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Changes in the Content of Phenolic Compounds and Biological Activity in Traditional Mexican Herbal Infusions with Different Drying Methods.

Molecules (Basel, Switzerland) (2020-04-05)
Sandra N Jimenez-Garcia, Moisés A Vazquez-Cruz, Xóchitl S Ramirez-Gomez, Vicente Beltran-Campos, Luis M Contreras-Medina, Juan F Garcia-Trejo, Ana A Feregrino-Pérez
ABSTRACT

Mexican spices are used in the supplementation of the human diet and as medicinal herbs for the particularly high amounts of compounds capable of deactivating free radicals. In addition, these spices can have beneficial effects on chronic, no-transmissible diseases such as type II diabetes and hypertension arterial. The objective of this study is to determine the content of phenolic compounds on the antioxidant activity and inhibitory enzymes of α-amylase, α-glucosidase and angiotensin-converting enzyme in melissa, peppermint, thyme and mint, which are subjected to microwave drying, conventional and freeze-drying to be used as alternative treatments. Spices were evaluated to determine total phenols, flavonoids, tannins, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), (2,2'-azino-bis- (3-ethyl benzothiazolin-6-ammonium sulphonate) (ABTS) and Ferric Reducing/Antioxidant Power (FRAP), enzymatic activity. The investigation showed that conventional drying caused a decrease in antioxidant properties and inhibitory activity, in some species, while remained preserved in microwave drying and freeze-drying. The activity of polyphenol oxides and peroxidase decreases with high temperatures and these increase with the use of cold temperatures. This study aims to determine the extent of optimal drying required to preserve phenolic compounds, and the positive effect on antioxidant activity and enzymatic activity in in vitro models, which will produce benefits for the infusion processing industry and the pharmaceutical industry.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-Glucosidase from Bacillus stearothermophilus, lyophilized powder, ≥50 units/mg protein
Sigma-Aldrich
N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly
Sigma-Aldrich
Anti-α-Amylase antibody produced in rabbit, fractionated antiserum, lyophilized powder
BRAND® 96-well microplate, U-bottom, round bottom, non-sterile