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Timothy L Turner et al.
Applied microbiology and biotechnology, 99(19), 8023-8033 (2015-06-06)
Production of lactic acid from renewable sugars has received growing attention as lactic acid can be used for making renewable and bio-based plastics. However, most prior studies have focused on production of lactic acid from glucose despite that cellulosic hydrolysates
Na Wei et al.
ACS synthetic biology, 4(6), 707-713 (2015-01-15)
The inability of fermenting microorganisms to use mixed carbon components derived from lignocellulosic biomass is a major technical barrier that hinders the development of economically viable cellulosic biofuel production. In this study, we integrated the fermentation pathways of both hexose
F Monlau et al.
Bioresource technology, 187, 379-386 (2015-04-13)
The robust supramolecular structure of biomass often requires severe pretreatments conditions to produce soluble sugars. Nonetheless, these processes generate some inhibitory compounds (i.e. furans compounds and aliphatic acids) deriving mainly from sugars degradation. To avoid the inhibition of the biological
Lihui Liu et al.
Bioresource technology, 196, 662-667 (2015-08-28)
Autohydrolysis pretreatment with different severity factors was performed on poplar chips prior to chemi-thermomechanical pulping (CTMP) in order to investigate the change in pH value and its effect on the autohydrolysis pretreatment. The results showed that the dissolution amount of
Helena Osterholz et al.
Nature communications, 6, 7422-7422 (2015-06-19)
Dissolved organic matter (DOM) in the oceans constitutes a major carbon pool involved in global biogeochemical cycles. More than 96% of the marine DOM resists microbial degradation for thousands of years. The composition of this refractory DOM (RDOM) exhibits a
Stefan Zdraljevic et al.
Applied and environmental microbiology, 79(24), 7569-7582 (2013-09-17)
Organic acids derived from engineered microbes can replace fossil-derived chemicals in many applications. Fungal hosts are preferred for organic acid production because they tolerate lignocellulosic hydrolysates and low pH, allowing economic production and recovery of the free acid. However, cell
Mood Mohan et al.
Bioresource technology, 191, 244-252 (2015-05-23)
The aim of present study was to obtain total reducing sugars (TRS) from bamboo under subcritical water (SCW) treatment in a batch reactor at the temperature ranging from 170 °C to 220 °C and 40 min hydrolysis time. Experiments were
Guang Zeng et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(9), 6781-6788 (2015-04-04)
In the present study, we isolated and characterized a homogenous polysaccharide (GIAP1) from the alkaline extract of the roots of Glycyrrhiza inflata. The anti-tumor activity of GIAP1 toward human oral cancer SCC-25 cells and the underlying mechanisms were also examined
Jennifer M Hurley et al.
Cell systems, 7(6), 613-626 (2018-12-17)
Transcriptional and translational feedback loops in fungi and animals drive circadian rhythms in transcript levels that provide output from the clock, but post-transcriptional mechanisms also contribute. To determine the extent and underlying source of this regulation, we applied newly developed
Yu Gu et al.
Bioresource technology, 196, 116-122 (2015-08-02)
It has been proven that extrusion can change the structure of rice straw and increase biogas production, but the effect of a single pretreatment is limited. Ca(OH)2 pretreatment was used to enhance the enzyme hydrolysis and biogas production of extruded
Broder Rühmann et al.
Journal of chromatography. A, 1350, 44-50 (2014-05-28)
A fast carbohydrate screening platform processible in 96-well format is described. The method is suitable for the determination of various carbohydrates out of complex mixtures as obtained by acidic hydrolysis of carbohydrates polymers. The chromatographic conditions for an efficient separation
Alessandra Procentese et al.
Bioresource technology, 192, 31-36 (2015-05-26)
Ionic liquid (ILs) pretreatment of lignocellulosic biomass has attracted broad scientific interest, despite high costs, possible toxicity and energy intensive recycling. An alternative group of ionic solvents with similar physicochemical properties are deep eutectic solvents (DESs). Corncob residues were pretreated
Kazuhiko Kurosawa et al.
Bioresource technology, 193, 206-212 (2015-07-05)
Extracting lipids from oleaginous microbial cells in a cost effective and environmentally compatible manner remains a critical challenge in developing manufacturing paradigms for advanced liquid biofuels. In this study, a new approach using microbial growth inhibitors from lignocellulose-derived feedstocks was
Xin-Jun Yu et al.
Applied biochemistry and biotechnology, 177(6), 1229-1240 (2015-08-25)
High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in
Zhen-Bin Wang et al.
Carbohydrate polymers, 109, 49-55 (2014-05-13)
Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively. PL-N mainly comprised xylose and arabinose with a high molecular weight
Guillaume Larivière-Gauthier et al.
Journal of food protection, 77(12), 2121-2128 (2014-12-05)
Following the 2008 Canadian listeriosis outbreak associated with ready-to-eat (RTE) meat products, regulations on the presence of Listeria monocytogenes in RTE food production facilities were modified by Health Canada, confirming the need to control this pathogen, not only in the
James D Oliver et al.
Biotechnology and applied biochemistry, 62(3), 329-342 (2014-07-22)
Free-solution capillary electrophoresis (CE), or capillary zone electrophoresis, with direct UV detection was used for the first time for the determination of mono- and disaccharides, sugar alcohols, and ethanol in fermentation broths. Sample preparation proved to be minimal: no derivatization
Jun Zhou et al.
Journal of ethnopharmacology, 164, 229-238 (2015-02-24)
Rehmannia glutinosa (Gaertn.) DC. (RG) has been widely used as traditional Chinese herbal medicine for treatment of diabetes and its complications. The polysaccharide fraction of RG has been proposed to possess hypoglycemic effect by intraperitoneal administration, however, the mechanisms responsible
Fuqing Xu et al.
Bioresource technology, 200, 753-760 (2015-11-18)
To select a proper inoculum for the solid state anaerobic digestion (SS-AD) of yard trimmings, digestate from solid anaerobic digesters and dewatered effluent from liquid anaerobic digesters were compared at substrate-to-inoculum (S/I) ratios from 0.2 to 2 (dry basis), and
Hyun Yong Shin et al.
Biotechnology for biofuels, 8, 176-176 (2015-11-05)
The yeast Saccharomyces cerevisiae is unable to ferment pentose sugars like d-xylose. Through the introduction of the respective metabolic pathway, S. cerevisiae is able to ferment xylose but first utilizes d-glucose before the d-xylose can be transported and metabolized. Low
Shuangquan Yao et al.
Bioresource technology, 185, 21-27 (2015-03-10)
To reduce the degradation of cellulose and obtain high molecular weight of hemicellulose from the extracts, pH pre-corrected hot water pretreatment was developed by employing sodium hydroxide (3.9mol/L). The response surface model was established to optimize the extraction process. The
Chao Wu et al.
Plant physiology, 167(2), 586-599 (2014-12-17)
Integrated and genome-based flux balance analysis, metabolomics, and (13)C-label profiling of phototrophic and heterotrophic metabolism in Chlorella protothecoides, an oleaginous green alga for biofuel. The green alga Chlorella protothecoides, capable of autotrophic and heterotrophic growth with rapid lipid synthesis, is
Kyle S Landry et al.
Food microbiology, 51, 10-17 (2015-07-19)
The incidence of foodborne illness associated with the consumption of fresh produce has continued to increase over the past decade. Sprouts, such as mung bean, alfalfa, radish, and broccoli, are minimally processed and have been sources for foodborne illness. Currently
I S M Rafiqul et al.
Applied biochemistry and biotechnology, 176(4), 1071-1083 (2015-04-24)
Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from
Xiaobei Li et al.
Food chemistry, 166, 358-364 (2014-07-24)
The aim of this research was to study effects of drying methods on the tasty compounds of Pleurotus eryngii, a common commercial edible fungus. In order to maximally maintain the taste of P. eryngii, several different drying methods, including hot
Ava A Greenwood et al.
Biotechnology for biofuels, 8, 26-26 (2015-05-13)
Acid hydrolysis is a popular pretreatment for removing hemicellulose from lignocelluloses in order to produce a digestible substrate for enzymatic saccharification. In this work, a novel model for the dilute acid hydrolysis of hemicellulose within sugarcane bagasse is presented and
Yanmei Zou et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5179-5186 (2015-02-06)
In the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) from Andrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells
Rongjun He et al.
International journal of biological macromolecules, 78, 56-61 (2015-04-04)
Three polysaccharides (SAP30, SAP60 and SAP80) were separated from the body of Edwardsia sipunculoides by tissue homogenate and papain hydrolysis. Total soluble sugar contents, monosaccharide compositions, antioxidant and antitumor activities in vitro of those polysaccharides were investigated, respectively. Results showed
Belisa B de Sales et al.
Biotechnology letters, 37(10), 1973-1982 (2015-06-20)
Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae. An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ)
Stephen R Hughes et al.
Journal of laboratory automation, 20(6), 621-635 (2015-02-28)
A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed and transformed into Saccharomyces cerevisiae to demonstrate feasibility as a stable protein expression system in
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