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Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model.

Antimicrobial agents and chemotherapy (2009-06-24)
Kenji Kurokawa, Hiroshi Hamamoto, Miki Matsuo, Satoshi Nishida, Noriko Yamane, Bok Luel Lee, Kazuhisa Murakami, Hideki Maki, Kazuhisa Sekimizu
ABSTRACT

The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of D-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.

MATERIALS
Product Number
Brand
Product Description

Supelco
Norfloxacin, analytical standard, ≥98% (TLC)
Sigma-Aldrich
D-Cycloserine
Sigma-Aldrich
D-Cycloserine, synthetic
Supelco
Norfloxacin, VETRANAL®, analytical standard
Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)