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An interspecies barrier to tetraploid complementation and chimera formation.

Scientific reports (2018-10-18)
Tomoyuki Yamaguchi, Hideyuki Sato, Toshihiro Kobayashi, Megumi Kato-Itoh, Teppei Goto, Hiromasa Hara, Naoaki Mizuno, Ayaka Yanagida, Ayumi Umino, Sanae Hamanaka, Fabian Suchy, Hideki Masaki, Yasunori Ota, Masumi Hirabayashi, Hiromitsu Nakauchi
ABSTRACT

To study development of the conceptus in xenogeneic environments, we assessed interspecies chimera formation as well as tetraploid complementation between mouse and rat. Overall contribution of donor PSC-derived cells was lower in interspecies chimeras than in intraspecies chimeras, and high donor chimerism was associated with anomalies or embryonic death. Organ to organ variation in donor chimerism was greater in interspecies chimeras than in intraspecies chimeras, suggesting species-specific affinity differences among interacting molecules necessary for organogenesis. In interspecies tetraploid complementation, embryo development was near normal until the stage of placental formation, after which no embryos survived.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Sigma-Aldrich
D-Mannitol, ≥98%
Sigma-Aldrich
Poly(vinyl alcohol), 87-90% hydrolyzed, average mol wt 30,000-70,000