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A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells.

Science (New York, N.Y.) (1994-08-05)
J Han, J D Lee, L Bibbs, R J Ulevitch
ABSTRACT

Mammalian cells respond to endotoxic lipopolysaccharide (LPS) by activation of protein kinase cascades that lead to new gene expression. A protein kinase, p38, that was tyrosine phosphorylated in response to LPS, was cloned. The p38 enzyme and the product of the Saccharomyces cerevisiae HOG1 gene, which are both members of the mitogen-activated protein (MAP) kinase family, have sequences at and adjacent to critical phosphorylation sites that distinguish these proteins from most other MAP kinase family members. Both HOG1 and p38 are tyrosine phosphorylated after extracellular changes in osmolarity. These findings link a signaling pathway in mammalian cells with a pathway in yeast that is responsive to physiological stress.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse, ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-phospho-ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
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Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal, clone ERK-NP2, purified from hybridoma cell culture
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Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal, clone ERK-PY193, purified from hybridoma cell culture
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Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit, whole antiserum
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PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb, liquid, Calbiochem®
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Anti-p38 MAP Kinase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
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Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse, clone 1B3B9, purified immunoglobulin, buffered aqueous solution
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Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
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p38α, active, GST tagged human, PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody, Mouse monoclonal, clone MAPK-YT, purified from hybridoma cell culture