yanli Zhao et al.
Journal of clinical pharmacology, 54(10), 1180-1187 (2014-04-29)
The cephalosporin antibiotic ceftriaxone was evaluated as a potential therapeutic agent for the treatment of amyotrophic lateral sclerosis (ALS). The pharmacokinetics (PK) of ceftriaxone in plasma and cerebrospinal fluid (CSF) were investigated in 66 participants in a previously reported clinical
Christine Burkard et al.
PLoS pathogens, 10(11), e1004502-e1004502 (2014-11-07)
Enveloped viruses need to fuse with a host cell membrane in order to deliver their genome into the host cell. While some viruses fuse with the plasma membrane, many viruses are endocytosed prior to fusion. Specific cues in the endosomal
Trygve Brautaset et al.
Applied microbiology and biotechnology, 74(1), 22-34 (2007-01-12)
Amino acids are among the major products in biotechnology in both volume and value, and the global market is growing. Microbial fermentation is the dominant method used for industrial production, and today the most important microorganisms used are Corynebacteria utilizing
Ana-Maria Dragoi et al.
Infection and immunity, 82(11), 4447-4457 (2014-08-13)
Shigella flexneri is an intracellular pathogen that disseminates in the intestinal epithelium by displaying actin-based motility. We found that although S. flexneri displayed comparable actin-based motilities in the cytosols of HeLa229 and HT-29 epithelial cell lines, the overall dissemination process
Sonal et al.
PLoS genetics, 10(9), e1004614-e1004614 (2014-09-19)
The epidermis is a stratified epithelium, which forms a barrier to maintain the internal milieu in metazoans. Being the outermost tissue, growth of the epidermis has to be strictly coordinated with the growth of the embryo. The key parameters that
Tsutomu Ishi-i et al.
Organic & biomolecular chemistry, 13(6), 1818-1828 (2014-12-17)
In this study, we report a preparation and an aggregate emission behavior of an amphiphilic donor-acceptor dye, which is composed of a triphenylamine-benzothiadiazole donor-acceptor chromophore and two water-soluble hexa(ethylene glycol) chains. The dye is strongly fluorescent in nonpolar solutions such
Meagan J Bemer et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 20(10), 1544-1552 (2014-06-14)
Mycophenolic acid, the active metabolite of mycophenolate mofetil (MMF), inhibits inosine monophosphate dehydrogenase (IMPDH) activity. IMPDH is the rate-limiting enzyme involved in de novo synthesis of guanosine nucleotides and catalyzes the oxidation of inosine 5'-monophosphate to xanthosine 5'-monophosphate (XMP). We
Michelle Franz-Montan et al.
Journal of liposome research, 25(1), 11-19 (2014-05-09)
To characterize liposomal-lidocaine formulations for topical use on oral mucosa and to compare their in vitro permeation and in vivo anesthetic efficacy with commercially available lidocaine formulations. Large unilamellar liposomes (400 nm) containing lidocaine were prepared using phosphatidylcholine, cholesterol, and α-tocoferol
Nima Sepehri et al.
International journal of pharmaceutics, 471(1-2), 485-497 (2014-06-01)
SN38 (7-ethyl-10-hydroxyl camptothecin), a potent metabolite of irinotecan, has been considered as an anticancer candidate. Its clinical development has been hampered due to its poor solubility. As a result, SN38 loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) was developed in
Joanne A Lasrado et al.
Regulatory toxicology and pharmacology : RTP, 71(2), 213-224 (2014-12-21)
A proprietary dry spearmint extract containing 15.4% rosmarinic acid was assessed in a 90-day study with Sprague-Dawley rats that were gavaged at 0, 422 (low), 844 (mid), or 1948 (high) mg dry spearmint extract/kg bw/day, (equivalent to 0, 65, 130
Juan Qin et al.
Biomedical chromatography : BMC, 29(2), 261-267 (2014-06-14)
A simple, specific and sensitive LC-MS/MS method was developed and validated for the determination of mesalazine in beagle dog plasma. The plasma samples were prepared by protein precipitation, then the separation of the analyte was achieved on a Waters Spherisorb
Aimin Qiao et al.
Fitoterapia, 98, 137-142 (2014-08-05)
Sour jujube is a common fruit and traditional medicine in China. Bioactivity-guided fractionation of sour jujube was used to determine the chemical identity of potent antiproliferative and antioxidant constituents. Four novel ursane-type triterpenoids, together with 8 known were isolated and
Anna Laura Capriotti et al.
Analytical and bioanalytical chemistry, 407(6), 1705-1719 (2014-12-30)
A sensitive liquid chromatography/electrospray ionization-tandem mass spectrometry method for the determination of free and conjugated estrogens in cow milk is described. Milk samples were diluted with water and then extracted and cleaned up by solid-phase extraction using graphitized carbon black
Bharathi Avula et al.
Journal of AOAC International, 98(1), 13-21 (2015-04-11)
Alkaloids have been reported to be the major physiologically active constituents in Mitragyna. An analytical method was developed to provide an alternative, fast method for characterization of alkaloids from various M. speciosa samples. The separation was achieved using an RP
Alexandre Seillier et al.
Pharmacology, biochemistry, and behavior, 124, 153-159 (2014-06-10)
The biological actions of the endocannabinoids anandamide and 2-arachidonoyl glycerol (2-AG) are terminated by enzymatic hydrolysis of these lipids via fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL), respectively. While several selective FAAH inhibitors have been developed and characterized
Panpicha Sattasathuchana et al.
BMC veterinary research, 11, 5-5 (2015-01-18)
3-Bromotyrosine (3-BrY) is a stable product of eosinophil peroxidase and may serve as a marker of eosinophil activation. A gas chromatography/mass spectrometry method to measure 3-BrY concentrations in serum from dogs has recently been established and analytically validated. The aims
Vivian Caetano Bochi et al.
Food chemistry, 176, 234-243 (2015-01-28)
Ceylon gooseberry is a deep-purple exotic berry that is being produced in Brazil with great market potential. This work aimed to determine major phenolic compounds in this specie by HPLC-PDA-ESI/MS. Samples were collected in two different seasons. Pulp and skin
Maria M Posada et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(3), 325-334 (2014-12-17)
Pemetrexed, an anionic anticancer drug with a narrow therapeutic index, is eliminated mainly by active renal tubular secretion. The in vitro to in vivo extrapolation approach used in this work was developed to predict possible drug-drug interactions (DDIs) that may
Gimena dos Santos et al.
Nature communications, 6, 6574-6574 (2015-03-13)
Activation of the NLRP3 inflammasome and subsequent maturation of IL-1β have been implicated in acute lung injury (ALI), resulting in inflammation and fibrosis. We investigated the role of vimentin, a type III intermediate filament, in this process using three well-characterized
P Sivaperumal et al.
Food chemistry, 168, 356-365 (2014-08-31)
A multiresidue method, based on the sample preparation by solid-phase extraction cartridges and detection by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOF-MS), was used for the analysis of 60 pesticides in vegetable and fruit samples. Quantitation by UHPLC/TOF-MS is accomplished by
Pearse McCarron et al.
Analytical and bioanalytical chemistry, 407(11), 2985-2996 (2014-10-23)
Azaspiracids (AZAs) are lipophilic biotoxins produced by marine algae that can contaminate shellfish and cause human illness. The European Union (EU) regulates the level of AZAs in shellfish destined for the commercial market, with liquid chromatography-mass spectrometry (LC-MS) being used
Xuemei Zhang et al.
The Journal of pharmacy and pharmacology, 67(2), 160-169 (2015-01-06)
It is challenging to deliver the therapeutic drug effectively to the posterior ocular disease location with optimized exposure and long-term effects when treating proliferative vitreoretinopathy (PVR). The objective of this study is to develop a novel biodegradable and long-acting ocular
Sabiha Karim et al.
Acta poloniae pharmaceutica, 71(3), 463-468 (2014-10-01)
The objective of this study was to assess the bioequivalence between the omeprazole laboratory based formulation and the commercial formulation, Zimor Rubio, Spain, considered as reference formulation. The experiment was carried out according to a 2-period, 2-sequence crossover design with
Chinnasamy Gandhimathi et al.
Materials science & engineering. C, Materials for biological applications, 49, 776-785 (2015-02-18)
Mimicking native extracellular matrix with electrospun porous bio-composite nanofibrous scaffolds has huge potential in bone tissue regeneration. The aim of this study is to fabricate porous poly(l-lactic acid)-co-poly-(ε-caprolactone)/silk fibroin/ascorbic acid/tetracycline hydrochloride (PLACL/SF/AA/TC) and nanohydroxyapatite (n-HA) was deposited by calcium-phosphate dipping
Ninette Abou Mrad et al.
Analytical chemistry, 86(16), 8391-8399 (2014-07-16)
This contribution presents an original analytical system for studying volatile organic compounds (VOC) coming from the heating and/or irradiation of interstellar/cometary ice analogues (VAHIIA system) through laboratory experiments. The VAHIIA system brings solutions to three analytical constraints regarding chromatography analysis:
Fahad I Al-Jenoobi et al.
Pharmaceutical biology, 53(2), 296-300 (2014-09-23)
Herb-drug interactions are a serious problem especially for drugs with a narrow therapeutic index, taking into consideration that herbal medicines are commonly used in various parts of the world. The present study investigates the effect of fenugreek, garden cress, and
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