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Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System.

Materials (Basel, Switzerland) (2018-09-16)
Jumana Alhamdi, Emily Jacobs, Gloria Gronowicz, Nadia Benkirane-Jessel, Marja Hurley, Liisa Kuhn
ABSTRACT

Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) and the bone-like apatite barrier layer that make up the delivery system, were varied. The effects of the structural variations of the coating on the kinetics of cell access to a cytotoxic factor delivered by the layered structure were evaluated. FGF-2 was adsorbed into the outer PEM, and cytotoxic antimycin-A (AntiA) was adsorbed to the substrate below the barrier layer to detect the timing of the cell access. While MC3T3-E1 osteoprogenitor cells accessed AntiA after three days, the RAW 264.7 macrophage access occurred within 4 h, unless the PEM layer was removed, in which case the results were reversed. Pits were created in the coating by the RAW 264.7 macrophages and initiated delivery, while the osteoprogenitor cell access to drugs occurred through a solution-mediated coating dissolution, at junctions between the islands of crystals. Macrophage-mediated degradation is therefore a mechanism that controls drug release from coatings containing bioinspired apatite.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly-L-lysine hydrobromide, mol wt 30,000-70,000
Sigma-Aldrich
Poly-L-glutamic acid sodium salt, mol wt 15,000-50,000