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  • Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis.

Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis.

The New phytologist (2018-02-21)
Jacob Krüger Jensen, Marta Busse-Wicher, Christian Peter Poulsen, Jonatan Ulrik Fangel, Peter James Smith, Jeong-Yeh Yang, Maria-Jesus Peña, Malene Hessellund Dinesen, Helle Juel Martens, Michael Melkonian, Gane Ka-Shu Wong, Kelley W Moremen, Curtis Gene Wilkerson, Henrik Vibe Scheller, Paul Dupree, Peter Ulvskov, Breeanna Rae Urbanowicz, Jesper Harholt
ABSTRACT

Insights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand the evolving structure-function relationships of the plant cell wall, it is imperative to trace the origin of its different components. The present study is focused on plant 1,4-β-xylan, tracing its evolutionary origin by genome and transcriptome mining followed by phylogenetic analysis, utilizing a large selection of plants and algae. It substantiates the findings by heterologous expression and biochemical characterization of a charophyte alga xylan synthase. Of the 12 known gene classes involved in 1,4-β-xylan formation, XYS1/IRX10 in plants, IRX7, IRX8, IRX9, IRX14 and GUX occurred for the first time in charophyte algae. An XYS1/IRX10 ortholog from Klebsormidium flaccidum, designated K. flaccidumXYLAN SYNTHASE-1 (KfXYS1), possesses 1,4-β-xylan synthase activity, and 1,4-β-xylan occurs in the K. flaccidum cell wall. These data suggest that plant 1,4-β-xylan originated in charophytes and shed light on the origin of one of the key cell wall innovations to occur in charophyte algae, facilitating terrestrialization and emergence of polysaccharide-based plant cell walls.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Xylobiose, ≥90% (HPLC)