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The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development.

Cell (2018-10-16)
Andrew T N Tebbenkamp, Luis Varela, Jinmyung Choi, Miguel I Paredes, Alice M Giani, Jae Eun Song, Matija Sestan-Pesa, Daniel Franjic, André M M Sousa, Zhong-Wu Liu, Mingfeng Li, Candace Bichsel, Marco Koch, Klara Szigeti-Buck, Fuchen Liu, Zhuo Li, Yuka I Kawasawa, Constantinos D Paspalas, Yann S Mineur, Paolo Prontera, Giuseppe Merla, Marina R Picciotto, Amy F T Arnsten, Tamas L Horvath, Nenad Sestan

Despite the known causality of copy-number variations (CNVs) to human neurodevelopmental disorders, the mechanisms behind each gene's contribution to the constellation of neural phenotypes remain elusive. Here, we investigated the 7q11.23 CNV, whose hemideletion causes Williams syndrome (WS), and uncovered that mitochondrial dysfunction participates in WS pathogenesis. Dysfunction is facilitated in part by the 7q11.23 protein DNAJC30, which interacts with mitochondrial ATP-synthase machinery. Removal of Dnajc30 in mice resulted in hypofunctional mitochondria, diminished morphological features of neocortical pyramidal neurons, and altered behaviors reminiscent of WS. The mitochondrial features are consistent with our observations of decreased integrity of oxidative phosphorylation supercomplexes and ATP-synthase dimers in WS. Thus, we identify DNAJC30 as an auxiliary component of ATP-synthase machinery and reveal mitochondrial maladies as underlying certain defects in brain development and function associated with WS.

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