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Potential use of two aryl sulfotransferase cell-surface display systems to detoxify the endocrine disruptor bisphenol A.

Biochemical and biophysical research communications (2020-06-10)
Pakjira Nanudorn, Sirinthra Thiengmag, Wirongrong Whangsuk, Skorn Mongkolsuk, Suvit Loprasert
ABSTRACT

Bisphenol A (BPA) is one of the most common toxic endocrine disruptors in the environment. A fast, efficient and environmental-friendly method for BPA detoxification is urgently needed. In this study, we show that the enzymatic transformation of BPA into a non-estrogenic BPA sulfate can be performed by the aryl sulfotransferase (ASTB) from Desulfitobacterium hafniense. We developed and compared two Escherichia coli ASTB cell-surface displaying systems using the outer membrane porin F (OprF) and the lipoprotein outer membrane A (Lpp-OmpA) as carriers. The surface localization of both fusion proteins was confirmed by Western blot and flow cytometry analysis as well as the enzymatic activity assay of the outer membrane fractions. Unfortunately, Lpp-OmpA-ASTB cells had an adverse effect on cell growth. In contrast, the OprF-ASTB cell biocatalyst was stable, expressing 70% of enzyme activity for 7 days. It also efficiently sulfated 90% of 5 mM BPA (1 mg/mL) in wastewater within 6 h.

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BRAND® 96-well microplate, U-bottom, round bottom, non-sterile