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W222305

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Caroline C Gillis et al.
Cell host & microbe, 23(1), 54-64 (2017-12-26)
During Salmonella-induced gastroenteritis, mucosal inflammation creates a niche that favors the expansion of the pathogen population over the microbiota. Here, we show that Salmonella Typhimurium infection was accompanied by dysbiosis, decreased butyrate levels, and substantially elevated lactate levels in the...
Colin Charnock
Microorganisms, 9(1) (2021-01-07)
Plastic pollution has become one of the most critical environmental issues, as rapidly increasing production, compounded by persistence of plastic wastes in the environment, are outpacing efforts to keep ecosystems plastic-free. A switch to plastics more amenable to microbial attack...
Ahmed Aloulou et al.
Gastroenterology, 149(7), 1910-1919 (2015-09-04)
Pancreatic exocrine insufficiency (PEI) reduces pancreatic secretion of digestive enzymes, including lipases. Oral pancreatic enzyme replacement therapy (PERT) with pancreatin produces unsatisfactory results. The lipase 2 produced by the yeast Yarrowia lipolytica (YLLIP2; GenBank: AJ012632) might be used in PERT....
S P Câmara et al.
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 25(4), 303-317 (2019-01-17)
Autochthonous lactic acid bacteria may provide a means of promoting the quality and safety of traditional fermented food products, in particular, artisanal cheeses. Pico cheese is an artisanal, dairy specialty of the Azores in risk of disappearing. Efforts to maintain...
Markella Tzirita et al.
Journal of environmental sciences (China), 77, 174-188 (2018-12-24)
The presence of fats, oils and greases (FOGs) in wastewater can lead to many problems including blockages. Investigation of a bioaugmentation product, consisting of Bacillus spp., to degrade butter (1%, W/V) and olive oil (1%, V/V) was performed in aerobic...
G Jayanath et al.
International journal of biological macromolecules, 119, 393-401 (2018-06-17)
A novel esterase, designated as EstSP was identified by function based screening from a soil metagenomic fosmid library of solar saltern of Goa. EstSP gene of 1065 bp encoding a putative esterase of 354 amino acids showing 55% identity to esterase...
Philip C Christophersen et al.
The AAPS journal, 16(4), 875-883 (2014-05-31)
The effect of food intake on the release and degradation of peptide drugs from solid lipid particles is unknown and was therefore investigated in vitro using different fed state media in a lipolysis model. Desmopressin was used as a model...
Teresa Korniłłowicz-Kowalska et al.
International microbiology : the official journal of the Spanish Society for Microbiology, 23(2), 287-301 (2019-11-14)
The study characterizes the anamorphic Bjerkandera adusta strain CCBAS 930, including growth conditions, physiological properties, and enzymatic activities related to basic metabolism and specific properties coupled with the fungal secondary metabolism. It was established that the fungus grows in a...
José Luís Fachi et al.
Cell reports, 27(3), 750-761 (2019-04-18)
Antibiotic-induced dysbiosis is a key factor predisposing intestinal infection by Clostridium difficile. Here, we show that interventions that restore butyrate intestinal levels mitigate clinical and pathological features of C. difficile-induced colitis. Butyrate has no effect on C. difficile colonization or toxin production....
Renata de A B Assis et al.
Scientific reports, 7(1), 16133-16133 (2017-11-25)
The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect different hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on...
Mika Henrikki Sipponen et al.
Nature communications, 9(1), 2300-2300 (2018-06-14)
Dehydration reactions proceed readily in water-filled biological cells. Development of biocatalysts that mimic such compartmentalized reactions has been cumbersome due to the lack of low-cost nanomaterials and associated technologies. Here we show that cationic lignin nanospheres function as activating anchors...
Wu Liu et al.
Scientific reports, 7(1), 15711-15711 (2017-11-18)
As a kind of important biocatalysts, Pseudomonas lipases are commonly applied in various industrial fields. Pflip1, a new extracellular lipase gene from Pseudomonas. fluorescens Pf0-1, was first cloned and respectively expressed in Escherichia coli BL21(DE3) and Pichia pastoris KM71, the...
Fenghuan Zhao et al.
Journal of oleo science, 69(8), 893-905 (2020-08-08)
In this study, lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and organic groups modified SBA-15, and the enzymatic properties of the obtained immobilized TLL samples were investigated. 1) Activity of SBA-15-TLL at 2862.78 ± 293.24 U/g was...
Haobin Ye et al.
Cancer cell, 34(4), 659-673 (2018-10-03)
From an organismal perspective, cancer cell populations can be considered analogous to parasites that compete with the host for essential systemic resources such as glucose. Here, we employed leukemia models and human leukemia samples to document a form of adaptive...
Tao Zhang et al.
Microbial cell factories, 8, 67-67 (2009-12-24)
A metagenomic library was prepared using pCC2FOS vector containing about 3.0 Gbp of community DNA from the microbial assemblage of activated sludge. Screening of a part of the un-amplified library resulted in the finding of 1 unique lipolytic clone capable...
Éva Szentirmai et al.
Scientific reports, 9(1), 7035-7035 (2019-05-09)
Emerging evidence suggests that the intestinal microbiota is a source of sleep-promoting signals. Bacterial metabolites and components of the bacterial cell wall are likely to provide important links between the intestinal commensal flora and sleep-generating mechanisms in the brain. Butyrate...
Peter J Richards et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 92(2), 439-446 (2009-11-21)
Epidural adhesion formation is believed to be a central governing factor in the prevalence of pain after spinal surgery and is regarded as being the primary instigator of neural tethering, leading to complications during revision surgery. In this study, we...
Craig L Frank et al.
Molecules (Basel, Switzerland), 23(8) (2018-08-12)
Numerous free fatty acids (FFAs) are known to have potent antifungal effects. The mammalian epidermis contains both FFAs and multiple classes of fatty acid esters, including 1-monoacylglycerols and wax esters. We thus hypothesized that wax esters and 1-monoacylglycerols composed of...
Gytis Druteika et al.
World journal of microbiology & biotechnology, 36(3), 41-41 (2020-02-29)
This study presents a new microbial lipolytic enzyme GD-95RM designed via random mutagenesis using previously characterized GD-95 lipase as a template. The improvement in activity of GD-95 lipase was caused by E100K, F154V and V174I mutations. Compared with GD-95 lipase...
Maiara DA Costa Lima et al.
Journal of food protection, 81(11), 1800-1809 (2018-10-10)
This study evaluated the microbiological and physicochemical characteristics in different commercial brands of a Brazilian minimally ripened (coalho) cheese during 60 days of storage under refrigeration. Combinations of maximum and minimum values of water activity and pH determined in cheese...
Xihong Zhao et al.
Journal of the science of food and agriculture, 100(4), 1426-1435 (2019-11-12)
In this study, SBA-15 was functionalized by silane coupling reagents, then lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and the organically modified SBA-15 for diacylglycerol (DAG) production through glycerolysis. Diacylglycerol content of 54.77 ± 0.63%, and triacylglycerol (TAG) conversion...
Shahneela Mazhar et al.
Frontiers in microbiology, 11, 1533-1533 (2020-08-01)
Macrococcus caseolyticus subsp. caseolyticus is a Gram-positive, commensal organism documented to be present as a component of the secondary microflora in fermented foods such as Ragusano and Fontina cheeses and Cantonese sausage. In these products, the organism appears to play...
M H Sodergren et al.
Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society, 99(3), 153-161 (2010-11-04)
current studies suggest immunonutrition decreases the inflammatory process, infection rates and reduces length of hospital stay in surgical patients, however studies are often conducted on heterogeneous groups of patients with varying composition of the immuno-nutrition. We aim to investigate the...
Amal Salhi et al.
Biochimie, 169, 106-120 (2019-07-10)
Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they...
Lei Yuan et al.
Journal of Zhejiang University. Science. B, 19(8), 630-642 (2018-08-03)
The storage and transportation of raw milk at low temperatures promote the growth of psychrotrophic bacteria and the production of thermo-stable enzymes, which pose great threats to the quality and shelf-life of dairy products. Though many studies have been carried...
Fatma Rmili et al.
Biotechnology progress, 35(4), e2833-e2833 (2019-05-03)
A mesophilic bacterial culture, producing an extracellular alkaline lipase, was isolated from the gas-washing wastewaters generated from the Sfax phosphate plant of the Tunisian Chemical Group and identified as Staphylococcus capitis strain. The lipase, named S. capitis lipase (SCL), has...
François Guerineau et al.
Molecular biotechnology, 62(3), 168-176 (2020-01-02)
Arabidopsis hairy roots were used to produce human gastric lipase. When treated with 2,4-D, the hairy roots developed into thick organs that produced more protein than untreated roots. This was first assessed using green fluorescent protein-producing root lines from which...
Daniel Borda-Molina et al.
Antonie van Leeuwenhoek, 110(8), 1035-1051 (2017-05-20)
Microbial enrichments cultures are a useful strategy to speed up the search for enzymes that can be employed in industrial processes. Lipases have gained special attention because they show unique properties such as: broad substrate specificity, enantio- and regio-selectivity and...
Hitendra Kumar Patel et al.
Phytopathology, 107(6), 645-653 (2017-01-24)
Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae, an emerging pathogen of kiwifruit plants, has recently brought about major economic losses worldwide. Genetic studies on virulence functions of P. syringae pv. actinidiae have not yet been reported and there...
Sung Nam Kang et al.
International journal of pharmaceutics, 428(1-2), 76-81 (2012-03-13)
Tributyrin, a triglyceride analogue of butyrate, can act as a prodrug of an anticancer agent butyrate after being cleaved by intracellular enzymes. We recently demonstrated that the emulsion containing tributyrin as an inner oil phase possesses a potent anticancer activity....
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