• Home
  • Search Results
  • Dopamine-Evoked Synaptic Regulation in the Nucleus Accumbens Requires Astrocyte Activity.

Dopamine-Evoked Synaptic Regulation in the Nucleus Accumbens Requires Astrocyte Activity.

Neuron (2020-01-20)
Michelle Corkrum, Ana Covelo, Justin Lines, Luigi Bellocchio, Marc Pisansky, Kelvin Loke, Ruth Quintana, Patrick E Rothwell, Rafael Lujan, Giovanni Marsicano, Eduardo D Martin, Mark J Thomas, Paulo Kofuji, Alfonso Araque

Dopamine is involved in physiological processes like learning and memory, motor control and reward, and pathological conditions such as Parkinson's disease and addiction. In contrast to the extensive studies on neurons, astrocyte involvement in dopaminergic signaling remains largely unknown. Using transgenic mice, optogenetics, and pharmacogenetics, we studied the role of astrocytes on the dopaminergic system. We show that in freely behaving mice, astrocytes in the nucleus accumbens (NAc), a key reward center in the brain, respond with Ca2+ elevations to synaptically released dopamine, a phenomenon enhanced by amphetamine. In brain slices, synaptically released dopamine increases astrocyte Ca2+, stimulates ATP/adenosine release, and depresses excitatory synaptic transmission through activation of presynaptic A1 receptors. Amphetamine depresses neurotransmission through stimulation of astrocytes and the consequent A1 receptor activation. Furthermore, astrocytes modulate the acute behavioral psychomotor effects of amphetamine. Therefore, astrocytes mediate the dopamine- and amphetamine-induced synaptic regulation, revealing a novel cellular pathway in the brain reward system.

Product Number
Product Description

AM251, >98% (HPLC), solid
Anti-NeuN Antibody, clone A60, clone A60, Chemicon®, from mouse
Anti-NG2 Antibody, clone 132.38, clone 132.38, Upstate®, from mouse
Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
6-Methyl-2-(phenylethynyl)pyridine hydrochloride, ≥98% (HPLC)
Picrotoxin, powder
LY-367385 hydrochloride, ≥98% (HPLC)