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Cecilie T Vangsøe et al.
Journal of the science of food and agriculture, 101(5), 1910-1919 (2020-09-09)
Cereal co-products rich in dietary fibres are increasingly used in animal feed. The high fibre content decreases the digestibility and reduces the nutrient and energy availability, resulting in lower nutritive value. Therefore, this study investigated the ability of two carbohydrase
Manuel Nieto-Domínguez et al.
Journal of agricultural and food chemistry, 65(48), 10526-10533 (2017-11-10)
The eco-friendly synthesis of non-natural glycosides from different phenolic antioxidants was carried out using a fungal β-xylosidase to evaluate changes in their bioactivities. Xylosides from hydroquinone and catechol were successfully formed, although the best results were obtained for hydroxytyrosol, the
Maria Romero-Fernández et al.
Bioresource technology, 266, 249-258 (2018-07-10)
The present study focuses on the development and optimization of a packed-bed reactor (PBR) for continuous production of xylooligosaccharides (XOS) from xylan. For this purpose, three different methacrylic polymer-based supports (Relizyme R403/S, Purolite P8204F and Purolite P8215F) activated with glyoxyl
Nuno Torres Faria et al.
New biotechnology, 49, 137-143 (2018-11-14)
Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their
Jong Min Oh et al.
Journal of microbiology and biotechnology, 28(5), 757-764 (2018-04-12)
A cellulolytic fungus, YDJ14, was isolated from compost and identified as an Aspergillus sp. strain. Three extracellular β-glucosidases, BGL-A1, BGL-A2, and BGL-A3, were separated using ultrafiltration, ammonium sulfate fractionation, and High-Q chromatography. The molecular masses of the three enzymes were
Joana R Costa et al.
Food & function, 10(4), 1856-1869 (2019-04-06)
Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health. The objective of this work
Tianwang Xu et al.
Journal of agricultural and food chemistry, 66(16), 4182-4188 (2018-04-11)
To simplify purification and improve heat tolerance of a thermostable β-xylosidase (ThXylC), a short ELK16 peptide was attached to its C-terminus, which is designated as ThXylC-ELK. Wild-type ThXylC was normally expressed in soluble form. However, ThXylC-ELK assembled into aggregates with
Sophie C Brandt et al.
Frontiers in microbiology, 11, 2154-2154 (2020-10-20)
A prerequisite for the transition toward a biobased economy is the identification and development of efficient enzymes for the usage of renewable resources as raw material. Therefore, different xylanolytic enzymes are important for efficient enzymatic hydrolysis of xylan-heteropolymers. A powerful
Stefan Jenkins et al.
Frontiers in microbiology, 8, 2618-2618 (2018-01-10)
Exometabolomics enables analysis of metabolite utilization of low molecular weight organic substances by soil bacteria. Environmentally-based defined media are needed to examine ecologically relevant patterns of substrate utilization. Here, we describe an approach for the construction of defined media using
Katarína Šuchová et al.
Enzyme and microbial technology, 134, 109484-109484 (2020-02-12)
Xylanases of the GH30 family are grouped to subfamilies GH30-7 and GH30-8. The GH30-8 members are of bacterial origin and well characterized, while the GH30-7 members are from fungal sources and their properties are quite diverse. Here, a heterologous expression
M C Jonathan et al.
Biotechnology for biofuels, 10, 112-112 (2017-05-05)
Corn stover is lignocellulosic biomass that has potential to be used as raw material for bioethanol production. In the current research, dilute ammonia pretreatment was used to improve the accessibility of corn stover carbohydrates to subsequently added hydrolytic enzymes. Some
Nuntawat Khat-Udomkiri et al.
AMB Express, 8(1), 115-115 (2018-07-18)
Rice husk (RH) is the major agricultural waste obtained during rice hulling process, which can be a sustainable source of xylooligosaccharide (XOS). The current study deals with the production of XOS from Thai rice husk using alkaline pretreatment and enzyme
María de Castro et al.
Journal of integrative plant biology, 59(7), 475-495 (2017-05-06)
As a consequence of the habituation to low levels of dichlobenil (DCB), cultured maize cells presented an altered hemicellulose cell fate with a lower proportion of strongly wall-bound hemicelluloses and an increase in soluble extracellular polymers released into the culture
Tomohiko Matsuzawa et al.
Applied microbiology and biotechnology, 101(2), 633-646 (2016-08-26)
MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a
Bing Guo et al.
Marine drugs, 11(4), 1173-1187 (2013-04-10)
Marine xylanases are rather less studied compared to terrestrial xylanases. In this study, a new xylanase gene, xynB, was cloned from the marine bacterium, Glaciecola mesophila KMM241, and expressed in Escherichia coli. xynB encodes a multi-domain xylanase XynB of glycoside
Hao Shi et al.
Biotechnology for biofuels, 6(1), 26-26 (2013-02-20)
Xylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent attention as powerful enzyme as well as
Yaping Gao et al.
Plant physiology, 173(1), 470-481 (2016-11-20)
Acetylation is a ubiquitous modification on cell wall polymers, which play a structural role in plant growth and stress defenses. However, the mechanisms for how crop plants accomplish cell wall polymer O-acetylation are largely unknown. Here, we report on the
T Dale et al.
British poultry science, 61(4), 471-481 (2020-07-21)
1. This study quantified xylanase-induced changes in soluble monosaccharides, xylooligosaccharides (XOS) and volatile fatty acid (VFA) contents of the different sections of the gastrointestinal tract (GIT) and whether these were related to altered bird performance. 2. An in vitro digestion
Tomohiko Matsuzawa et al.
FEBS open bio, 9(1), 92-100 (2019-01-18)
Xyloglucan is one of the major polysaccharides found in the plant cell wall and seeds. Owing to its complex branched structure, several different hydrolases are required to degrade it. Isoprimeverose-producing enzymes (IPase) are unique among the glycoside hydrolase 3 family
Nasim Najjarzadeh et al.
Molecules (Basel, Switzerland), 25(24) (2020-12-17)
Xylan is one of the most abundant carbohydrates on Earth. Complete degradation of xylan is achieved by the collaborative action of endo-β-1,4-xylanases and β-d-xylosidases and a number of accessories enzymes. In filamentous fungi, the xylanolytic system is controlled through induction
Chuannan Long et al.
3 Biotech, 8(1), 26-26 (2017-12-28)
The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated β-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in
Wen-Kang Huo et al.
International journal of biological macromolecules, 117, 1343-1351 (2017-12-05)
The rice xylanase inhibitor gene, rixi, was cloned from rice genome. The open reading frame of rixi was 915 bp and encoded 304 amino acids with the theoretical molecular mass of 33.9 kDa. The rixi was inserted into the new-type expression vector
Feng Zhang et al.
Chemistry, an Asian journal, 12(3), 324-331 (2016-11-25)
This work illustrates several theoretical fundamentals for the application of THz vibrational spectroscopy to molecular characterization in the solid state using two different types of saccharide systems as examples. Four subjects have been specifically addressed: (1) the qualitative differences in the
Maria Angélica F Pereira et al.
Bioprocess and biosystems engineering, 44(2), 297-306 (2020-09-20)
The deconstruction of banana peel for carbohydrate recovery was performed by sequential treatment (acid, alkaline, and enzymatic). The pretreatment with citric acid promoted the extraction of pectin, resulting in a yield of 8%. In addition, xylose and XOS, 348.5 and
André Busch et al.
PloS one, 12(8), e0184305-e0184305 (2017-08-31)
Hemicelluloses, such as xyloglucan, xylan and mannans, consist of a heterogeneous array of plant-derived polysaccharides that form the plant cell wall. These polysaccharides differ from each other in their structure and physiochemical properties, but they share a β-(1,4)-linked sugar backbone.
Maria Louise Leth et al.
Nature microbiology, 3(5), 570-580 (2018-04-04)
Metabolism of dietary glycans is pivotal in shaping the human gut microbiota. However, the mechanisms that promote competition for glycans among gut commensals remain unclear. Roseburia intestinalis, an abundant butyrate-producing Firmicute, is a key degrader of the major dietary fibre xylan.
Ramanan Sekar et al.
Bioresource technology, 218, 1133-1139 (2016-07-30)
The aim of this work was to develop a microbially-driven Fenton reaction that fragments cellulose and hemicellulose, degrades cellodextrins and xylodextrins, and produces short-chain oligosaccharides and monomeric sugars in a single bioreactor. The lignocellulose degradation system operates at neutral pH
Yingying Zheng et al.
Journal of structural biology, 193(3), 206-211 (2016-01-01)
The cold-adapted xylanases can catalyze at low temperature and hold great potential in food industry applications. Here we describe the first crystal structure of a cold-adapted glycoside hydrolase (GH) family 10 xylanase XynGR40 and its complex with xylobiose at 2.15
Douglas B Jordan et al.
Applied biochemistry and biotechnology, 182(1), 250-260 (2016-11-18)
Divalent metal-activated glycoside hydrolase family 43 (GH43) β-xylosidases have been found to have high k
Daniel Wefers et al.
Journal of molecular biology, 429(16), 2509-2527 (2017-07-04)
Arabinoxylans are constituents of the human diet. Although not utilizable by the human host, they can be fermented by colonic bacteria. The arabinoxylan backbone is decorated with arabinose side chains that may be substituted with ferulic acid, thus limiting depolymerization
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